THE EFFECT OF OVERPRODUCTION OF ENDOLYMPH ON THE INNER EAR FUNCTION

内淋巴分泌过多对内耳功能的影响

• 批准号: 07671863
• 负责人: TAKEDA Taizo
• 金额: $ 1.47万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671863/
• 关键词: Overproduction of endolymph; Endolymphatic hydrops; Endocochlear potential; Action potential of the cochlear nerve; TEOA; DPOAE; K^+ activity; 前庭神経自発活動電位; 人工内リンパ液; Negative EP; DP-OAE

In order to study the effect of an overproduction of the endolymph on the inner ear function, artificial endolymph was injected into the scala media in animals with the experimentally-induced endolymphatic hydrops, and the auditory and vestibular functions were examined. The results were as follows : 1) Endolymphatic hydrops was induced by electro-cauterization of the endolymphatic sac. Moderate or severe hydrops developed 2 months after the cauterization. In these animals, the positive and negative endocochlear potentials had already decreased. Artificial endolymph injection into the cochlear duct produced no significant alteration in these potentials, although the negative endolymphatic potential decreased in normal animals. 2) The compound action potentials and cochlear microphonics also decreased in animals with experimentally induced hydrops. These potentials showed no change before and after the artificial endolymph injection. The delayd CM could not be detected in the present se … More ries of experiments. 3) Transient evoked otoacoustic emission (TEOA) was detected 2 weeks after the cauterization of the sac, but disappeared after 2 months. Thus, the effect of artificial endolymph injection to TEOAE was not investigated. Distortion product otoacoustic emission (DPOAE) could be recorded, but we could not examine the influence of artificial endolymph injection on DPOAE because of its instability. 4) Artificial endolymph injection into the cochlear duct produced no alteration in spontaneous discharge of the vestibular nerve either in normal animals or in animals with experimentally induced hydrops. 5) artificial endolymph injection into the cochlear duct produced no alteration in K^+ activity in the scala tympani either in normal animals or in animals with experimentally induced hydrops.In the previous studies, cochlear and vestibular dysfunction seemed to be attributed to high concentration of K ^+ in the scala media due to the leakage of endolymph via extended Reissner's membrane. However, the present results did not favor this hypothesis. Less

为了研究内淋巴过度分泌对内耳功能的影响，将人工内淋巴注入实验性内淋巴积水动物的中阶，并检查听觉和前庭功能。结果表明：（1）电灼内淋巴囊可诱发内淋巴积水。烧灼后2个月出现中度或重度水肿。在这些动物中，正和负耳蜗内电位已经降低。人工内淋巴注入到耳蜗导管产生的这些电位没有显着的改变，虽然在正常动物的负内淋巴电位下降。2)复合动作电位和耳蜗微音电位在实验诱导的水肿动物中也降低。这些电位在人工内淋巴注射前后无明显变化。延迟CM在目前的条件下无法检测到 ...更多信息 一系列的实验。3)烧灼囊后2周检测到瞬态诱发耳声发射（TEOA），但2个月后消失。因此，未研究人工内淋巴注射对TEOAE的影响。畸变产物耳声发射（DPOAE）可被记录，但由于其不稳定性，我们无法检测人工内淋巴注射对DPOAE的影响。4)人工内淋巴注入耳蜗导管中，无论是在正常动物还是在实验诱导水肿的动物中，前庭神经的自发放电都没有改变。5)在以往的研究中，耳蜗和前庭功能障碍似乎是由于内淋巴通过扩展的Reissner膜渗漏而导致中阶K ^+浓度过高所致。然而，目前的结果并不支持这一假设。少

项目成果

Kakigi A,Takeda T: "Effect of artificial endolymph injection into the cochlear duct on the endocochlear potential." Hearing Research. 116. 113-118 (1998)

Kakigi A，Takeda T：“人工内淋巴注射到耳蜗管对耳蜗内电位的影响。”

Kakigi A: "Effect of artificial endolymph injection into the cochlear duct on the endocochlear potential" Hearing Research. 116. 113-118 (1998)

Kakigi A：“人工内淋巴注入耳蜗管对耳蜗内电位的影响”听力研究。

Taizo Takeda 他: "Effect of artificial endolymph injection into the cochlear duct on the endolcochlear potential" Hearing Res. (発表予定). (1997)

Taizo Takeda 等人：“人工内淋巴注射到耳蜗管对耳蜗电位的影响”（即将发表）。