A study on proteoglycans which have a protective action against neuronal death

观察了硫酸软骨素蛋白多糖(CSPGs)对兴奋性氨基酸诱导的原代培养大鼠脑神经元延迟性死亡的影响。1)新生SD大鼠脑内可溶性部分CSPGs呈剂量依赖性地保护兴奋性氨基酸诱导的脑神经元和海马神经元延迟性死亡。通过检测CSPGS核心蛋白的CNBR多肽对受体酪氨酸磷酸酶Zeta/beta的胞外变体Checroitin硫酸蛋白多糖的影响。3)与假定的磷酸核心蛋白区域同源的重组蛋白对谷氨酸诱导的海马神经元延迟性死亡具有保护作用。结果表明，磷酸核心蛋白的一个特定区域对Barin缺氧/缺血引起的神经元死亡具有神经保护作用。4)利用抗脂蛋白脂酶多克隆抗体建立了检测PC12细胞培养上清液和大鼠脑标本中硫酸乙酰肝素蛋白多糖的酶联免疫吸附分析方法。

The effects of chondroitin sulfate proteoglycans (CSPGs) on delayd neuronal death induced by excitatory amino acids were examined in primary cartured rat cerebral neurons.1) CSPGs perepared from the soluble fraction of neonatal SD rat brain protected delayd cell death of both cerebral and hippocampal neurons induced by excitatory amino acids in a dose-dependent manner.2) This protective action was suggeted to be due to phosphacan, a chodroitin sulfate proteoglycan which is a extracellular variant of receptor-type protein tyrosine phosphatase zeta/beta by examining effects of CNBr peptides of CSPGs core protein.3) Recombinant proteins homologous to the supposed core protein region of phosphacan protected delayd cell death of hippocampal neurons induced by glutamate.The results indicated that a specific region of phosphacan core protein had neuroprotective action against neuronal death due to anoxia/ischemia of barin.4) An enzyme-linked immunosorbent assay, utilizing anti-lipoprotein lipase polyclonal antiboies, for measurement of heparan sulfate proteoglycan was established, which could detect nanomolar level o heparan sulfate in samples prepared from PC12 cell culture medium and rat brain.