Studies on the phenotypic expression of embryonic cells from teleost

硬骨鱼胚胎细胞表型表达的研究

• 批准号: 07806026
• 负责人: SEIKAI Tadahisa
• 金额: $ 1.41万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07806026/
• 关键词: Embryonic cells; Cell culture; Phenotypic expression; Japanese flounder; Pigment cell; 胚細胞; 分化; 生物時計; 海産魚; 発生段階; L-15培地; ビタミンA; 海産魚類; 細胞分化

The objectives of this study is to establish the procedure of embryo cell from flatfish to reveal the control system in their differentiation process under culture conditions. The recording system with time laps video for cell culture was developed as the first step. Then, the embryos of Japanese flounder Paralichthys olivaceus were mainly used for this study, but those from red sea bream Pagrus major and black sea bream Acanthopagrus schlegeli were also succeeded in cell culture. The procedure of cell culture from developing embryos which was developed in this study was extremely easy and effective. The best medium for embryo cell culture was L-15 supplemented FBS.But this medium is not suitable to analyze the control factors of cell differentiation. Therefor, it is needed to develop the procedures using the medium without serum in near future.From molura embryos, pigmet cells, nerve cells, epithelial cells and fibroblastic cells differentiated. Especially, embryos after neurula stage, variation of differentiated cells increased, and remarkable cell adhesions occurred. These clusters were networked by nerve cells. Pigment cells differentiated successively in such cell cluster with two different appearance in timing after plating corresponding in vivo development in Japanese flounder.

本研究的目的是建立比目鱼胚胎细胞的培养过程，揭示在培养条件下比目鱼胚胎细胞分化过程中的控制系统。第一步是开发细胞培养的时间圈视频记录系统。然后，以日本牙鲆（parichthys olivaceus）胚胎为主要材料，对红鲷（Pagrus major）和黑鲷（Acanthopagrus schlegeli）胚胎也进行了细胞培养。本研究所建立的胚胎细胞培养方法简单有效。培养胚胎细胞的最佳培养基为L-15 + FBS。但这种培养基不适合分析细胞分化的控制因素。因此，在不久的将来，需要开发无血清培养基的方法。色素细胞、神经细胞、上皮细胞和成纤维细胞从痣胚分化而来。特别是神经期后的胚胎，分化细胞变异增加，出现了明显的细胞粘连。这些簇是由神经细胞组成的。在牙鲆的体内发育过程中，色素细胞在细胞群中先后分化，形成两种不同时间形态的细胞群。