The role of water channel of aquaporins and water pump of Na-K-2Cl co-transporter on the water homeostasis of the inner ear.

水通道蛋白的水通道和Na-K-2Cl协同转运蛋白的水泵对内耳水稳态的作用。

• 批准号: 20591987
• 负责人: TAKEDA Taizo
• 金额: $ 3.08万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591987/
• 关键词: aquaporin; イオン共輸送体; 血管条; 内リンパ嚢; vasopressin; 水チャネル; NKCC1; NKCC2; stria vascularis; endolymphatic sac; bumetanide; 内耳水代謝

The expressions of many subtypes of aquaporins (AQP) including AQP-2, vasopressin type 2 receptor, and NKCC1 were molecular biologically and immunohistochemically evaluated. Since the application of vasopressin, bumetanide, and OPC-31260 exerted the morphological influence on the intrastrial space, AQP2-vasopressin mediated water transport and water flow through the Na-K-Cl co-transporter might work actively in the inner ear fluid homeostasis.

采用分子生物学和免疫组织化学方法对AQP-2、抗利尿激素2型受体、NKCC1等AQP亚型的表达进行评价。由于加压素、布美他尼和OPC-31260的应用对内耳腔产生形态学影响，因此aqp2 -加压素介导的水转运和Na-K-Cl共转运体的水流可能在内耳流体稳态中起积极作用。

项目成果

Expression and immunolocalization of aquaporin-6 (Aqp6) in the rat inner ear

• DOI: 10.1080/00016480701765691
• 发表时间: 2008-01-01
• 期刊: ACTA OTO-LARYNGOLOGICA
• 影响因子: 1.4
• 作者: Taguchi, Daizo;Takeda, Taizo;Kitano, Hiroya
• 通讯作者: Kitano, Hiroya

Expression of aquaporins and vasopressin type 2 receptor in the stria vascularis of the cochlea

• DOI: 10.1016/j.heares.2009.09.011
• 发表时间: 2010-02
• 期刊: Hearing Research
• 影响因子: 2.8
• 作者: R. Nishioka;T. Takeda;A. Kakigi;Teruhiko Okada;Shinji Takebayashi;Daizo Taguchi;M. Nishimura;Masamitsu Hyodo

Hormonal Aspects of Ménière’s Disease on the Basis of Clinical and Experimental Studies

• DOI: 10.1159/000265113
• 发表时间: 2010-02
• 期刊: ORL
• 作者: T. Takeda;S. Takeda;A. Kakigi;T. Okada;R. Nishioka;Daizo Taguchi;M. Nishimura;H. Nakatani

Expression of aquaporins, vasopressin type 2 receptor, and Na+-K+-Cl– cotransporters in the rat endolymphatic sac

• DOI: 10.1080/00016480802441754
• 发表时间: 2009-01
• 期刊: Acta Oto-Laryngologica
• 影响因子: 1.4
• 作者: M. Nishimura;A. Kakigi;T. Takeda;S. Takeda;K. Doi

Expression of aquaporin1, 2, 3, and 4, NKCC1, and NKCC2 in the human endolymphatic sac

人内淋巴囊中水通道蛋白 1、2、3 和 4、NKCC1 和 NKCC2 的表达

• 发表时间: 2008
• 作者: Kakigi A;Nishimura M;Takeda T;Taguchi D;Nishioka R
• 通讯作者: Nishioka R