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INNERVATION OF THE BILIARY DUCTS IN HUMANS,PIGS,MONKEYS,DOGS AND HUMSTERS.

人、猪、猴、狗和仓鼠胆管的神经支配。

基本信息

批准号：
07671385
负责人：
KINOSHITA Takashi
金额：
$ 1.15万
依托单位：
Shiga University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671385/
关键词：
biliary ducts nitric oxide synthase NADPH-diaphorase protein gene product 9.5 vasoactive intestinal peptide neuropeptide Y histochemistry protein gene product9.5 vasoactive intestinal peptide neuropeptide Y

项目摘要

The autonomic nervous system appears to play an important role in the biliary ducts, as well as in the digestive tract. In this study, the distribution of nerves in various parts of the biliary ducts were examined in detail using immunohistochemistry and enzymehistochemistry in humans, pigs, monkeys, dogs and humsters. Numerous immunoreactive nerves were seen with antisera against peripheral nerve markers (protein gene product 9.5). These nerves were observed everywhere in the biliary ducts including the hepatic ducts, common bile duct, gallbladder, cystic duct, and the sphincter of the duodenal papilla. In the biliary ducts, acetylcholinesterase (AChE) positive nerve fibers, nitric oxide synthase (NOS) containing nerve fibers, and 5 types of peptidergic nerve fibers including neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), substance P (SP), calcitonin gene-related peptide (CGRP) and enkephalin (EK) were detected. AChE positive nerve fibers, NOS containing nerve fibers and NPY-immunoreactive nerve fibers were abundant, but the other nerve fibers were scarce. Further more, AChE positive nerve cells and NOS containing nerve colls were scattered throughout the biliary ducts. The result of this study suggests that various types of nerves mediate the maintenance of functional states in the biliary ducts, including the transport, storage and excretion of bile juice.

自主神经系统似乎在胆管和消化道中起着重要的作用。本研究采用免疫组织化学和酶组织化学方法对人、猪、猴、狗和仓鼠胆管各部位神经的分布进行了详细的研究。周围神经标记物（蛋白基因产物9.5）的抗血清中可见大量免疫反应神经。胆管内包括肝管、胆总管、胆囊、胆囊管、十二指肠乳头括约肌均可见到这些神经。在胆管中检测到乙酰胆碱酯酶（AChE）阳性的神经纤维、含一氧化氮合酶（NOS）的神经纤维，以及神经肽Y （NPY）、血管活性肠多肽（VIP）、P物质（SP）、降钙素基因相关肽（CGRP）、脑啡肽（EK）等5种能肽神经纤维。AChE阳性神经纤维、含NOS神经纤维和npy免疫反应神经纤维丰富，其他神经纤维较少。胆管内可见AChE阳性神经细胞和含NOS的神经细胞。本研究结果表明，多种类型的神经介导了胆管功能状态的维持，包括胆汁液的运输、储存和排泄。