Experimental therapeutics for gastric cancer using adenovirus vectors

1)反义EGFR和反义c-met腺病毒在胃癌细胞中表达EGFR和c-met后，用免疫细胞学方法观察EGFR和c-met反义RNA表达腺病毒(Ad-EAS或Ad-MAS)后，癌细胞表面Eger和c-met蛋白的表达水平分别显著降低。2)Ad-EAS病毒对胃癌细胞体外和活体生长的影响。经EGFR反义RNA表达的腺病毒(Ad-EAS)感染后，Ad-EAS感染细胞的体外生长较对照细胞(AGS、KKLS、MKN28)明显受到抑制(p(]SY.di取代右)。在裸鼠皮下肿瘤系统中，经Ad-EAS治疗后，MKN28的体内肿瘤生长明显受到抑制，第48天的抑制率为93%。3)Ad-MAS病毒对胃癌细胞运动能力的影响：Ad-MAS病毒感染后，AGS(c-met表达)和Hs746t(c-met过表达)的细胞运动能力明显受到抑制。AGS和Hs746T的抑制率分别为88%和45%。在不表达c-met的KKLS细胞中未观察到这些运动抑制。

1) EGER and c-met expression after EGFR and c-met antisense expressing adenovirus in gastric cancer cellsFollowing infection with EGFR or c-met antisense RNA-expressing adenovirus(Ad-EAS or Ad-MAS), the cell surface EGER or c-met protein levels of infected cancer cell were observed by immunocytological method resulting in that markedly reduction of these proteins respectively. These suppression was not observed in non-infection or beta-galactosidase expressing adenovirus(Ad-GAL) infected group.2) The effect of Ad-EAS virus on the growth of gastric cancer cells in vitro and in vivo.Following infection with EGFR antisense RNA-expressing adenovirus(Ad-EAS), the in vitro growth of Ad-EAS infected cells was significantly inhibited relative to control infected cells in 3 gastric cancer cell lines (AGS, KKLS, MKN28) studied here(p(]SY.di-substituted right.[)O.0002). In a nude mouse subcutaneous tumor system, in vivo tumor growth of MKN28 was significantly inhibited after Ad-EAS treatment, and the inhibition on the 48th day was 93% by volume compared to that of untreated controls.3) The effect of Ad-MAS virus on cancer cell motility in gastric cancer cells.After Ad-MAS virus infection, the cell motility of AGS (c-met expressing) and Hs746t (c-met overexpressing) were significantly inhibited in comparison with control groups. The inhibition rate compare to non-infection group were 88% in AGS and 45% in Hs746t, respectively. These motility inhibition was not observed in KKLS cell (c-met non-expressing).