Mechanisms of Layer Formation of Cerebral Neocortex and Layer-specific Neural Circuits

大脑新皮质层形成机制和层特异性神经回路

• 批准号: 17300111
• 负责人: TERASHIMA Toshio
• 金额: $ 9.58万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300111/
• 关键词: Reelin; Cerebral Cortex; Layer Structure; Reeler; リーラー; 層形成; カルボシアニン; ヨタリ; SRK; 皮質脊髄路; 皮質視床路

Reeler and yotari mice that are mutant for Reelin or Dab1, respectively, show disorders of cerebral cortical lamination. We injected horseradish peroxidase (HRP)into the upper lumbar enlargement to label corticospinal tract (CST) neurons and wheat germ agglutinin-conjugated HRP (WGA-HRP) into the ventrolateral nucleus of the thalamus to label corticothalamic tract (CTT) neurons in both 19-day-old yotari and reeler mice with the aim of discovering whether or not they show differences in the distribution pattern of layer V or layer VI neurons. Similar injections of tracers were made in normal controls. HRP-labeled CST neurons, which were exclusively distributed in the layer V of the normal cortex, were radially scattered in the cortex of both mutants, but those in reeler were more deeply distributed than in yotari. WGA-labeled CTT neurons, which were mainly located in layer VI in the normal cortex, were superficially distributed just beneath the pia mater in both reeler and yotari cortex. The present quantitative study shows that the distribution pattern of layer V neurons, but not layer VI neurons, differs between reeler and yotari mice, suggesting that the Reelin and Dab1 proteins may play different roles in the migration and cell positioning of layer V neurons.

分别为Reelin或Dab 1突变的Reeler和yotari小鼠表现出大脑皮质分层障碍。我们注射辣根过氧化物酶（HRP）到上腰椎扩大标记皮质脊髓束（CST）神经元和小麦胚芽凝集素标记的HRP（WGA-HRP）到丘脑腹外侧核标记皮质丘脑束（CTT）神经元在19日龄yotari和reeler小鼠的目的是发现他们是否表现出不同的分布模式的V层或VI层神经元。在正常对照中进行类似的示踪剂注射。HRP标记的CST神经元，这是专门分布在正常皮层的V层，径向分散在两个突变体的皮层，但在reeler更深的分布比yotari。WGA标记的CTT神经元主要分布在正常皮质的第VI层，在reeler和yotari皮质的软脑膜下表面分布。目前的定量研究表明，第V层神经元的分布模式，但不是第VI层神经元，不同的reeler和yotari小鼠，这表明，Reelin和Dab 1蛋白可能发挥不同的作用，在迁移和细胞定位的第V层神经元。

项目成果

三輪佳宏編 実験がうまくいく蛍光・発光試薬の選び方と使い方 蛍光ニューロントレーサー

编辑：Yoshihiro Miwa 如何选择和使用荧光和发光试剂以实现成功的实验 荧光神经元示踪剂

• 发表时间: 2007
• 作者: 田中靖史;寺島俊雄
• 通讯作者: 寺島俊雄

Origin of climbing fiber neurons and their developmental dependence on Ptf1a

• DOI: 10.1523/jneurosci.1423-07.2007
• 发表时间: 2007-10-10
• 期刊: JOURNAL OF NEUROSCIENCE
• 影响因子: 5.3
• 作者: Yamada, Mayumi;Terao, Mami;Hoshino, Mikio
• 通讯作者: Hoshino, Mikio

Adenovirus-mediated gene transfer in olfactory epithelium and olfactory bulb: A long-term study

• DOI: 10.1177/000348940511400808
• 发表时间: 2005-08-01
• 期刊: ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY
• 影响因子: 1.4
• 作者: Doi, K;Nibu, K;Terashima, T
• 通讯作者: Terashima, T

Expression of zebrafish ROR alpha gene in cerebellar‐like structures

• DOI: 10.1002/dvdy.21275
• 发表时间: 2007-09
• 期刊: Developmental Dynamics
• 影响因子: 2.5
• 作者: Y. Katsuyama;Yoshihiro Oomiya;Hideyuki Dekimoto;Eriko Motooka;Ai Takano;S. Kikkawa;M. Hibi;T. Terashima

The superficial layers of the superior colliculus are cytoarchitectually and myeloarchitectually disorganized in the reelin-deficient mouse, reeler

• DOI: 10.1016/j.brainres.2006.09.113
• 发表时间: 2007-04-06
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Baba, Kousuke;Sakakibara, Shunsuke;Terashima, Toshio
• 通讯作者: Terashima, Toshio