Experimental Study of the Promoting Agents for Osteo- and Chondro- induction by Bone Morphogenetic Protein

骨形态发生蛋白骨软骨诱导促进剂的实验研究

• 批准号: 10671691
• 负责人: KUSUMOTO Kenji
• 金额: $ 2.05万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671691/
• 关键词: Bone morphogenetic protein; osteoinduction; carrier; atelopeptide type I collagen; osteogenesis; skeletal reconstruction; 骨再建; アテロペプタイドタイプ I コラーゲン; アテロペプタイドタイプIコラーゲン

Heterotopic osteoinduction ability of bone morphogenetic protein (BMP) is much anticipated for skeletal reconstruction. Concerning the clinical application of BMP, various promoting agents on the osteoinduction by rhBMP-2 were studied. Those agents were prostaglandin E1, E2, cAMP, calcitonine, K2, O2 and blood supply.The phalmachological dosage of these agents promoted the osteoinduction. However, high dosage of the agents suppressed the osteogenesis. Those studies showed that each agent acted on the various phases of osteogenesis.These results suggested adequate dosages of agents in clinical usage of BMP.

骨形态发生蛋白（BMP）的异位诱导成骨能力在骨重建中具有重要的应用价值。在BMP的临床应用方面，研究了各种促进剂对rhBMP-2诱导成骨的影响。这些药物包括前列腺素E_1、E_2、cAMP、降钙素、K_2、O_2和血供，这些药物的剂量对骨诱导有促进作用。但高剂量的药物抑制成骨。这些研究表明，各制剂在成骨的各个阶段都有作用，提示临床应用BMP时应选用适当的制剂剂量。

项目成果

Bessho K,Kusumoto K,Fujimura K,Konishi Y,Ogawa Y,Iizuka T: "Comparison of recombinant and purified human bone morphogenetic protein." Brit J Oral Maxillofac Surg. (in press.).

Bessho K、Kusumoto K、Fujimura K、Konishi Y、Okawa Y、Iizuka T：“重组和纯化的人骨形态发生蛋白的比较。”

Okubo Y, Bessho K, Fujimura K, Konishi Y, Kusumoto K, Ogawa Y, Iizuka T: "Osteogenesis by recombinant human bone morphogenetic protein-2 at skeletal sites."Clinic Orthop Relat Res. (in press.).

Okubo Y、Bessho K、Fujimura K、Konishi Y、Kusumoto K、Okawa Y、Iizuka T：“重组人骨形态发生蛋白 2 在骨骼部位的成骨作用。”Clinic Orthop Relat Res。

Kusumoto K, Bessho K, Fujimura K, Akioka J, Oguwa Y, Iizuka T: "Prefabricated muscle flap including bone induced by recombinant human BMP-2 ; an experimental study of ectopic osteoinduction in a rat latissimus dorsi muscle flap"Brit J Plast Surg. 51. 275-

Kusumoto K、Bessho K、Fujimura K、Akioka J、Oguwa Y、Iizuka T：“重组人 BMP-2 诱导的包含骨的预制肌肉瓣；大鼠背阔肌肌瓣异位骨诱导的实验研究”Brit J Plast Surg

Kiyozuka Y, Miyazaki H, Yoshizawa K, Senzaki H, Yamamoto D, Inoue K, Bessho K, Okubo Y, Kusumoto K, Tsubura A: "An autopsy case of malignant mesothelioma with osseous and cartilagious differentiation : Bone morphogenetic protein-2 in mesothelial cells and

Kiyozuka Y、Miyazaki H、Yoshizawa K、Senzaki H、Yamamoto D、Inoue K、Bessho K、Okubo Y、Kusumoto K、Tsubura A：“具有骨和软骨分化的恶性间皮瘤尸检病例：间皮细胞中的骨形态发生蛋白-2

Kusumoto K, Bessho K, Fujimura K, Akioka J, Okubo Y, Ogawa Y, Iizuka T: "Prefabricated muscle flap with bony tissue ; an experiemental study of heterotopic osteoinduction by a combination of rhBMP-2, cillagen and porous hydroxyapatite wrapped with a muscl

Kusumoto K、Bessho K、Fujimura K、Akioka J、Okubo Y、Okawa Y、Iizuka T：“带骨组织的预制肌肉瓣；通过包有 rhBMP-2、胶原蛋白和多孔羟基磷灰石的组合进行异位骨诱导的实验研究