Microsatellite instability and oncogene expression in gastric cancer

胃癌中微卫星不稳定性和癌基因表达

• 批准号: 07671417
• 负责人: YAMADA Yukishige
• 金额: $ 1.41万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671417/
• 关键词: gastric cancer; oncogene; Microsatellite instability; c-erbB-2; c-met; cyclin E; Replication error; がん抑制遺伝子; マイコロサテライトインスタビリティー; マイクロサテライトインスタビリティー

Overexpression of cyclin E was obserbed in 22.4% of gastric cancer cases. Those were significantly corraleted with depth of invasion and lymph node metastasis. The survival rate of the patients with cyclin E overexpression was significantly poorer than that of the patients without cyclin E overexpression.Overexpression of c-met and c-erbB-2 was obserbed in 46.1% cases and 16.4% cases, respectively. Gene amplification of c-met and c-erbB-2 was detected in 10.2% and 11.7% of gastric cancer cases, respectively. Amplification and overexpression of c-met was significantly correlated with depth of invasion and lymph node metastasis, while amplification and overexpression of c-erbB-2 was significantly correlated with histologic differentiation. The survival rate of the patients with c-met or c-erbB-2 amplification and/or overexpression was significantly poorer than that of the patients without amplification or overexpression.We examined DNA from 42 surgical specimens of gastric cancers for genetic instability at three microsatellite marker loci on chromosomes 2 and 3. We detected differences between tumor and normal DNA banding patterns in 12 of the 42 tumors examined (28.6%). There was no correlation between microsatellite instability and clinicopathological features.

22.4%的胃癌患者中cyclin E过表达。这些与浸润深度和淋巴结转移有显著相关。cyclin E过表达患者的生存率明显低于cyclin E未过表达患者。c-met过表达46.1%，c-erbB-2过表达16.4%。c-met和c-erbB-2基因扩增分别在10.2%和11.7%的胃癌病例中检测到。c-met扩增和过表达与浸润深度及淋巴结转移显著相关，c-erbB-2扩增和过表达与组织学分化显著相关。c-met或c-erbB-2扩增和/或过表达患者的生存率明显低于未扩增或过表达的患者。我们从42例胃癌手术标本中检测了2号和3号染色体上三个微卫星标记位点的遗传不稳定性。我们在42例肿瘤中检测到12例肿瘤与正常DNA带型之间的差异（28.6%）。微卫星不稳定性与临床病理特征无相关性。

项目成果

阪口 晃行: "胃癌におけるCyclinE蛋白発現の検討" 消化器癌の発生と進展. 9. 99-102 (1997)

Akiyuki Sakaguchi：“胃癌中CyclinE蛋白表达的检查”胃肠癌的发生和进展（1997）。

山田 行重: "家族集積の胃癌とMicrosatellite Instabilityについて" 日本外科系連合学会誌. 22. 708-711 (1997)

Yukishige Yamada：“关于胃癌的家族聚集性和微卫星不稳定性”日本外科联合会杂志 22. 708-711 (1997)。