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DNA microarray analysis on frequency tuning and tonotopical organization in the mouse cochlea

DNA 微阵列分析小鼠耳蜗频率调谐和音调组织

基本信息

批准号：
15591811
负责人：
DOI Katsumi
金额：
$ 2.18万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591811/
关键词：
Cochlea DNA microarray analysis apical turn basal turn real time PCR frequency tunig 周波数選択制回転別 TOTAL RNA

项目摘要

To understand the mechanism of frequency tuning and tonotopical organization in the mouse cochlea, we characterized mRNA expression in the apical and basal parts of the mouse cochlea with a DNA microarray analysis.DNA microarray analysis revealed that the mRNA expression of 184 out of 19801 genes on the DNA chip was more than three times up-regulated in the apical part of the mouse cochlea and that the mRNA expression of 181 out of 19801 genes on the DNA chip was more than three times up-regulated in the basal part. Further study by using a real-time PCR technique confirmed that the expressions of Slc4al (Cl-HC03 exchanger) was up-regulated by 3.12 times in the apical part and that the expressions of six genes including Gabt1 (GABA receptor β1), Nstr2 (Neurotensin receptor), and Tac1 (Substance P) were up-regulated more than three times in the basal part.DNA microarray analysis focusing on the efferent neurotransmission via acetylcholine receptors (AChR) and GABA receptors (GABAR) demonstrated that the mRNA expression of AChR subunits was almost comparable in the apical and basal parts of the mouse cochlea while the mRNA expression of GABAR subunits was not always even in both parts. The mRNA expressions of subunits γ3,θ,δ, and ρ were almost equal in both parts. However, the expressions of β1,β2,γ1, and γ2 were more than two times up-regulated in the basal part of the mouse cochlea.These results indicate that the location/place-specific mRNA expression in hair cells and spiral ganglion cells in the mouse cochlea might determine the frequency tuning and tonotopical organization.

为了了解小鼠耳蜗频率调谐和音调组织的机制，我们用DNA微阵列分析了小鼠耳蜗顶端和基底部的mRNA表达。DNA微阵列分析显示，在DNA芯片上的19801个基因中，有184个基因的mRNA表达比对照组高出3倍以上。在小鼠耳蜗的顶端部分中调节，并且在DNA芯片上的19801个基因中的181个基因的mRNA表达在基底部分中上调超过三倍。通过使用实时PCR技术的进一步研究证实，Slc 4al的表达在细胞中是稳定的。C1-HC 03交换器基因在根尖部的表达上调了3.12倍，Gabt 1等6个基因的表达在根尖部的表达上调了3.12倍，（GABA受体β1），Nstr 2（神经降压素受体），Tac1（P物质）上升-DNA微阵列技术研究乙酰胆碱受体（AChR）介导的传出神经传递和γ-氨基丁酸受体（GABAR）的表达结果表明，AChR亚基在耳蜗顶、底部的表达基本一致，而GABAR亚基的表达并不一致。γ3、θ、δ和ρ亚基的mRNA表达在两个部位几乎相等。而在小鼠耳蜗基底部，β1、β2、γ1和γ2的表达上调了2倍以上，提示小鼠耳蜗毛细胞和螺旋神经节细胞的mRNA表达可能决定了频率调谐和音调结构。