HLA-DNA ANALYSIS IN CORNEAL TRANSPLANTATION

角膜移植中的 HLA-DNA 分析

• 批准号: 07671936
• 负责人: SHIMAZAKI Jun
• 金额: $ 1.54万
• 依托单位: TOKYO DENTAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671936/
• 关键词: CORNEAL TRANSPLANTATION; HLA CLASS II ANTIGENS; PCR; REIECTION; HLAクラスII抗原; 拒絶反応

ResultsWe have analyzed 81 donor-recipient pairs of corneas from patients undergoing corneal transplantation. Fifty-one of 81 cases had high risk for immunological rejection, and 18 (35.3%) of them developed the rejection postoperatively. Compatibilities of neither HLA-DR,or DQ antigens demonstrated significant correlation with the development of rejection. However, HLA-DP antigen compatibility showed significant correlation with the episode (P=0.024). The correlation was more significant in high-risk cases. Positive stainings of HLA-DR,DQ and DP antigens were observed in corneal tissues by immunohistochemistry. No significant correlation was found between HLA-A antigen compatibility and the development of rejection, probably due to insufficient number of samples analyzed.EvaluationWe have obtained positive results on the effect of HLA class II antigen compatibility and developement of rejection in corneal transplantation. Importance of HLA-DR matching has been reported in renal transplantation, however, there were no studies reporting the importance of HLA-DP antigens in clinical organ transplantation.The results may be related to the unique characteristics of corneal transplantation immunology. Whether or not the HLA-DP matching is important in cases receiving local or systemic immuno-suppression should be studied by increasing the number of analysis.

结果我们分析了81对角膜移植患者的供受者对角膜。81例中有51例发生免疫排斥反应，其中18例(35.3%)术后发生排斥反应。HLA-DR或DQ抗原的相容与排斥反应的发生无明显相关性。而HLADP抗原相容与发病有显著相关性(P=0.024)。这种相关性在高危病例中更为显著。免疫组织化学法检测角膜组织中的HLADR、DQ和DP抗原表达阳性。人类白细胞抗原-A类抗原相容与排斥反应的发生无明显相关性，可能与分析样本数量不足有关。评价：我们在角膜移植中人类白细胞抗原II类抗原相容与排斥反应发生的影响方面取得了积极的结果。人类白细胞抗原-DR配型在肾移植中的重要性已有报道，但尚未有研究报道人类白细胞抗原-DP抗原在临床器官移植中的重要性，这可能与角膜移植免疫学的独特特性有关。在接受局部或全身免疫抑制的病例中，是否需要通过增加分析次数来研究人类白细胞抗原-DP配型是否重要。

项目成果

島崎潤宏.坪田一男.土田文子.佐藤忠之.萩原政夫.辻公美: "角膜移植におけるクラスII主要適合性抗原(HLA)のDNAタイピング." 日本眼科学会雑誌. 99. 1248-1253 (1995)

Junhiro Shimazaki、Kazuo Tsubota、Ayako Tsuchida、Tadayuki Sato、Masao Hagiwara、Kimi Tsuji：“角膜移植中 II 类主要相容性抗原 (HLA) 的 DNA 分型。” 日本眼科学会杂志 99. 1248-1253 (1995)。 ）

B. Munkhbat,: "HLA Class II DNA Typing Using Ocular Tissue and Its Usefulness in Corneal Transplantation." Transplantation Proceedings.28. 1257-1258 (1996)

B. Munkhbat，：“使用眼组织进行 HLA II 类 DNA 分型及其在角膜移植中的用途。”