Experimental therapeutics for gastric cancer using adenovirus vectors

使用腺病毒载体进行胃癌的实验治疗

• 批准号: 08671476
• 负责人: YAMADA Yukishige
• 金额: $ 1.66万
• 依托单位: NARA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671476/
• 关键词: Gastric Cancer; Adenovirus Vector; アデノウィルスベクター; アデノウィルス

1) EGER and c-met expression after EGFR and c-met antisense expressing adenovirus in gastric cancer cellsFollowing infection with EGFR or c-met antisense RNA-expressing adenovirus(Ad-EAS or Ad-MAS), the cell surface EGER or c-met protein levels of infected cancer cell were observed by immunocytological method resulting in that markedly reduction of these proteins respectively. These suppression was not observed in non-infection or beta-galactosidase expressing adenovirus(Ad-GAL) infected group.2) The effect of Ad-EAS virus on the growth of gastric cancer cells in vitro and in vivo.Following infection with EGFR antisense RNA-expressing adenovirus(Ad-EAS), the in vitro growth of Ad-EAS infected cells was significantly inhibited relative to control infected cells in 3 gastric cancer cell lines (AGS, KKLS, MKN28) studied here(p(]SY.di-substituted right.[)O.0002). In a nude mouse subcutaneous tumor system, in vivo tumor growth of MKN28 was significantly inhibited after Ad-EAS treatment, and the inhibition on the 48th day was 93% by volume compared to that of untreated controls.3) The effect of Ad-MAS virus on cancer cell motility in gastric cancer cells.After Ad-MAS virus infection, the cell motility of AGS (c-met expressing) and Hs746t (c-met overexpressing) were significantly inhibited in comparison with control groups. The inhibition rate compare to non-infection group were 88% in AGS and 45% in Hs746t, respectively. These motility inhibition was not observed in KKLS cell (c-met non-expressing).

1) EGFR和c-met反义表达腺病毒感染胃癌细胞后，用免疫细胞学方法观察EGFR或c-met反义rna表达腺病毒（Ad-EAS或Ad-MAS）感染后，感染癌细胞细胞表面EGER和c-met蛋白水平分别显著降低。在未感染或表达β -半乳糖苷酶的腺病毒（Ad-GAL）感染组中未观察到这种抑制。2) Ad-EAS病毒对体外和体内胃癌细胞生长的影响。在本研究的3种胃癌细胞系（AGS、KKLS、MKN28）中，用表达EGFR反sense rna的腺病毒（Ad-EAS）感染后，Ad-EAS感染细胞的体外生长明显受到抑制(p（]SY）。di-substituted [O.0002)。在裸鼠皮下肿瘤系统中，Ad-EAS处理后，MKN28在体内的肿瘤生长明显受到抑制，与未处理的对照组相比，第48天的抑制量为93%。3) Ad-MAS病毒对胃癌细胞运动的影响。Ad-MAS病毒感染后，与对照组相比，表达c-met的AGS和过表达c-met的Hs746t的细胞活力明显受到抑制。与未感染组相比，AGS和Hs746t的抑制率分别为88%和45%。这些运动抑制在KKLS细胞中未观察到（c-met不表达）。

项目成果

T.Hirao: "Antisense EGFR Delivered by Adenoviral Vector Blocks Tumor Growth in Human Gastric Cancer" Cancer gene therapy. (in press). (1999)

T.Hirao：“腺病毒载体传递的反义 EGFR 阻断人类胃癌中的肿瘤生长”癌症基因治疗。

T.HIRAO , H.SAWADA, et.al.: "Antisense EGFR delivered by adenoviral vector blocks tumor growth in human gastric cancer." Cancer Gene Therapy. (in press). (1999)

T.HIRAO、H.SAWADA 等人：“腺病毒载体递送的反义 EGFR 可阻断人胃癌中的肿瘤生长。”

H.Sawada: "Efficiency of gene transfer into human gastric carcinoma cells using adenovirus vector" Progress in Gastric Cancer Research. 677-681 (1997)

H.Sawada：“使用腺病毒载体将基因转移到人胃癌细胞中的效率”胃癌研究进展。

H.SAWADA et.al.: "Efficiency of gene transfer into human gastric carcinoma cells using adenovirus vector" Progress in Gastric Cancer Research. 677-681 (1997)

H.SAWADA 等人：“使用腺病毒载体将基因转移到人胃癌细胞中的效率”胃癌研究进展。