Studies on the phenotypic expression of embryonic cells from teleost

本研究的目的是建立比目鱼胚胎细胞的培养程序，以揭示其在培养条件下分化过程中的调控系统。作为第一步，研制了用于细胞培养的时间圈视频记录系统。在此基础上，本研究以牙鲆胚胎为主要材料进行了细胞培养，同时还成功地利用了团头鱼和石斑鱼的胚胎进行细胞培养。本研究建立的胚胎细胞培养方法简单有效。胚性细胞培养的最佳培养基为L-15添加FBS，但该培养基不适合用于分析细胞分化的控制因素。因此，在不久的将来，需要发展无血清培养的方法，从莫拉拉胚胎分化为色素细胞、神经细胞、上皮细胞和成纤维细胞。尤其是神经胚后胚胎，分化细胞变异增多，细胞黏附明显。这些簇由神经细胞组成网络。牙槽鱼体内发育过程中，色素细胞在这种细胞团中先后分化，并在植入后出现两种不同的形态。

The objectives of this study is to establish the procedure of embryo cell from flatfish to reveal the control system in their differentiation process under culture conditions. The recording system with time laps video for cell culture was developed as the first step. Then, the embryos of Japanese flounder Paralichthys olivaceus were mainly used for this study, but those from red sea bream Pagrus major and black sea bream Acanthopagrus schlegeli were also succeeded in cell culture. The procedure of cell culture from developing embryos which was developed in this study was extremely easy and effective. The best medium for embryo cell culture was L-15 supplemented FBS.But this medium is not suitable to analyze the control factors of cell differentiation. Therefor, it is needed to develop the procedures using the medium without serum in near future.From molura embryos, pigmet cells, nerve cells, epithelial cells and fibroblastic cells differentiated. Especially, embryos after neurula stage, variation of differentiated cells increased, and remarkable cell adhesions occurred. These clusters were networked by nerve cells. Pigment cells differentiated successively in such cell cluster with two different appearance in timing after plating corresponding in vivo development in Japanese flounder.