Effect of electrical stimulation of cerebral cortex on the induction of c-fos in intractable pain model animal

电刺激大脑皮层对顽固性疼痛模型动物c-fos诱导的影响

• 批准号: 07671552
• 负责人: KURODA Ryotaro
• 金额: $ 0.32万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671552/
• 关键词: Pain; c-fos; electrical stimulation of motor-sensory cortex; trigeminal nerve; formalin-induced pain; ホルマリン発痛; 中枢神経

Objective :Expression of the proto-oncogene c-fos has been demonstrated in CNS neurons to be a "third messenger" in long term response to painful stimuli and to be useful as a metabolic marker of brain activity. On the other hand, electrical stimulation of the motor cortex was reported to alleviate the intractable pain. Mechanism of this cortical stimulation therapy has not well recognized. We, therefore, examined the stimulation effect in the sensory-motor cortex on expression of c-fos after formalin injection into the trigeminal periphery of the rat.Methods :After induction of urethane anesthesia, either 0.1ml of 5% formalin or physiological saline was injected into the right mandibular region of the trigeminal nerve in each five rats. Electrical stimulation (1mA,10Hz) in the left sensory-motor cortex for the period of one hour was applied two hours after the facial injection of formalin or saline in another five rats each. All these rats were perfused and fixed three hours after the … More facial injection and processed immuno-histochemicaly to visualize c-fos expression in CNS.The number of Fos positive neurons in the nucleus caudalis of spinal trigeminal nucleus was statistically analyzed in each group using ANOVA methods.Results and Conclusion :Formalin injection induced c-fos expression strongly in I/II layr of the trigeminal caudalis nucleus of the same side, compared with the saline injection group. C-fos was also expressed in the brain stem, hypothalamus and cerebral cortex in both groups. The electrical stimulation induced c-fos expression in the cerebral cortex of the stimulated side, compared with the contralateral side. ANOVA demonstrated that electrical stimulation reduced significantly the expression of c-fos in the I/II layr of the caudalis nucleus, while increased the expression in the III/IV layr of the formalin injected rats, compared with other groups.This fact suggests that cortical stimulation suppresses the activity of small neurons in the I/II layr and enhances large neurons in the III/IV layr of the trigeminal caudalis nucleus, which is reminiscent of the gate theory. Electrical stimulation also activated the cortical neurons not only in the sensory-motor area, but in the neighboring areas. The full significance of this study and mechanism of the motor cortex stimulation therapy are still to be studied. Less

目的：原癌基因c-fos在中枢神经系统（CNS）神经元中的表达已被证实是疼痛刺激长期反应的“第三信使”，并可作为脑活动的代谢标志物。另一方面，据报道，电刺激运动皮层可缓解顽固性疼痛。这种皮层刺激疗法的机制还没有得到很好的认识。因此，我们研究了在感觉运动皮层的刺激效果后，福尔马林注射到三叉神经周围的大鼠c-fos的表达。方法：在诱导聚氨酯麻醉后，无论是0.1ml的5%福尔马林或生理盐水注射到右下颌区的三叉神经在每5只大鼠。在另外5只大鼠的面部注射福尔马林或生理盐水后2小时，在左侧感觉-运动皮层中施加电刺激（1 mA，10 Hz）持续1小时。所有这些大鼠在注射后3小时进行灌注和固定。 ...更多信息 结果与结论：与生理盐水组相比，福尔马林注射组在同侧三叉神经脊束核尾侧核的I/II层有较强的c-fos表达。两组大鼠脑干、下丘脑和大脑皮质均有C-fos表达。与对侧相比，电刺激诱导受刺激侧大脑皮质c-fos表达。方差分析显示，电刺激可显著降低福尔马林注射组大鼠三叉神经尾侧核I/II层c-fos的表达，而增加III/IV层c-fos的表达，这一事实表明，皮层刺激抑制三叉神经尾侧核I/II层小神经元的活动，增强III/IV层大神经元的活动，这让人想起了星门理论电刺激不仅激活了感觉运动区的皮层神经元，还激活了邻近区域的皮层神经元。这项研究的意义和运动皮层刺激疗法的机制仍有待研究。少

项目成果

R.Kuroda et al: "Frontal cingulotomy reconsidered from a WGA-HRP and c-fos study in cat" Acta Neurochir. S64. 69-73 (1995)

R.Kuroda 等人：“根据猫的 WGA-HRP 和 c-fos 研究重新考虑额叶扣带回切开术”Acta Neurochir。

R.Kuroda et al: "c-fos expression after formalin injection into the face in the cat" Stereotact Func Neurosurgery. 65. 152-156 (1995)

R.Kuroda 等人：“猫面部注射福尔马林后 c-fos 的表达”Stereotact Func Neurosurgery。

R. Kuroda: "c-fos expression after pain stimulation (formalin injection) of the face in cats" Stereotactic and Functional Neurosurgery. 64/2. 95- (1995)

R. Kuroda：“猫面部疼痛刺激（福尔马林注射）后 c-fos 的表达”立体定向和功能神经外科。