Effects or hypergravity on genes expressed in the rat inner car

超重力对大鼠内车基因表达的影响

• 批准号: 13671778
• 负责人: DOI Katsumi
• 金额: $ 2.3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671778/
• 关键词: hypergravity; gent expression; glutamate receptor; osteopontine; cochlea, vestibule; グルタミン酸受容体; 重力負荷; オステオポンチン; mRNA; AQP1; AQP3

Recovery from balance disorders aster spaceflight is supposed.to reflect the readaptation to normal gravitational environment (1G). from microgravity, In the present study, to elucidate a role of otoconia in adaptation to altered gravity, we examined the effects of hypergravity (2G) on nor polo and synthesis of otoconia in young adult rats, Neither otoconial morphology nor otoconial synthesis was affected by up to 1 week exposure to hypergravity, These results suggest that changes in neurotransmission at the synapses or flit peripheral and/or contral vestibular system rather than the Changes in otoconial morphology and synthesis may be involved in adaptive mechanismsWe also assessed the changes in mRNA expression of glutamate receptors in the vestibular system of rats exposed to hypergravily for 2 h to 1 week using real-time quantitative RT-PCR, Tho expression of GluR2 and NRI in khe uvula/nodulus and NR1 in the medial vestibuiar nucleus increased after 2 h of hypergravity, The expresion of GluR2 in vestibular ganglion cells decreased after 1 week of hypergravity ; The results suggested that the animals adapted to the hypergravity by enhancing the crrebellar inhibition of the vestibular nucleus via an activation of the NR1 and GluR2 on tha Purkinje cells in uvula/nodules especially of the early phase following hypergravity, while in the later phase by reducing the neurotransmission between the vestibular hair cells and the primary vestibular neurons via down-regulation of the postsynaptic GluR2 in the vestibular periphery

从平衡失调中恢复是在太空飞行中被认为的。以反映对正常重力环境（1G）的重新适应。在本研究中，为了阐明耳孔在适应重力变化中的作用，我们研究了超重力（2G）对年轻成年大鼠耳孔形态和耳孔合成的影响。暴露在超重力环境中长达1周，耳孔形态和耳孔合成都没有受到影响。这些结果表明，突触或外周和/或控制性前庭系统的神经传递变化可能参与适应机制，而不是耳锥形态和合成的变化。我们还利用实时定量RT-PCR评估了暴露于超重2小时至1周的大鼠前庭系统谷氨酸受体mRNA表达的变化。超重2 h后，小舌/结节GluR2、NRI及前庭内侧核NR1表达升高，超重1周后，前庭神经节细胞GluR2表达降低；结果表明，动物适应超重力后，小脑对前庭核的抑制作用通过激活小叶/结节浦肯野细胞上的NR1和GluR2而增强，特别是在超重力后的早期，而在后期，前庭毛细胞和初级前庭神经元之间的神经传递通过下调前庭外周突触后GluR2而减少

项目成果

Uno Y., Horii A., Uno A., Fuse Y., Fukushima M., Doi K., Kubo T.: "Quantitative changes in mRNA expression of glutamate receptors in the rat peripheral and central vestibular systems following hypergravity."J.Neurochem.. 81. 1308-1317 (2002)

Uno Y.、Horii A.、Uno A.、Fuse Y.、Fukushima M.、Doi K.、Kubo T.：“超重力后大鼠外周和中央前庭系统中谷氨酸受体 mRNA 表达的定量变化。”J

Uno Y., Horii A., Uno A., Fuse Y., Fukushima M., Doi K., Kubo T.: "Quantitative changes in mRNA expression of glutamate receptors in the rat peripheral and central vestibularsystems following hypergravity"J.Neurochem. 81. 1308-1317 (2002)

Uno Y.、Horii A.、Uno A.、Fuse Y.、Fukushima M.、Doi K.、Kubo T.：“超重力后大鼠外周和中枢前庭系统中谷氨酸受体 mRNA 表达的定量变化”J.Neurochem

Uno Y., Horii A., Umemoto M., Hasegawa T., Doi K., Uno A., Takemura T., Kubo T.: "Effects of hypergravity on morphology and osteopontin expression in the rat otolith organs."J.Vest.Res.. 10. 283-289 (2000)

Uno Y.、Horii A.、Umemoto M.、Hasekawa T.、Doi K.、Uno A.、Takemura T.、Kubo T.：“超重力对大鼠耳石器官形态和骨桥蛋白表达的影响。”

Uno Y., Horii A., Umemoto M., Hasegawa T., Doi K., Uno A., Takemura T., Kubo T.: "Effects of hypergravity on morphology and osteopontin expression in the rat otollth organs"J.Vest.Res.. 10. 283-289 (2000)

Uno Y.、Horii A.、Umemoto M.、Hasekawa T.、Doi K.、Uno A.、Takemura T.、Kubo T.：“超重力对大鼠耳器官形态和骨桥蛋白表达的影响”J.Vest