Effects or hypergravity on genes expressed in the rat inner car

超重力对大鼠内车基因表达的影响

• 批准号: 13671778
• 负责人: DOI Katsumi
• 金额: $ 2.3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671778/
• 关键词: hypergravity; gent expression; glutamate receptor; osteopontine; cochlea, vestibule; グルタミン酸受容体; 重力負荷; オステオポンチン; mRNA; AQP1; AQP3

Recovery from balance disorders aster spaceflight is supposed.to reflect the readaptation to normal gravitational environment (1G). from microgravity, In the present study, to elucidate a role of otoconia in adaptation to altered gravity, we examined the effects of hypergravity (2G) on nor polo and synthesis of otoconia in young adult rats, Neither otoconial morphology nor otoconial synthesis was affected by up to 1 week exposure to hypergravity, These results suggest that changes in neurotransmission at the synapses or flit peripheral and/or contral vestibular system rather than the Changes in otoconial morphology and synthesis may be involved in adaptive mechanismsWe also assessed the changes in mRNA expression of glutamate receptors in the vestibular system of rats exposed to hypergravily for 2 h to 1 week using real-time quantitative RT-PCR, Tho expression of GluR2 and NRI in khe uvula/nodulus and NR1 in the medial vestibuiar nucleus increased after 2 h of hypergravity, The expresion of GluR2 in vestibular ganglion cells decreased after 1 week of hypergravity ; The results suggested that the animals adapted to the hypergravity by enhancing the crrebellar inhibition of the vestibular nucleus via an activation of the NR1 and GluR2 on tha Purkinje cells in uvula/nodules especially of the early phase following hypergravity, while in the later phase by reducing the neurotransmission between the vestibular hair cells and the primary vestibular neurons via down-regulation of the postsynaptic GluR2 in the vestibular periphery

从平衡紊乱中恢复的supposed.to反映了对正常重力环境的重新适应（1G）。为了阐明耳石在适应改变的重力中的作用，我们研究了超重力（2G）对年轻成年大鼠耳石形态和合成的影响。超重力暴露长达1周，耳石形态和耳石合成均未受到影响，这些结果表明，在突触或flit外周和/或神经传递的变化，耳石形态和合成的改变可能参与了适应机制。我们还观察了暴露于谷氨酸的大鼠前庭系统中谷氨酸受体mRNA表达的变化。实时荧光定量RT-PCR检测结果显示，失重2 h后，大鼠悬雍垂/小结中GluR 2、NRI和前庭内侧核中NR 1的表达增加，而1周后，前庭神经节细胞中GluR 2的表达减少;结果提示，动物通过激活前庭核的NR 1和GluR 2，增强前庭核的小脑抑制，从而适应超重。在超重后早期，通过下调前庭外周突触后GluR 2，减少前庭毛细胞和初级前庭神经元之间的神经传递，

项目成果

Uno Y., Horii A., Uno A., Fuse Y., Fukushima M., Doi K., Kubo T.: "Quantitative changes in mRNA expression of glutamate receptors in the rat peripheral and central vestibular systems following hypergravity."J.Neurochem.. 81. 1308-1317 (2002)

Uno Y.、Horii A.、Uno A.、Fuse Y.、Fukushima M.、Doi K.、Kubo T.：“超重力后大鼠外周和中央前庭系统中谷氨酸受体 mRNA 表达的定量变化。”J

Uno Y., Horii A., Uno A., Fuse Y., Fukushima M., Doi K., Kubo T.: "Quantitative changes in mRNA expression of glutamate receptors in the rat peripheral and central vestibularsystems following hypergravity"J.Neurochem. 81. 1308-1317 (2002)

Uno Y.、Horii A.、Uno A.、Fuse Y.、Fukushima M.、Doi K.、Kubo T.：“超重力后大鼠外周和中枢前庭系统中谷氨酸受体 mRNA 表达的定量变化”J.Neurochem

Uno Y., Horii A., Umemoto M., Hasegawa T., Doi K., Uno A., Takemura T., Kubo T.: "Effects of hypergravity on morphology and osteopontin expression in the rat otolith organs."J.Vest.Res.. 10. 283-289 (2000)

Uno Y.、Horii A.、Umemoto M.、Hasekawa T.、Doi K.、Uno A.、Takemura T.、Kubo T.：“超重力对大鼠耳石器官形态和骨桥蛋白表达的影响。”

Uno Y., Horii A., Umemoto M., Hasegawa T., Doi K., Uno A., Takemura T., Kubo T.: "Effects of hypergravity on morphology and osteopontin expression in the rat otollth organs"J.Vest.Res.. 10. 283-289 (2000)

Uno Y.、Horii A.、Umemoto M.、Hasekawa T.、Doi K.、Uno A.、Takemura T.、Kubo T.：“超重力对大鼠耳器官形态和骨桥蛋白表达的影响”J.Vest