Differentiation to keratocyte from oral mucosal non-ephthelial cells

从口腔粘膜非上皮细胞分化为角膜细胞

• 批准号: 23592592
• 负责人: SHIMAZAKI Jun
• 金额: $ 3.24万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592592/
• 关键词: 口腔粘膜; 間葉系幹細胞; 神経堤細胞; 角膜実質; 分化誘導

To establish method of differentiation into keratocyte from oral mucosal non-epithelial cells. Oral mucosal non-epithelial cells were isolated from oral mucosal tissues, cultured with methyl cellulose from single cell, and analyzed ability of multi-differentiation to mesenchymal cells, neural cells, or keratocytes. Oral mucosal non-epithelial cells were estimated the expression of mesenchymal stem cell markers. Oral mucosal non-epithelial cells selected neural crest marker, CD56 and PDGFRa, were differentiated into osteoblasts, adipocytes, chondrocytes, or neural cells. Keratocan, a marker of keratocytes, were detected in oral mucosal non-epithelial cells expressed mesenchymal stem cells markers. These data suggested that oral mucosal non-epithelial cells would be expected to a potential cell source for regeneration of corneal stroma.

建立口腔黏膜非上皮细胞向角膜基质细胞分化的方法。从口腔粘膜组织中分离口腔粘膜非上皮细胞，用甲基纤维素进行单细胞培养，分析其向间充质细胞、神经细胞和角膜基质细胞多向分化的能力。检测口腔黏膜非上皮细胞间充质干细胞标志物的表达。选择神经嵴标记物CD 56和PDGFRa的口腔粘膜非上皮细胞分化为成骨细胞、脂肪细胞、软骨细胞或神经细胞。在口腔粘膜非上皮细胞中检测到间充质干细胞标志物角膜蛋白聚糖（keratocan）。这些数据表明，口腔粘膜非上皮细胞有望成为角膜基质再生的潜在细胞来源。

项目成果

口腔粘膜上皮下組織のSingle cellから増殖した細胞の解析とその応用

口腔粘膜上皮下组织单细胞增殖细胞分析及其应用

• 发表时间: 2013
• 作者: 池田陽子;中野正和;比嘉一成,佐竹良之,島崎潤
• 通讯作者: 比嘉一成,佐竹良之,島崎潤