Bone formation by the implantation of bone marrow cells

通过骨髓细胞的植入形成骨

• 批准号: 07671283
• 负责人: MIZUNO Morimichi
• 金额: $ 1.41万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671283/
• 关键词: Osteoblasts; Bone; Bone marrow cells; Collagen gel

In this study, we attempted to form a mass of bone by the implantation of osteoblastic cells differentiated from bone marrow stromal cells. Dexamethasone or vitamin C have been utilized to induce the osteoblastic differentiation so far, however, there is no report that these cells formed bone in vivo. Recently we found that collagen gel induced the osteoblastic differentiation without dexamethasone or vitamin C.These cells with collagen gel induced bone and a volume of bone increased time-dependently in vivo. On the other hand, un-differentiated bone marrow cells with collagen gel did not form bone. These results indicate that bone marrow stromal cells which expressed the osteoblastic phenotypes could form bone effectively in vivo. The effect of collagen gel is specific for type I,and type II-V collagen did no? possess the inducing activity. Furthermore, TGF-beta enhanced the osteoblastic differentiation by collagen gel. Our results demonstrate that bone marrow cells could form a mass of bone by our procedure. The benefit of our method is 1) we do not use dexamethasone, which has a potent side-effect, 2) we utilized collagen, which shows affinity to tissues.

在这项研究中，我们试图通过移植从骨髓基质细胞分化的成骨细胞来形成骨块。地塞米松或维生素C已被用于诱导成骨细胞分化，但尚未见这些细胞在体内成骨的报道。最近我们发现，在不使用地塞米松或维生素c的情况下，胶原凝胶诱导成骨细胞分化，这些细胞在体内具有时间依赖性，骨体积增加。另一方面，未分化的骨髓细胞与胶原凝胶不形成骨。这些结果表明，表达成骨表型的骨髓基质细胞在体内可以有效地成骨。胶原凝胶的作用对I型有特异性，而II-V型胶原没有？具有诱导活性。tgf - β通过胶原凝胶促进成骨细胞分化。我们的结果表明，骨髓细胞可以通过我们的程序形成大量的骨。我们的方法的好处是：1)我们不使用地塞米松，它有强烈的副作用；2)我们使用胶原蛋白，它对组织有亲和力。

项目成果

Mizuno M.: "The osteoblaobic MC3T3-E1 cellssynthesized C-berminal propaptide of type I Cellayen,which promoted all-attachment of osteohlasts," Biochemica et Biophysica Acta. (in press). (1995)

Mizuno M.：“骨细胞 MC3T3-E1 细胞合成了 I 型 Cellayen 的 C-berminal propaptide，它促进了成骨细胞的全附着”，《生物化学与生物物理学学报》。

Mizuno M.: "Bone chondroad herin promobes allachment of osbeoblastic cells to solid-stabe subsbrates and shows affiniby to collagen" Calcified Tissne Inbernabional. 59. 163-167 (1996)

Mizuno M.：“骨软骨软骨素促进成骨细胞与固体基质的附着，并显示出与胶原蛋白的亲和力”钙化 Tissne Inbernabional。

Mizuno, M., Fujisawa, F., Kuboki, Y.: "Bone chondroadherin promotes attachment of osteoblastic cells to solid-state substrates and affinity to collagen." Calcified Tissue International. 59. 163-167 (1996)

Mizuno, M.、Fujisawa, F.、Kuboki, Y.：“骨软骨粘合素促进成骨细胞与固态基质的附着以及与胶原蛋白的亲和力。”

Mizuno M.: "The osteoblastic MC3T3-EI cells synthesized C-terminal propeptide of type T collagen, which promoted cell-attachment of ostesblasts" Biochemica et Biophysica Acta. 1310. 97-102 (1996)

Mizuno M.：“成骨细胞 MC3T3-EI 细胞合成了 T 型胶原蛋白的 C 末端前肽，促进了成骨细胞的细胞附着”Biochemica et Biophysical Acta。

Mizuno M.: "The osteoblastic MC3T3-E1 cells synthesized C-terminal propeptide of type I collagen, which promoted cell-atbachment of osfeoblasi" Biochenica et Biophysica Acba. 1310. 97-102 (1996)

Mizuno M.：“成骨细胞 MC3T3-E1 细胞合成了 I 型胶原蛋白的 C 端前肽，促进了成骨细胞的细胞附着”Biochenica et Biophysicala Acba。