Three-dimensional ultrastructural study of Endothelial cells and pericytes interdigitation in angiogenesis

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Endothelial cells and pericytes play critical role in angiogenesis by the angiopoietin system. Endothelial cell and pericyte interdigitations (EPI), consisting of cytoplasmic projections of pericytes and corresponding endothelial indentations, are frequently present at angiogenic sites. Rats subcutaneous implantation of a thermoreversible gelation polymer discs were inqubated in vitro 24-72hr. The capillary density increased to a peak on 48hr. A number of EPI were observed to a peak on 72hr. Serial sectioning electron microscopy immunohistochemical analysis revealed that Angiopoietin-1 localized at Pericytes and EPI. Quantitative RT-PCR analysis revealed that the level of Angiopoietin-1 increased on 72hr. The present study showed that the microvesselmaturation might to be involved in the expression of EPI and Angiopoietin-1.