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The role of vasopressin to the formation of endolymphatic hydrops

加压素在内淋巴积液形成中的作用

基本信息

批准号：
11671685
负责人：
TAKEDA Taizo
金额：
$ 2.5万
依托单位：
KOCHI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671685/
关键词：
Meniere's disease Endolymphatic hydrops Vasopressin aquaporins 水チャネルアクアポリン

项目摘要

Recently, many lines of evidence have supported the possibilities that vasopressin (VP) has important role in the formation of endolymphatic hydrops in Meniere's disease. The aim of this study is to investigate the role of VP about endolymphatic hydrops. In this study, we examined two experiments. First, we examined whether or not the chronic administration of VP induce endolymphatic hydrops. Second, we investigated the expression pattern of vasopressin receptor (V2 receptor) and V2 specific water channel (aquaporin 2, AQP2).(1) VP was administered to the guinea pigs at the rats of 0.2U/kg/min, 0.4U/kg/min, 1U/kg/min fo 7days. The degree of hydrops was quatitatively assessed by increase ratio of the scala media area in the mid-modiolar sections of the cochlea. Scala media area of 0.4 and 1U/kg/min group was significantly increased compared with control group. We confirmed the high level of VP induce endolymphatic hydrops in the guinea pig.(2) We investigated the expression of V2 receptor and AQP2 mRNA via RT-PCR.The rat cochlea and endolymphatic sac were used. RT-PCR was done by specific primers for V2 receptor and AQP2. PCR products were electrophorased by agarose gel and sequenced. AQP2 and V2 receptor were both expressed in the rat cochlea and endolymphatic sac. These results indicate that vasopressin has important role for water regulation in the inner ear.In conclusion, all data from these experiments support that VP is closely linked to endolymphatic hydrops in Meniere's disease

最近，许多证据支持血管加压素(VP)在梅尼埃病内淋巴积液的形成中起重要作用的可能性。本研究的目的是探讨VP在内淋巴积水中的作用。在这项研究中，我们考察了两个实验。首先，我们检查了长期服用VP是否会引起内淋巴积水。(1)分别以0.2U/kg/min、0.4U/kg/min、1U/kg/min的剂量给豚鼠注射VP，连续7d。以耳蜗骨中部中段的中阶面积增加率来定量评价积水的程度。0.4U/kg/min和1U/kg/min组的中阶面积较对照组显著增加。通过RT-PCR检测V2受体和AQP2mRNA的表达，以大鼠耳蜗囊和内淋巴囊为模型。用V2受体和AQP2特异性引物进行RT-PCR。聚合酶链式反应产物经琼脂糖凝胶电泳后测序。AQP2和V2受体在大鼠耳蜗囊和内淋巴囊均有表达。这些结果表明，加压素在内耳的水分调节中起着重要的作用。综上所述，这些实验数据都支持在梅尼埃病中VP与内淋巴积水密切相关