Specific expression of molecules in the tissues of cholesteatoma otitis media -3'prime cDNA subtraction method analysis-

胆脂瘤中耳炎组织中分子的特异性表达-3prime cDNA消减法分析-

• 批准号: 07671854
• 负责人: DOI Katsumi
• 金额: $ 1.66万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671854/
• 关键词: cholesteatoma; 3'prime cDNA; differential display; HGF; C-MET; Tpr; 3′プライム方向cDNA; C-MET; IL-6; MMP-9

1.Messenger ENAs (mRNAs) in tissues of cholesteatoma otitis media (Chole), chronic purulent otitis media (OMC), and skin of external ear canal (Skin) were extracted and reverse-transcribed into the cDNAs. These cDNAs were compared and analyzed by using the 3' prime cDNA library subtraction method and the differential display method.2.The 3' prime cDNA library subtraction indicated that the expressions of mRNAs of hepatocyte growth factor (HGF)and c-met, the specific HGF receptor, were up-regulated in the Chole-cDNA library, and that the copy numbers for these cDNAs were extremely higher in this library than those in the OMC and skin-cDNAs libraries.3.The differential display also demonstrated that the mRNA expression of Tpr (translocated promoter region) gene, a component of the nuclear pore complex, was also up-regulated in the Chole-cDNAs.4.It has been suggested that HGF/c-met system should play an important role in poliferation of several epithelial and non-epithelial cells including keratinocyte. Although potential functions of Tpr gene have not been determined, Tpr protein has been suggested to be a phosphoprotein and have a transforming activity.The present study suggests that both HGF/c-met system and Tpr gene could be involved in proliferation of keratinocytes and development of granulation tissues observed in cholesteatoma otitis media.

1.提取胆脂瘤性中耳炎（Chole）、慢性化脓性中耳炎（OMC）和外耳道皮肤（skin）组织中的信使ENAs （mrna）并将其反向转录为cdna。采用3' prime cDNA文库减法和差异显示法对这些cDNA进行比较分析。3' ' prime cDNA文库减法结果表明，在Chole-cDNA文库中，肝细胞生长因子（HGF）和特异性HGF受体c-met的mrna表达上调，且其拷贝数显著高于OMC和皮肤cDNA文库。差异显示还表明，核孔复合体的组成部分Tpr（易位启动子区）基因的mRNA表达也在chole - cdna中上调。HGF/c-met系统在包括角质形成细胞在内的多种上皮和非上皮细胞的增殖中发挥重要作用。虽然Tpr基因的潜在功能尚未确定，但Tpr蛋白被认为是一种磷酸化蛋白，具有转化活性。本研究提示HGF/c-met系统和Tpr基因均参与了胆脂瘤性中耳炎中角质形成细胞的增殖和肉芽组织的发育。