Three-dimensional ultrastructural study of Endothelial cells and pericytes interdigitation in angiogenesis

血管生成中内皮细胞和周细胞交错的三维超微结构研究

• 批准号: 21580371
• 负责人: WAKUI Shin
• 金额: $ 3.24万
• 依托单位: Azabu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580371/
• 关键词: 病理; 血管新生制御; 血管新生; 血管内皮細胞; 血管周細胞; 細胞質突起相互陥入; 周細胞; 相互陥入部; 三次元電顕免疫学

Endothelial cells and pericytes play critical role in angiogenesis by the angiopoietin system. Endothelial cell and pericyte interdigitations (EPI), consisting of cytoplasmic projections of pericytes and corresponding endothelial indentations, are frequently present at angiogenic sites. Rats subcutaneous implantation of a thermoreversible gelation polymer discs were inqubated in vitro 24-72hr. The capillary density increased to a peak on 48hr. A number of EPI were observed to a peak on 72hr. Serial sectioning electron microscopy immunohistochemical analysis revealed that Angiopoietin-1 localized at Pericytes and EPI. Quantitative RT-PCR analysis revealed that the level of Angiopoietin-1 increased on 72hr. The present study showed that the microvesselmaturation might to be involved in the expression of EPI and Angiopoietin-1.

内皮细胞和周细胞在血管生成素系统的血管生成中发挥着关键作用。内皮细胞和周细胞交叉（EPI）由周细胞的细胞质投射和相应的内皮凹痕组成，经常出现在血管生成位点。将热可逆凝胶聚合物盘植入大鼠皮下，在体外培养24-72小时。毛细血管密度在48小时时达到峰值。观察到许多EPI在72小时达到峰值。连续切片电子显微镜免疫组织化学分析显示 Angiopoietin-1 定位于周细胞和 EPI。定量RT-PCR分析显示Angiopoietin-1的水平在72小时时增加。本研究表明微血管成熟可能与EPI和Angiopoietin-1的表达有关。

项目成果

Human drug transporter gene-expressing cells are useful alternatives to predictpharmacokinetics in man

人类药物转运蛋白基因表达细胞是预测人类药代动力学的有用替代品

• 发表时间: 2011
• 期刊: AATEX
• 作者: Anzai;N.;Wakui;S.;Jutabha;P.;Muto;T.;Hayashi;M.;Hayashi;K.;Domae;M.,Uchida;K.;Wempe;M.F. and Endou;H
• 通讯作者: H

Invitro thermoreversible gel disc quantitative assay of rat angiogenesis

大鼠血管生成的体外热可逆凝胶盘定量分析

• 发表时间: 2011
• 期刊: AATEX
• 作者: Wakui;S.;et al
• 通讯作者: et al

LAT1 antagonists suppressed N-methyl-N'- nitrosoguanidine induced rat gastric carcinogenesis

LAT1拮抗剂抑制N-甲基-N-亚硝基胍诱导的大鼠胃癌发生

• 发表时间: 2010
• 期刊: J. Toxicol. Pathol
• 作者: Ishida;K.;Muto;T.;Motohashi;M.,Kobayashi;Y.;Imai;K. and Wakui;S
• 通讯作者: S

Mrophometorical analysis on preneoplastic cellular nuclei in BBN induced rat bladder carcinogenesis

BBN诱导大鼠膀胱癌发生前细胞核的形态学分析

• 发表时间: 2012
• 期刊: J. Toxicol. Pathol
• 作者: Wakui;S.;Mtohashi;M.;Imai,K.,Kobayashi;Y.;Tanaka;I.;Nishimoto;T.;Sato;T.;Muto;T.;Takahashi;H. and Hano;H
• 通讯作者: H

Potent hURAT1 inhibitors: in vitro and in vivo metabolism and pharmacokineticstudies

有效的 hURAT1 抑制剂：体外和体内代谢和药代动力学研究

• 发表时间: 2011
• 期刊: Drug Design, Develop, Therapy
• 作者: Wempe;M.F.;Rice;P.J.;Lightner;J.W.;Jutabha;P.;Hayashi;M.;Anzai;N.;Wakui,S.;Kusuhara;H.;Sugiyama;Y. and Endou,H
• 通讯作者: Y. and Endou,H