Endothelial Cell-Pericyte Interdigitation in Angiogenesis

血管生成中的内皮细胞-周细胞交叉

• 批准号: 09660332
• 负责人: WAKUI Shin
• 金额: $ 2.18万
• 依托单位: Azabu University School of Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660332/
• 关键词: Angiogenesis; Endothleial cell-pericyte interdigitation; VEGF

In the present study, I have investigated the ultrastructural localization of TGFb, plasminogen activator(PA) and pasminogen(Pn) in granulation tissue. Angiogenesis is a prominent feature of granulation tissue and is largely composed of an abundance of immature capillaries. A number of endothleial cell-pericyte interdigitation(EPI) are observed in immature capillaries in granulation tissue. In this study, TGFb, PA, and Pn immunoreactivities were colocalized selectively at a large number of the EPI in the newly formed capillaries. The present study opens several new directions for the study concerning angiogenesis in situ, especially the heterotypic cell-to-cell interaction(EPI) with angiogenic process.

在本研究中，我研究了TGF β，纤溶酶原激活剂（PA）和纤溶酶原（Pn）在肉芽组织的超微结构定位。血管生成是肉芽组织的显著特征，并且主要由丰富的未成熟毛细血管组成。肉芽组织中未成熟毛细血管内可见大量内皮细胞-周细胞交错排列（EPI）。在这项研究中，TGF β，PA，和Pn免疫反应共定位选择性在大量的EPI在新形成的毛细血管。本研究为原位血管生成的研究开辟了新的方向，特别是异型细胞间相互作用（EPI）与血管生成过程。

项目成果

Mangold,K.et al.: "P16(CDKN2/MTS1)tumor suppressor deletions are present in prostatic carcinoma in Japanese but not Americans" Journal of Urology. 157. 1117-1120 (1997)

Mangold, K. 等人：“日本人的前列腺癌中存在 P16(CDKN2/MTS1) 肿瘤抑制基因缺失，而美国人则不存在”《泌尿学杂志》。

Wakui,S. et al.: "Expression of vascular endothelial growth factor in N-butyl-N-"Veterinary Pathology. 36. 111-116 (1999)

和奎，S.

Takahashi,H., Ichimiya,S., Nimura,Y., Watanabe,M., Furusato,M., Wakui,S., Yatani,R., Aizawa,S., Nakagawa,A.: "Mutation, allelotyping, and transcription analyses of the p73 gene in prostatic carcinoma."Cancer Research. 58. 2076-2077 (1998)

Takahashi,H.、Ichimiya,S.、Nimura,Y.、Watanabe,M.、Furusato,M.、Wakui,S.、Yatani,R.、Aizawa,S.、Nakakawa,A.：“突变、等位基因型、

Takahashi, H.et al.: "Mutation, allelotyping, and transcription analyses of the p73 gene in prostatic..." Cancer Research. 58. 2076-2077 (1998)

Takahashi, H.et al.：“前列腺中 p73 基因的突变、等位基因型和转录分析……”癌症研究。

Wakui,S., Furusato,M., Muto,T., Ohshige,H., Ushigome,S.: "TGF-beta and plasminogen activator at endothelial cell and pericyte interdigitation in humangranulation tissue."Microvascular Research. 54. 262-269 (1997)

Wakui,S.、Furusato,M.、Muto,T.、Ohshige,H.、Ushigome,S.：“人类肉芽组织中内皮细胞和周细胞交叉的 TGF-β 和纤溶酶原激活剂。”微血管研究。