Abnormal migration of the motor trigeminal, facial, and ambiguus nuclei neurons of the SRK rat

SRK 大鼠运动三叉神经元、面部神经核和模糊核神经元的异常迁移

• 批准号: 09832013
• 负责人: TERASHIMA Toshio
• 金额: $ 2.11万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09832013/
• 关键词: reeler; Shaking Rat Kawasaki; facial nucleus; ambiguus nucleus; motor trigeminal nucleus; migration; reelin; 細胞移動; そしゃく; SPKラット

Reeler mouse is an autosomal recessive mutant mouse, and characterized by ataxic gait and tremor. Since reelin, the gene responsible for the reeler mutation is discovered by D'Arcangelo et al. (Nature 374 : 719-723, 1995), much progresses have occurred in this field. The reelin gene encodes an extracellular protein that is crucial for neuronal migration. During the embryogenesis, reelin is expressed in the cerebral cortex in Cajal-Retzius cells and in the cerebellar cortex in outer granule cells. Although non-laminated structures such as facial nucleus, inferior olivary complex, and dorsal cochlear nucleus are also cytoarchtectually deranged in this mutant, only a few studies have been done to clarify the detailed abnormalities in these non-laminated structures. In this review, we focused the cytoarchitectonic abnormality in the facial nucleus of the reeler mouse. The branchiomotor neurons in the facial nucleus are born in the ventricular zone of the floor of the fourth ventricle, migr … More ate ventrolaterally, and finally settle near by the ventral surface of the hindbrain. Time schedules for the generation, axon formation and migration of facial motoneurons are similar both in normal and reeler mouse, but the reeler phenotype becomes identifiable at the end of neuronal migration. Although the reason why the facial nucleus is cytoarchitecually abnormal in the reeler mouse, such a long migration of the facial motoneurons seems to be more susceptible to Reelin in the reeler mouse than in the normal control. In spite of cytoarcitectual abnormality, retrograde horseradish peroxidase (HRP) study confirmed that musculotopic arrangements within the facial nucleus of the reeler mouse are still preserved, suggesting neuronal migration/rearrangement and target recognition are independently regulated.More recently, other kinds of reeler-like mutants have been known (yotari, scrambler, SRK). Among these, yotari and scrambler mice arises from mutations in mdab1, a mouse gene related to the Drosophila gene disabled (dab). More than 10 years ago, an autosomal recessive rat mutant, shaking rat Kawasaki (SRK), has been described that exhibits a phenotype identical to reeler, but the gene responsible for this rat mutation has remained unknown. Very interestingly, the facial nucleus is cytoarchitectually more deranged in yotari and SRK compared to the normal counterparts. The reason why yotari exhibits a phenotype identical to reeler in the laminated structures but not in non-laminates structures such as the facial nucleus has remained obscure, suggesting mDab1 and Reelin proteins function as signaling molecules in a different way between non-laminated and laminated structures. Less

Reeler小鼠是一种常染色体隐性突变小鼠，以共济失调步态和震颤为特征。自Reelin以来，D‘Arcangelo等人发现了导致卷轴突变的基因。(自然374：719-723,1995)，这一领域已经取得了许多进展。Reelin基因编码一种对神经元迁移至关重要的细胞外蛋白。在胚胎发育过程中，Reelin在大脑皮层Cajal-Retzius细胞表达，在小脑皮质外颗粒细胞表达。虽然在该突变体中，面神经核、下橄榄核复合体和耳蜗背核等非层状结构也存在细胞结构错乱，但对这些非层状结构的详细异常研究还很少。在这篇综述中，我们重点介绍了卷轴鼠面神经核中的细胞构筑异常。面神经核内的分支运动神经元出生于第四脑室底部的脑室带，即Migr…。更多的人吃腹侧的食物，最后在后脑的腹面附近安顿下来。正常和卷轴鼠面运动神经元的发生、轴突形成和迁移的时间安排是相似的，但卷轴表型在神经元迁移结束时变得可识别。尽管卷轴鼠面神经核细胞结构异常的原因是，卷轴鼠面运动神经元的如此长时间的迁移似乎比正常对照组更容易受到Reelin的影响。尽管细胞结构异常，逆行辣根过氧化物酶(HRP)研究证实，卷轴鼠面神经核内的肌肉排列仍然保留，表明神经元迁移/重排和目标识别是独立调节的。最近，还发现了其他类型的卷轴样突变体(yotari，scurbler，SRK)。其中，yotari和scurbler小鼠是由mdab1突变产生的，mdab1是一种与果蝇基因失效(Dab)相关的小鼠基因。10多年前，一种常染色体隐性遗传的大鼠突变体--摇晃大鼠川崎(SRK)被描述为表现出与Reeler相同的表型，但导致这种大鼠突变的基因至今仍不清楚。非常有趣的是，与正常的对应者相比，yotari和srk的面神经核在细胞结构上更加错乱。Yotari在层叠结构中表现出与Reeler相同的表型，而在非层叠结构(如面神经核)中表现出与Reeler相同的表型，其原因尚不清楚，这表明mDab1和Reelin蛋白在非层叠结构和层叠结构中作为信号分子的功能不同。较少

项目成果

Ikeda, K: "Corticospinal tract neurons are radially malpositioned in the sensory-motor cortex of the shaking Rat Kawasaki"J. Comp. Neurol.. 382. 1-11 (1997)

Ikeda, K：“在川崎大鼠的感觉运动皮层中，皮质脊髓束神经元呈放射状错位”J.

Fujimoto,Y., Setsu,T., Ikeda,Y., Miwa,A., Okado,H., and Terashima,T.: "Ambiguus nucleus neurons innervating the abdominal esophagus are malpositioned in the reeler mouse"Brain Res.. 811. 156-160 (1998)

Fujimoto,Y.、Setsu,T.、Ikeda,Y.、Miwa,A.、Okado,H. 和 Terashima,T.：“支配腹部食管的模糊核神经元在 reeler 小鼠中错位”Brain Res..

Terashima,T.: "Retrograde and anterograde labeling of cerebellar afferent projection by the injection of recombinant adenoviral vectors into the mouse cerebellar cortex" Anatomy and Embryology. 196. 363-382 (1997)

Terashima,T.：“通过将重组腺病毒载体注射到小鼠小脑皮质中来逆行和顺行标记小脑传入投射”解剖学和胚胎学。

寺島俊雄: "脳と神経-分子神経生物学入門共立出版"金子章道、川村光毅、植村慶一編. 17 (1999)

Toshio Terajima：“大脑和神经 - 分子神经生物学概论 Kyoritsu Shuppan”由 Akimichi Kaneko、Mitsuki Kawamura 和 Keiichi Uemura 编辑 17 (1999)。

寺島俊雄: "リーラーマウス顔面神経核の細胞構築異常"解剖学雑誌. 74(4). 411-420 (1999)

Toshio Terashima：“Reeler 小鼠面神经核的细胞结构异常”解剖学杂志 74(4) (1999)。