Endothelial Cell - Pericyte Interdigitation in Angiogenesis

血管生成中的内皮细胞-周细胞交叉

• 批准号: 12660278
• 负责人: WAKUI Shin
• 金额: $ 2.56万
• 依托单位: Azabu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660278/
• 关键词: Angiogenesis; Endothelial cell pericyte interdigitation; EPI; 血管新生; マイクロアレイ法

Vascular endothelial growth factor (VEGF) is a dimeric glycoprotein that specifically increases vascular permeability and stimulates angiogenesis. Recently we demonstrated a high level of VEGF and VEGF mRNA expression in N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN)-induced rat bladder carcinomas with abundant fenestrated capillaries. We speculated that the expression of VEGF might be involved in the induction or maintenance of capillary fenestration, and examined ultrastructurally the morphological alteration of blood capillaries in BBN-induced rat bladder carcinoma following the administration of neutralizing antibody against VEGF. Five minutes after administration, the fenestrated endothelial cell ratio and the number of fenestrated decreased significantly, and continued to decrease with time reaching a minimum at 10 minutes after administration. The ratio thereafter gradually increased and at 30 minutes returned to its control condition. These results showed the specific inhibition of VEGF bioactivity potently induced to close the capillary fenestrations. We can suggest that VEGF synthesized by BBN-induced rat bladder carcinoma cells specifically induces and maintains the tumor capillary fenestrations.

血管内皮生长因子（VEGF）是一种二聚体糖蛋白，特异性增加血管通透性并刺激血管生成。最近，我们证明了高水平的血管内皮生长因子和血管内皮生长因子mRNA表达的N-丁基-N-（4-羟丁基）亚硝胺（BBN）诱导的大鼠膀胱癌与丰富的开窗毛细血管。我们推测VEGF的表达可能参与了毛细血管窗的诱导或维持，并在超微结构上观察了BBN诱导的大鼠膀胱癌中给予VEGF中和抗体后毛细血管的形态学变化。给药后5 min，有孔内皮细胞比率和有孔内皮细胞数均显著下降，并随时间持续下降，给药后10 min达到最低值。此后，该比率逐渐增加，并在30分钟时恢复到其对照条件。这些结果表明，VEGF生物活性的特异性抑制有效地诱导关闭毛细血管窗。我们可以认为，由BBN诱导的大鼠膀胱癌细胞合成的VEGF特异性地诱导和维持肿瘤毛细血管窗。

项目成果

Inoue,K., Shirota,K., Kami-ie,J., Ohtake,S., Wakui,S., Machida,S.: "Atypical membranoproliferative glomerulonephritis in a cat"Veterinary Pathology. 38. 468-470 (2001)

Inoue,K.、Shirota,K.、Kami-ie,J.、Ohtake,S.、Wakui,S.、Machida,S.：“猫的非典型膜增生性肾小球肾炎”兽医病理学。

武藤朋子 他: "癌押制遺伝子P53"日本獣医がん研究会誌. 3. 6-10 (2001)

Tomoko Muto 等：“癌症抑制基因 P53”日本兽医癌症研究会杂志 3. 6-10 (2001)。

Muto,T., Takahashi,H., Hano,H., Wakui,S., Furusato,M.: "Estrous cyclicity and ovarian follicles in female rats after prenatal exposure to 3, 3', 4, 4', 5-pentachlorobiphenyl"Toxicology Letters. (in press). (2003)

Muto,T.、Takahashi,H.、Hano,H.、Wakui,S.、Furusato,M.：“产前暴露于 3、3、4、4、5- 后雌性大鼠的发情周期和卵泡

Muto, T. et al.: "In-utero and lactational exposure of 3,3',4,4',5-pentachlorobiphenyl modulate dimethlbenz[a]anthracene-induced rat mammary carcinogenesis"Journal of Toxicologic Pathology. 14. 213-224 (2001)

Muto, T. 等人：“子宫内和哺乳期暴露 3,3,4,4,5-五氯联苯调节二甲基苯并[a]蒽诱导的大鼠乳腺癌发生”毒理学病理学杂志。

古里征国 他: "Nuclear body"Mebio. 17. 125-127 (2000)

Seikuni Furusato 等：“核体”Mebio 17. 125-127 (2000)。