Regeneration of mandibular bone by the implantation of cell fixed artificial periostium

细胞固定人工骨膜植入实现下颌骨再生

• 批准号: 13557186
• 负责人: MIZUNO Morimichi
• 金额: $ 8.51万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557186/
• 关键词: artificial periostium; osteoblastic differentiation; bone formation; 顎骨; 再生; 骨芽細胞; 骨髄細胞; コラーゲン

So far, the injection of growth factor, and implantation of cells have been attempted to regenerate bone ; however, the bone mass formed by these treatments is little. In this study, I tried to develop new artificial periostium constituted by cells, growth factors and carrier, and investigated the optimal condition for the regeneration of bone in aged peoples. First of all, I looked for the factors that the artificial periostium should have. I harvested bone marrow cells to fix into artificial periostium. They possess the ability to differentiate into osteoblasts, and this differentiation is induced by the treatment of cells by dexamethasone, but dexamethasone is a kind of hormone, and is hard to use the clinical treatment. It has been reported that type I collagen has an ability to induce the osteobrastic differentiation of bone marrow cells, and I studied whether type I collagen induced this kind of differentiation. Osteocalcin and bone sialoprotein gene are osteoblast-specific one, and I found that bone marrow cells cultured in type I collagen gel showed high expression of these genes. Next I implanted the artificial periostium in rats, and found it formed bone in vivo. These results indicate that the artificial periostum we constructed has an ability to form bone, and I clould conclude that the artificial periostum is a potent tool to regenerate the mandibular bone.

迄今为止，已尝试注射生长因子和植入细胞来再生骨;然而，通过这些治疗形成的骨量很少。本研究试图研制一种由细胞、生长因子和载体组成的新型人工骨膜，并探讨老年人骨再生的最佳条件。首先，我寻找人工骨膜应该具备的因素。我采集骨髓细胞植入人造骨膜。它们具有向成骨细胞分化的能力，这种分化是通过地塞米松处理细胞来诱导的，但地塞米松是一种激素，很难用于临床治疗。据报道，I型胶原具有诱导骨髓细胞向成骨细胞分化的能力，我研究了I型胶原是否诱导了这种分化。骨钙素和骨唾液酸蛋白基因是成骨细胞特异性基因，我发现在I型胶原凝胶中培养的骨髓细胞显示这些基因的高表达。接下来，我将人工骨膜植入大鼠体内，发现它在体内形成了骨。这些结果表明，我们构建的人工骨膜具有成骨能力，是一种有效的下颌骨再生工具。

项目成果

Mizuno M.: "Type I Collagen Regulated Dentin Matrix Protein I and Osteocalcin Gene Expression of Dental Pulp Cells"Journal of Cellular Biochemistry. (in press). (2003)

Mizuno M.：“I型胶原调节牙本质基质蛋白I和牙髓细胞的骨钙素基因表达”细胞生物化学杂志。

Ojima Y.: "In Vitro Effect of Platelet-derived Growth Factor-BB on Collagen Synthesis and Proliferation of Human Periodontal Ligament Cells"Oral Desease. (in press). (2003)

Ojima Y.：“血小板衍生生长因子-BB 对胶原合成和人牙周膜细胞增殖的体外影响”口腔疾病。

Mizuno, M: "Type I Collagen Regulated Dentin Matrix Protein 1 and Osteocalcin Gene Expression of Rat Dental Pulp Cells"Journal of Cellular Biochemistry. 88. 1112-1119 (2003)

Mizuno，M：“I型胶原调节大鼠牙髓细胞的牙本质基质蛋白1和骨钙素基因表达”细胞生物化学杂志。

Ojima Y.: "In vitro effect of platelet-derived growth factor-BB on collagen synthesis and proliferation of human periodontal ligament cells"Oral Disease. (In press). (2003)

Ojima Y.：“血小板衍生生长因子-BB 对人牙周膜细胞胶原蛋白合成和增殖的体外影响”口腔疾病。

Mizuno M.: "Type I collagen regulated dentin matrix protein 1 and osteocalcin gene of rat dental pulp cells"Journal of Cellular Biochemistry. 88. 1112-1119 (2003)

Mizuno M.：“I型胶原调节大鼠牙髓细胞的牙本质基质蛋白1和骨钙素基因”细胞生物化学杂志。