Endothelial Cell-Pericyte Interdigitation is Mediator of Angiogenesis?
内皮细胞-周细胞交叉是血管生成的介质?
基本信息
- 批准号:07806042
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Under in vitro conditions pericytes have been shown to inhibit the proliferation of cultured endothelial cells. This inhibitory effect of pericytes appeared to be mediated by a activated form of TGFbeta depended on the formation of cell-cell contacts between endothelial cells and pericytes, and is mediated by uPA-plasmin activity. However, the endothelial cell and pericyte contacts during coculture have been detected only light microscopy, and the detailed structures of these contact has been unresolved. Meantime, in vivo angiogenesis, immature capillaries possess many endothelial cell and pericyte contacts, such as endothelial cell-pericyte interdigitations (EPI). I have demonstrated that the number of EPI increases with ongoing maturation of newly formed capillaries, and suggested pericytes do indeed to inhibit endothelial cells proliferation in a manner requiring the physical proximity effort by the EPI.The present study revealed in angiogenic sites that uPA expression was preferentially in EPI and TGFbeta was present throughout the stroma including EPI.These findings suggest a functional interplay between uPA expression and endothelial cells maturation, and support the idea that endothelial cells proliferating inhibitor function by TGFbeta activated by uPA during angiogenesis to mediate at EPI.
在体外条件下,周细胞已显示出抑制培养的内皮细胞的增殖。周细胞的这种抑制作用似乎是由活化形式的TGF β介导的,其依赖于内皮细胞和周细胞之间细胞-细胞接触的形成,并且是由uPA-纤溶酶活性介导的。然而,在共培养过程中,内皮细胞和周细胞的接触仅在光学显微镜下检测到,这些接触的详细结构尚未得到解决。同时,在体内血管生成过程中,未成熟的毛细血管存在着内皮细胞与周细胞的相互接触,如内皮细胞与周细胞的相互连接(EPI)。我已经证明EPI的数量随着新形成的毛细血管的不断成熟而增加,本研究显示,在血管生成部位,uPA表达优先在EPI中,TGF β存在于包括EPI在内的整个基质中,这些发现表明uPA表达与内皮细胞增殖之间存在功能性相互作用。支持内皮细胞增殖抑制因子在血管生成过程中通过uPA激活TGF β介导EPI发挥作用的观点。
项目成果
期刊论文数量(21)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Wakui,S.et al.: "Epithelial glycoconjugates of canine efferent ductules" Journal of Anatomy. 188. 541-546 (1996)
Wakui,S.et al.:“犬传出导管的上皮糖缀合物”解剖学杂志。
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Wakui,S.: "Immunocytochemical localization of epidermal growth factor receptor in the rhesus monkey prostate." Anatomia Histologia Embryologia. 25. 109-111 (1996)
Wakui,S.:“恒河猴前列腺中表皮生长因子受体的免疫细胞化学定位。”
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Wakui, S.: "Lectin histochemical evaluation of glycoconjugates in canine efferent ductules" : Journal of Anatomy. (in press). (1996)
Wakui,S.:“犬传出导管中糖缀合物的凝集素组织化学评估”:解剖学杂志。
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- 影响因子:0
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Wakui, S.Furusato, M.Yokoo, K.Ushigome, S.: "Testicular efferent ductule cyst of a dog." Veterinary Pathology. (in press).
Wakui,S.Furusato,M.Yokoo,K.Ushigome,S.:“狗的睾丸传出小管囊肿。”
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Wakui, S.Furusato, M.Sasaki, S.Ohshige, H.Ushigome, S.: (Editors : Tsuchiya M., Asano M., Kamiya A) : Microcirculation Annual '1995. Nihon Igakukan, (1995)
Wakui,S.Furusato,M.Sasaki,S.Ohshige,H.Ushigome,S.:(编辑:Tsuchiya M.,Asano M.,Kamiya A):微循环年鉴1995。
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WAKUI Shin其他文献
WAKUI Shin的其他文献
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{{ truncateString('WAKUI Shin', 18)}}的其他基金
Three-dimensional ultrastructural study of Endothelial cells and pericytes interdigitation in angiogenesis
血管生成中内皮细胞和周细胞交错的三维超微结构研究
- 批准号:
21580371 - 财政年份:2009
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Role of endothelial cells and pericyte interdigitation in angiogenesis
内皮细胞和周细胞交错在血管生成中的作用
- 批准号:
15580267 - 财政年份:2003
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Endothelial Cell - Pericyte Interdigitation in Angiogenesis
血管生成中的内皮细胞-周细胞交叉
- 批准号:
12660278 - 财政年份:2000
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Endothelial Cell-Pericyte Interdigitation in Angiogenesis
血管生成中的内皮细胞-周细胞交叉
- 批准号:
09660332 - 财政年份:1997
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)