Evaluation of testicular function in the experimental models of cryptorchidism and its new treatment.

隐睾实验模型中睾丸功能的评价及其新的治疗方法。

• 批准号: 07671953
• 负责人: SHONO Takeshi
• 金额: $ 1.47万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671953/
• 关键词: mechanically-induced cryptorchidism; hormonally-induced cryptorchidism; rat; flutamide; orchiopexy; testicular function; Johnsen score; fertility; 停留精巣; 実験モデル; セルトリ細胞; シメチジン; フタル酸エステル

Histological development of the testis and fertility studies were performed in two different cryptorchid rat models which were hormonally-induced and mechanically induced cryptorchidism. Hormonally-induced cryptorchidism was made by prenatal administration of antiandrogen : flutamide and mechanically induced cryptorchidism was made by extraabdominal fixation of gubernaculum in newborn rats. At the age of 90 days, the paternity rate of sexually matured rats showed 83% (n=12) in mechanically induced unilateral cryptorchidism, in contrats the paternity rate was 0% (n=10) in hormonally induced unilateral cryptorchid rats. Johnsen score also expressed the significantly inhibited histological development of the testis in hormonally induced cryptorchidism compared by mechanically induced cryptorchidism.After orchiopesy, furthermore, the results of paternity rate was significantly inhibited in hormonally induced cryptorchidism compared by mechanically induced cryptorchidism.These results suggested that the causes which induce cryptochidism in fetal period would affect not only the postnatal testicular development but also effect the orchiopexy of these rats.

在激素诱导和机械诱导两种不同的隐睾大鼠模型中进行了睾丸组织学发育和生育研究。新生大鼠通过产前给药抗雄激素氟他胺产生激素性隐睾，通过腹外固定脐产生机械性隐睾。性成熟大鼠90日龄机械诱导单侧隐睾的父权率为83% (n=12)，激素诱导单侧隐睾的父权率为0% （n=10）。Johnsen评分还表明，与机械性隐睾相比，激素性隐睾的睾丸组织发育明显受到抑制。此外，睾丸切除后，激素诱导的隐睾与机械诱导的隐睾相比，父权率的结果明显受到抑制。这些结果提示，引起胎儿期隐睾症的原因不仅会影响大鼠出生后的睾丸发育，还会影响睾丸切除术。

项目成果

Shono T,Suita S: "The effect of the excision of future scrotal skin on testicular descent in neonatal rats.-A new experimental model of cryptorchidism." J Pediatr Surg.30(5). 734-738 (1995)

Shono T，Suita S：“切除未来阴囊皮肤对新生大鼠睾丸下降的影响。-隐睾的新实验模型。”

Shono T: "Extra-abdominal fixation of the gubevnaculum inhibits testiwlar desccut in newbovn rats" J Pediatr Surg. (in press). (1996)

Shono T：“腹外固定脐带可抑制新生大鼠的睾丸剥离”J Pediatr Surg。

生野 猛: "精巣下降におけるホルモンの影響" 医学のあゆみ. 174. 880-882 (1995)

Takeshi Ikuno：“激素对睾丸下降的影响”医学史 174. 880-882 (1995)。

Shono T,Suita S: "Hormonal effect on testicular descent (Japanese)" Igakuno Ayumi. 174(11). 880-882 (1995)

Shono T、Suita S：“荷尔蒙对睾丸下降的影响（日语）” Igakuno Ayumi。

Imajima T,Shono S,Zakaria O,Suita S: "prenatal phthalate causes cryptorchidism postnatally by inducing the transabdominal ascent of the testis in fetal rats." J Pediatr Surg. 32(1). 18-21 (1997)

Imajima T、Shono S、Zakaria O、Suita S：“产前邻苯二甲酸盐通过诱导胎鼠睾丸经腹上升，导致产后隐睾。”