Experimental Study on the Carriers for Osteo- and Chondro-induction by Bone Mophogenetic Protein.

骨形态发生蛋白诱导骨和软骨载体的实验研究。

• 批准号: 07671343
• 负责人: KUSUMOTO Kenji
• 金额: $ 1.54万
• 依托单位: Kansai Medical University, Faculty of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671343/
• 关键词: Bone morphogenetic protein; osteoinduction; carrier; atelopeptide type I collagen; osteogenesis; skeletal reconstruction; 骨形式因子; 骨形成因子(BMP); コラーゲン; ハイドロキシアパタイト

Atelopeptide type I collagen (CL) and porous hydroxyapatite (pHAP) were used as a carrier for heterotopic osteoinduction by BMPs, purified BMP,rhBMP-2 and rxBMP-4/7 heterodimer.In using CL,purified BMP and rhBMP-2 osteoinduced ectopic bone in rat calf muscle (Kusumoto et al.BBRC 1995). However, massive trabeculum in purified BMP groups and massive marrow in rhBMP-2 groups were induced.In using CL and pHAP,rhBMP-2 induced ectopicly in rat calf muscle (Kusumoto et al.J Cranio-Maxillofac Surg 1996). Trabeculum was induced in the pores of pHAP,even in the deep pores, and the amount was dependent upon the dosage of rhBMP-2.In using CL,rhBMP-2 and rxBMP-4/7 heterodimer induced ectopic bone (Kusumoto et al.BBRC 1997). The amount of trabeculum in rhBMP-2 group was more than in rxBMP-4/7 heterodimer. Osteoinducing ability in rhBMP-2 gorup was vigorous than in rxBMP-4/7 heterodimer group.

将末端肽I型胶原（CL）和多孔羟基磷灰石（pHAP）用作通过BMP、纯化的BMP、rhBMP-2和rxBMP-4/7异源二聚体进行异位骨诱导的载体。在使用CL和pHAP时，rhBMP-2在大鼠小腿肌肉中诱导异位（Kusumoto等，J Cranio-Maxillofac Surg 1996）。在pHAP的孔中，甚至在深孔中诱导小梁，并且量取决于rhBMP-2的剂量。在使用CL时，rhBMP-2和rxBMP-4/7异二聚体诱导异位骨（Kusumoto等，BBRC 1997）。rhBMP-2组骨小梁数量多于rxBMP-4/7异源二聚体组。rhBMP-2组的成骨能力强于rxBMP-4/7异源二聚体组。

项目成果

Yoshida K,Bessho K,Fujimura K,Kusumoto K,Ogawa Y,Tani Y,Iizuka T: "「Osteoinducing ability by recombinant human bone morphogenetic protein-2 in intramuscular and subcutaneous sites」" J Cranio-Maxillofacial Surg. (in press.).

Yoshida K、Bessho K、Fujimura K、Kusumoto K、Okawa Y、Tani Y、Iizuka T：“重组人骨形态发生蛋白 2 在肌内和皮下部位的骨诱导能力”J Cranio-Maxillofacial Surg。

Kusumoto K, Bessho K, Fujimura K, Konishi Y, Ogawa Y, Iizuka T: "Comparative study of bone marrow induced by purified BMP and recombinant human BMP." Biochem Biophys Res Commun. 215. 205-211 (1995)

Kusumoto K、Bessho K、Fujimura K、Konishi Y、Okawa Y、Iizuka T：“纯化 BMP 和重组人 BMP 诱导骨髓的比较研究。”

Kusumoto K,Bessho K,Fujimura K,Konishi Y,Ogawa Y,Iizuka T: "「Self-regenerating bone implant : Ectopic osteoinduction following intramuscular implantation of a combination of rhBMP-2, atelopeptide type I collagen and porous hydroxyapatite」" J Cranio-Maxill

Kusumoto K、Bessho K、Fujimura K、Konishi Y、Okawa Y、Iizuka T：“‘自我再生骨植入物：肌肉内植入 rhBMP-2、去端肽 I 型胶原蛋白和多孔羟基磷灰石组合后的异位骨诱导’” J Cranio -麦克西尔

別所 和久、藤村 和麿、楠本 健司、飯塚 忠彦: "体液性因子による骨・血管相互作用 BMPファミリーとTGF-βファミリー" Clinical Calcium. 7. 1190-1194 (1997)

Kazuhisa Bessho、Kazumaro Fujimura、Kenji Kusumoto、Tadahiko Iizuka：“体液因素引起的骨血管相互作用：BMP 家族和 TGF-β 家族”临床钙。 7. 1190-1194 (1997)

Kusumoto K, Bessho K, Fujimura K, Ogawa Y, Iizuka T: "Intramuscular osteoinduction and bone marrow formation by the implantation of rhBMP-2 with atelopeptide type I collagen" Brit J Oral Maxillofac Surg. 35(6). 433-437 (1997)

Kusumoto K、Bessho K、Fujimura K、Okawa Y、Iizuka T：“通过植入 rhBMP-2 和去端肽 I 型胶原蛋白进行肌内骨诱导和骨髓形成”Brit J Oral Maxillofac Surg。