STUDY ON GROWTH AND ADENOSINE NUCLEOTIDES OF MARINE AND TERESTRIAL PSYCHROTROPHIC BACTERIA

海洋和陆地耐冷细菌的生长和腺苷核苷酸的研究

• 批准号: 07806028
• 负责人: MORII Hideaki
• 金额: $ 1.34万
• 依托单位: NAGASAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07806028/
• 关键词: PSYCHROTROPHIC BACTERIA; GROWTH; ADENOSUNE NUCLEOTIDES

The mechanism that organisms can live at low temperature is not clear. Especially, the study for nucleic acid precursors has not been almost performed until this time because the method for the speedy and accurate analysis has not been perfected. The methods for determination of adenylate concentrations in bacterial culture were examined and perfected. Scecondly, marine and terrestrial psychrotrophic bacteria were isolated from cold environments, and growth and change with growth time in adenylate concentrations were studied especially for psychrotrophic Pseudomonas spp. In the results, the psychrotrophic Pseudomonas spp.were divided into two groups on the basis of growth pattern and optimum growth temperature (Group I and II strains having optimum growth temperature below and above 25ﾟC). The two groups were different from effects of magnesium and glucose on growth, production of acid from glucose, utilization of pyruvate etc.as sole carbon source. The level of adenosine nucleotides i … More n Group I was generally highest in AMP,moderate in ADP,and lowest in ATP during growth, regardless of the growth temperatures. Almost all adenosine nucleotides in Group II were occupied by AMP through the entire growth period, independently of the growth temperatures. The adenylate energy charge (ATP+1/2ADP/ATP+ADP+AMP) during the growth phase was extremely low value (approach to the zero) in Group II.In Group I grown at the temperatures of an optimum temperature or below, adenylate concentrations in the culture increased with elongation of incubation time. In Group II,AMP concentration rose quickly, reached a maximum and then fell abruptly to the base during the logarithmic and stationary phases for growth. The similar results were also recognized in different inorganic and organic media, oxygen tentions, and pHs. These results suggest that Groups I and II are different evolutional groups, and that the growth at low temperature in psychrotrophic bacteria is connected with adenylate metabolisms. Less

生物体在低温下生存的机制尚不清楚。特别是，由于快速、准确的分析方法尚未完善，对核酸前体的研究直到此时才基本进行。检验并完善了细菌培养物中腺苷酸浓度的测定方法。其次，从寒冷环境中分离出海洋和陆地耐冷细菌，并研究了耐冷假单胞菌的生长和腺苷酸浓度随生长时间的变化。结果，耐冷假单胞菌根据生长方式和最适生长温度分为两组（I组和II组菌株的最适生长温度低于和高于25℃）。镁和葡萄糖对生长、葡萄糖产酸、丙酮酸作为唯一碳源的利用等方面的影响两组不同。无论生长温度如何，在生长过程中，第一组的腺苷核苷酸水平通常在 AMP 中最高，在 ADP 中中等，在 ATP 中最低。在整个生长期间，第二组中的几乎所有腺苷核苷酸都被 AMP 占据，与生长温度无关。 II组在生长阶段的腺苷酸能量电荷(ATP+1/2ADP/ATP+ADP+AMP)极低(接近于零)。在最适温度或以下的温度下生长的I组中，培养物中的腺苷酸浓度随着培养时间的延长而增加。在第二组中，AMP浓度迅速上升，达到最大值，然后在生长的对数期和稳定期突然降至最低点。在不同的无机和有机介质、氧张力和 pH 值中也得到了类似的结果。这些结果表明，I类和II类是不同的进化类群，耐冷细菌的低温生长与腺苷酸代谢有关。较少的