权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Regeneration of tooth supporting tissue by the implantation of cell fixed collagen membrane

通过植入细胞固定胶原膜实现牙齿支持组织的再生

基本信息

批准号：
12672027
负责人：
MIZUNO Morimichi
金额：
$ 2.05万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672027/
关键词：
Periodonhtal tissue Regenerating Osteoblasts Bone marrow stem cells Collagen bFGF BMP

项目摘要

The aim of this study is to promote the regeneration of tooth supporting tissue by the implantation of'cell fixed collagen membrane. The crucial stage in this study is creation of cell fixed membrane, and I first investigated features of cells to satisfy the regeneration of tooth supporting tissue by the collagen membrane. The stem cells harvested from bone marrow were fixed in the collagen membrane, and the membrane was cultured for four weeks to analyze bone forming activity. The bone forming activity was estimated by the measurement of alkaline phosphatase activity and detection of osteoblast-specific gene (osteocalcin, bone sialoprotein), and I recognized bone forming activity in the collagen membrane. To promote the bone forming activity, Bone Morphogenetic Protein (BMP), of basic Fibroblast Growth Factor (bFGF) was added in the membrane. When BMP was mixed in the collagen membrane, the bone forming activity appeared faster. However, the intensity of bone forming activity did not change by the addition of BMP. This might be due to the enhancement of osteoblastio differentiation by BMP. Next I examined the effect of bFGF. The total bone forming activity of the membrane increased when high dose of bFGF was mixed in the collagen membrane. However the bone forming activity of each cell decreased. This finding might indicate that bFGF accelerated cell growth, but suppressed the expression of osteoblastic phenotype. When low dose of bFGF was mixed in the collagen membrane, the bone forming activity of membrane increased. However this activation did not occurred if bFGF was not added in the culture medium. This might be due to the loss of bFGFfrom the collagen membrane. I am going to create the new cell fixed membrane which could maintain the low dose of bFGF for a long time.

本研究的目的是通过细胞固定胶原膜的植入促进牙支撑组织的再生。本研究的关键阶段是细胞固定膜的制备，我首先研究了细胞的特性，以满足胶原膜对牙齿支撑组织的再生。将骨髓干细胞固定在胶原膜中，培养4周，分析成骨活性。通过测定碱性磷酸酶活性和检测成骨细胞特异性基因（骨钙素、骨唾液蛋白）来估计成骨活性，并在胶原膜中识别出成骨活性。为了促进骨形成活性，在膜中加入碱性成纤维细胞生长因子（bFGF）的骨形态发生蛋白（BMP）。胶原膜中掺入BMP后，骨形成活性加快。然而，骨形成活性的强度并没有因BMP的加入而改变。这可能是由于BMP增强了成骨细胞分化。接下来，我检查了bFGF的效果。在胶原膜中掺入高剂量bFGF后，膜的总成骨活性增加。然而，各细胞的成骨活性下降。这一发现可能表明bFGF加速细胞生长，但抑制成骨细胞表型的表达。当低剂量bFGF掺入胶原膜时，膜的成骨活性增加。然而，如果培养基中不添加bFGF，则不会发生这种激活。这可能是由于胶原膜中bfgf的损失。我将创造一种新的细胞固定膜，它可以长时间维持低剂量的bFGF。