Obstructed Migration of Trigeminal Motoneurons and Trigeminal

三叉神经运动神经元和三叉神经的迁移受阻

• 批准号: 07838048
• 负责人: TERASHIMA Toshio
• 金额: $ 1.6万
• 依托单位: Tokyo Metropolitan Institute for Neuroscience
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07838048/
• 关键词: reeler mouse; trigeminal motor nucleus; trigeminal mesencephalic nucleu; reelin; gill arch; facial nucleus; 細胞移動; ハンマーツ-マウス; 神経奇形マウス; 神経堤; カルボンアニン蛍光色素

Reeler (gene symbol, rl) is an autosomal recessive mutation occurring in mice and characterized by the abnormal positioning of neurons in the central nervous system. In this mutant, however, the cytoarchitecture of the peripheral nervous system is normal, implying that the reeler genetic locus does not affect migration of neurons in the periperal system. Mesencephalic trigeminal nucleus (Me5) neurons are unique in that they are derived from the neural crest like other neurons in the peripheral nervous system, but enter secondarily the central nervous system. We labeled Me5 neurons by injecting horseradish peroxidase or Fast Blue into the temporal muscle of normal and reeler mice to determine whether the migration of these neurons is affected by the reeler genetic locus. Labeled Me5 neurons of the reeler mouse were more widely scattered in comparison with their normal counterparts, suggesting that the reeler genetic locus affects migration of neurons which originate in the neural crest and then migrate into the central nervous system.

Reeler（基因符号，rl）是一种常染色体隐性突变，发生在小鼠身上，其特征是中枢神经系统神经元定位异常。然而，在这个突变体中，周围神经系统的细胞结构是正常的，这意味着reeler基因位点不影响周围系统中神经元的迁移。中脑三叉神经核（Me5）神经元的独特之处在于它们像周围神经系统中的其他神经元一样来源于神经嵴，但其次进入中枢神经系统。我们通过将辣根过氧化物酶或固蓝注射到正常和reeler小鼠的颞肌中来标记Me5神经元，以确定这些神经元的迁移是否受reeler遗传位点的影响。标记的Me5神经元的reeler小鼠更广泛地分散在与他们的正常同行相比，这表明reeler遗传基因座影响迁移的神经元起源于神经嵴，然后迁移到中枢神经系统。

项目成果

Kotani, M., Terashima, T., and Tai, T.: "Developmental changes of genglioside expressions in postnatal rat cerebellar cortex." Brain Research.

Kotani, M.、Terashima, T. 和 Tai, T.：“出生后大鼠小脑皮质中 genglioside 表达的发育变化。”

Terashima, T.: "Anatomy, development and lesion-induced plasticity of rodent corticospinal tract." Neurosci. Res.22. 139-161 (1995)

Terashima, T.：“啮齿动物皮质脊髓束的解剖学、发育和损伤诱导的可塑性。”

Terashima, T.: "The course and collaterals of corticospinal fibers arising from the sensory-motor cortex of the reeler mouse" Devlop. Neurosci.17. 8-19 (1995)

Terashima, T.：“卷轴小鼠感觉运动皮层产生的皮质脊髓纤维的走向和侧支”Devlop。