消化器癌におけるオーロラ遺伝子異常およびオーロラを標的とした治療法の可能性の検討

胃肠癌中极光基因异常的研究以及针对极光治疗的可能性

• 批准号: 11671266
• 负责人: YAMADA Yukishige
• 金额: $ 2.3万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671266/
• 关键词: aurora2; amplification; overexpresion; gastric carcinoma; オーロラ遺伝子; 消化器癌

Aurora2 is localized on chromosome 20q13, a region amplified in various human carcinomas and cell lines. Aurora2 encodes cell-cycle-regulated serine / threonine kinase, which has essential functions for centrosome maturation and chromosome segregation. In the present study, we examined the amplification and expression of aurora2 in 9 gastric carcinoma cell lines and 88 primary gastric carcinomas in comparison with clinic pathological features. Aurora2 amplification and over expression was observed in 29 % (2 of 7) and 44.4 % (4 of 9) of cell lines, and 12.5 % (3 of 24) and 41 % (36 of 88) of primary carcinomas. There was discordance between gene amplification and transcript over expression in both gastric carcinoma cell lines and primary carcinoma. Aurora2 over xpression showed significant association with differentiated type gastric carcinoma, and it was also detected in early stage gastric cancer as well as in gastric intestinal metaplasia. Hence, aurora2 over expression is possibly involved in human gastric carcinogensis.

Aurora 2位于染色体20 q13上，该区域在各种人类癌症和细胞系中扩增。Aurora 2编码一种细胞周期调控的丝氨酸/苏氨酸激酶，该激酶在中心体成熟和染色体分离中具有重要功能。本研究检测了Aurora 2在9种胃癌细胞系和88例原发性胃癌中的扩增和表达，并与临床病理特征进行了对照。Aurora 2扩增和过表达分别见于29%（2/7）和44.4%（4/9）的细胞系，以及12.5%（3/24）和41%（36/88）的原发癌。胃癌细胞系和原发癌中基因扩增和转录本过表达存在不一致性。Aurora 2在分化型胃癌中表达明显，在早期胃癌和胃肠上皮化生中也有表达。因此，aurora 2的过度表达可能参与了胃癌的发生。