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Cell-Biological Study of Degenerative Structures (Rosenthal Fibers) in Gliomas.

神经胶质瘤退行性结构（罗森塔尔纤维）的细胞生物学研究。

基本信息

批准号：
01570825
负责人：
KOBAYASHI Shu
金额：
$ 1.34万
依托单位：
Tokyo Metropolitan Institute of Gerontology
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570825/
关键词：
Rosenthal fiber Glioma Degeneration Ubiquitin GFAP Crystallin

项目摘要

The aim of this project was to evaluate the degenerative process occuring in the cytoplasms of low-grade gliomas, such as Rosenthal fibers (RFs), and to clarify the biological characteristics of gliomas. Pertinent findings are described as below ; (1) Immunohistochemically we investigated RFs on specimen surgically removed from patients with qliomas (three cerebellar astrocytomas, three optic gliomas, two spinal cord astrocytomas, one spinal ganglioglioma).We utilized sections from the formalin-fixed paraffin-embedded tissues and stained them with anti-GFAP antibody (Ab) and two anti-ubiquitin Abs... anti-PHF (paired helical filament) monoclonal Ab (DF2) which recognizes ubiquitin and anti-ubiquitin polyconal Ab provided by Dr. Haas. RFs were eosinophilic (brightred on H&E), purply-stained with PTAH (metachromasia), black with Heidemhein's iron-hematoxylin, and negative with PAS. The peripheral parts of RFs were intensely stained with anti-GFAP. DF2 showed intense staining on the ring … More around negative central core of most RFs in every specimen. Anti-ubiquitin polyclonal Ab gave the similar results as DF2. From the study on the serial sections, the peripheral halo of RFs was positive with both anti-GFAP and anti-ubiquitin Abs.Both GFAP and ubiquitin were considered to exist in the periphery of RFs and they might play an important role to produce the amorphous core RFs. (2) We recognized granular bodies (Gb) in the cystoplasm of the glioma tissues among several cases described above, which are also considered to be degenerative change. Whole parts of Gbs were dark-blue on PTAH and positively stained with both GFAP and ubiquitin (a different finding from RFs). (3) We also showed the positive staining with GFAP and ubiguitin in the peripheral parts of RFs which appeared on the cyst wall of cerebellar hemangioblastoma in an autopsied case with massive cerebral hemorrhage. Recently, T. Iwaki reported that alphaB crystallin is the major constituent of RFs from the brain of Alexander disease. In progress is the investigation to evaluate what kind of role alphaB crystallin is playing in the glia and glioma cells by making a specific anti-alphaB crystallin antibody. Less

本研究的目的是评价低级别胶质瘤细胞中发生的退行性变化，如罗森塔尔纤维（RF），并阐明胶质瘤的生物学特性。本研究的主要结果如下：（1）应用免疫组化方法，对3例小脑星形细胞瘤、3例视神经胶质瘤、2例脊髓星形细胞瘤、1例脊神经节胶质瘤的手术切除标本进行了RFs的研究。采用福尔马林固定石蜡包埋切片，用抗GFAP抗体和两种抗泛素抗体染色，结果表明：（1）RFs在小脑星形细胞瘤、视神经胶质瘤、脊髓星形细胞瘤、脊神经节胶质瘤中的表达与胶质细胞的分化程度有关。（2）RFs的表达与胶质细胞瘤的分化程度有关。（3）RFs的表达与胶质细胞瘤的分化程度有关。（3）RFs的表达与胶质细胞瘤的分化程度有关。（4）RFs的表达与胶质细胞瘤的分化程度有关。（5）RFs的表达与胶质细胞瘤的分化程度有关。（5）RFs的表达与胶质细胞瘤的分化程度有关。（6）RFs的表达与胶质细胞瘤的分化程度有关。识别泛素的抗PHF（成对螺旋丝）单克隆抗体（DF 2）和由哈斯博士提供的抗泛素多锥形抗体。RF为嗜酸性（H&E上为亮红色），PTAH（异染性）呈紫红色，Heidemhein铁苏木素呈黑色，PAS呈阴性。GFAP在RFs周围呈强阳性反应。DF2在环上显示强染色 ...更多信息在每个样本中，大多数RF的负中心核周围。抗泛素多克隆抗体得到与DF2相似的结果。从连续切片的研究来看，抗GFAP和抗泛素抗体均呈阳性反应，提示GFAP和泛素均存在于RFs的外周，它们可能在无定形核心RFs的形成中起重要作用。(2)我们在上述几个病例中发现胶质瘤组织的胞浆中存在颗粒体（Gb），这也被认为是退行性改变。Gbs的整个部分在PTAH上呈深蓝色，GFAP和泛素染色阳性（与RF不同的发现）。(3)在1例大出血的小脑血管母细胞瘤的尸检标本中，我们还观察到GFAP和泛素在其周围部分的阳性染色。最近，T. Iwaki报道，α B晶体蛋白是来自亚历山大病脑的RF的主要成分。正在进行的研究是通过制备特异性抗α B晶体蛋白抗体来评估α B晶体蛋白在神经胶质细胞和神经胶质瘤细胞中发挥什么样的作用。少