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Investigation of intermediate filaments in astrocytomas

星形细胞瘤中间丝的研究

基本信息

批准号：
07671556
负责人：
KOBAYASHI Shu
金额：
$ 1.47万
依托单位：
Tokyo Metropolitan Institute of Gerontology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671556/
关键词：
astrocytoma intermediate filament GFAP vimentin phosphorylation

项目摘要

The aim of this project was to evaluate the role of phosphorylation-dephosphorylation regulating assembly of intermediate filament proteins in astrocytic tumors, and to clarify the biological characteristics of gliomas. GFAP (glial fibrillary acidic protein) and vimentin are two major intermediate filament proteins of astroglia. Pertinent findings are described as below, 1) We conducted immunohistochemical studies of specimen removed from 20 patients with low-grade astrocytomas (Grade II) and 21 patients with malignant gliomas including 10 anaplastic astrocytomas (Grade III) and 11 glioblastomas. Anti-phosphorylated GFAP antibody (pG2) did not stain the tissues of astrocytoma Grade II nor Rosenthal fibers. Both were strongly GFAP-positive. 2) In 5 among 21 specimens obtained from patients with malignant gliomas including anaplastic astrocytomas and glioblastomas, pG2 positively stained the tumor cells. Two were glioblastomas and 3 anaplastic astrocytomas. While pG2 stained only mitotic cells which gained phosphorylated GFAP in experiments performed elsewhere using U251 monolayr cells, our results showed the stained cells were mainly in interphase. 3) We found no difference between patients with pG2-positive tumors and those with negative tumors in evaluating time-intervals after treatment to recurrence and survival time. 4) We obtained non-specific findings in experiments studying astrocytic tumors using anti-vimentin antibodies. Experiments using anti-phosphorylated vimentin antibodies which are newly developed in our laboratory, adding the experiments of stored frozen specimen, are in progress.

本项目旨在评估磷酸化-去磷酸化在星形细胞肿瘤中调节中间丝蛋白组装的作用，并阐明胶质瘤的生物学特性。胶质原纤维酸性蛋白（glial fibrillary acid protein， GFAP）和波形蛋白（vimentin）是星形胶质细胞中两种主要的中间丝蛋白。相关发现如下：1)我们对20例低级别星形细胞瘤（II级）患者和21例恶性胶质瘤患者的标本进行免疫组化研究，其中间变性星形细胞瘤（III级）10例，胶质母细胞瘤11例。抗磷酸化GFAP抗体（pG2）未染色星形细胞瘤II级组织和Rosenthal纤维。两者均为gap阳性。2)在21例恶性胶质瘤（包括间变性星形细胞瘤和胶质母细胞瘤）患者的标本中，有5例肿瘤细胞pG2阳性染色。2例为胶质母细胞瘤，3例为间变性星形细胞瘤。虽然pG2只在其他地方使用U251单层细胞进行的实验中染色获得磷酸化GFAP的有丝分裂细胞，但我们的结果显示染色的细胞主要处于间期。3) pg2阳性肿瘤患者与pg2阴性肿瘤患者在治疗后评估复发时间间隔和生存时间方面无差异。4)我们使用抗vimentin抗体研究星形细胞肿瘤的实验获得了非特异性发现。本实验室新开发的抗磷酸化vimentin抗体的实验，加上保存的冷冻标本实验，正在进行中。