Cell-Biological Study of Degenerative Structures (Rosenthal Fibers) in Gliomas.

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The aim of this project was to evaluate the degenerative process occuring in the cytoplasms of low-grade gliomas, such as Rosenthal fibers (RFs), and to clarify the biological characteristics of gliomas. Pertinent findings are described as below ; (1) Immunohistochemically we investigated RFs on specimen surgically removed from patients with qliomas (three cerebellar astrocytomas, three optic gliomas, two spinal cord astrocytomas, one spinal ganglioglioma).We utilized sections from the formalin-fixed paraffin-embedded tissues and stained them with anti-GFAP antibody (Ab) and two anti-ubiquitin Abs... anti-PHF (paired helical filament) monoclonal Ab (DF2) which recognizes ubiquitin and anti-ubiquitin polyconal Ab provided by Dr. Haas. RFs were eosinophilic (brightred on H&E), purply-stained with PTAH (metachromasia), black with Heidemhein's iron-hematoxylin, and negative with PAS. The peripheral parts of RFs were intensely stained with anti-GFAP. DF2 showed intense staining on the ring … More around negative central core of most RFs in every specimen. Anti-ubiquitin polyclonal Ab gave the similar results as DF2. From the study on the serial sections, the peripheral halo of RFs was positive with both anti-GFAP and anti-ubiquitin Abs.Both GFAP and ubiquitin were considered to exist in the periphery of RFs and they might play an important role to produce the amorphous core RFs. (2) We recognized granular bodies (Gb) in the cystoplasm of the glioma tissues among several cases described above, which are also considered to be degenerative change. Whole parts of Gbs were dark-blue on PTAH and positively stained with both GFAP and ubiquitin (a different finding from RFs). (3) We also showed the positive staining with GFAP and ubiguitin in the peripheral parts of RFs which appeared on the cyst wall of cerebellar hemangioblastoma in an autopsied case with massive cerebral hemorrhage. Recently, T. Iwaki reported that alphaB crystallin is the major constituent of RFs from the brain of Alexander disease. In progress is the investigation to evaluate what kind of role alphaB crystallin is playing in the glia and glioma cells by making a specific anti-alphaB crystallin antibody. Less