Experimental study for ectopic osteoinduction with BMP and bone marrow induction

BMP异位骨诱导与骨髓诱导的实验研究

• 批准号: 12671757
• 负责人: KUSUMOTO Kenji
• 金额: $ 2.11万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671757/
• 关键词: Bone Morphogenetic Protein; Osteoinduction; Bone Formation; Skeletal Reconstruction; Tissue Engineering; Cytokine; Growth Factor; Bone Marrow; 骨; Tissue Engineering; 血管新生; 骨形成タンパク(BMP); 異所性骨誘導; 骨新生; 血管

Osteoinduction with BMP (Bone Morphogenetic Protein) in the muscle pocket is the research model, us* recombinant human BMP. In this research, BMP has produced not only trabeculae, but also bone marrow a* angioid tissue. In various conditions, osteoinduction has been investigated.1) BMP expression is confirmed in the course of osteoinduction. (2000-2002)2) Osteoinduction with rhBMP-2 can be produced in the muscle of Japanese monkey (Macaca Fuscata) (2002)3) In the muscle flap implanted rhBMP-2, osteoinduction is confirmed and vascular formation and axalization * investigated using angiogram and histological examination. (2000-2002)4) rhBMP-2 with bFGF promoted osteoinduction. (2002)5) FK506 affects the osteoinduction course with rhBMP-2. (2002)

本研究采用重组人骨形态发生蛋白（BMP）在肌袋内诱导成骨。在本研究中，BMP不仅产生了骨小梁，而且还产生了骨髓血管样组织。在不同的条件下，骨诱导已被调查。1）BMP的表达被证实在骨诱导的过程中。（2000-2002）2）在日本猴（Macaca Fuscata）的肌肉中可以产生rhBMP-2的骨诱导（2002）3）在植入rhBMP-2的肌瓣中，证实了骨诱导，并且使用血管造影和组织学检查研究了血管形成和轴化 *。（2000-2002）4）rhBMP-2联合bFGF促进成骨诱导。（2002）5）FK 506影响rhBMP-2的骨诱导过程。（2002年）

项目成果

Kusumoto K,Bessho K,Fujimura K,Akioka J,Okubo Y,Ogawa Y,Iizuka T: "The effect of blood supply in a muscle and an elevated muscle flap on an endogenous tissue engineered bone by rhBMP-2 in the rat"Ann Plast Surg. 45. 408-414 (2000)

Kusumoto K、Bessho K、Fujimura K、Akioka J、Okubo Y、Okawa Y、Iizuka T：“肌肉中的血液供应和升高的肌肉瓣对大鼠中 rhBMP-2 内源性组织工程骨的影响”Ann

Kaihara S, Bessho K, Okubo Y, Sonobe J, Kusumoto K, Ogawa Y, Iizuka T: "Effect of FK506 on osteoinduction by recombinant human bone morphogenetic protein-2"Life Sciences. 72. 247-256 (2002)

Kaihara S、Bessho K、Okubo Y、Sonobe J、Kusumoto K、Okawa Y、Iizuka T：“FK506 对重组人骨形态发生蛋白 2 骨诱导的影响”生命科学。

Kusumoto K and Bessho K: "Jaw reconstruction with BMP, collagen and hydroxyapatite in vivo"R.G.Landes company, Austin, Texas. (in press).

Kusumoto K 和 Bessho K：“体内用 BMP、胶原蛋白和羟基磷灰石进行颌骨重建”R.G.Landes 公司，德克萨斯州奥斯汀。

Kusumoto K and Bessho K: "Jaw reconstruction with BMP, collagen and hydroxyapatite"the World Scientific Publishing Co., Pte. Ltd.,. 307-328 (2002)

Kusumoto K 和 Bessho K：“用 BMP、胶原蛋白和羟基磷灰石进行下颌重建”，世界科学出版有限公司，Pte。

Okubo Y, Bessho K, Fujimura K, Kusumoto K, Ogawa Y, Iizuka T: "Expression of bone morphogenetic protein in the course of osteoinduction by recombinant human bone morphogenetic protein-2"Clin Oral Impl Res. 13. 80-85 (2002)

Okubo Y、Bessho K、Fujimura K、Kusumoto K、Okawa Y、Iizuka T：“重组人骨形态发生蛋白-2在骨诱导过程中骨形态发生蛋白的表达”Clin Oral Impl Res。