Biologocal analysis of the abnormal urogenityal development in treated with environmental hormone

环境激素治疗泌尿生殖发育异常的生物学分析

• 批准号: 11671772
• 负责人: SHONO Takeshi
• 金额: $ 2.5万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671772/
• 关键词: Phthalate esters; estorgen; antiandrogen; Urogenital anomalies; Undescended testis; MIS m-RNA

Phthalate esters are one of major dndocrine disrupting chemicals, and are known to induce the male reproductive dysfunction urogenital anomalies. We previously reported that prenatally administered phthalate inhibitedtesticular and caused cryptochidism in rats. The mechanism, however, which induce undescended testis remains unknown. In present stydy, we investigate the biological effect of phthalate on testicular descent and the function of the fetal testis. In results, phthalate esters had no estrogenic effect on fetus in vivo, and undescended testis was not induced by antiandrogenic effect of phthalate.Pthalate esters had no effect on the expression of the MIS-mRNA in the testis of newborn rats which were treated with phthlate esters prenatally. Phthalate esters were involved with the inhibition of the testosterone synthesis in fetal rats and low testosterone level was responsible for the hypospadia but not responsible for the undescended testis which is possibly caused by inhibited secretion of insulin-like factor 3 from Leydig cells.

邻苯二甲酸酯是一种重要的内分泌干扰物，可引起男性生殖功能障碍和泌尿生殖系统畸形。我们以前曾报道过，产前给予邻苯二甲酸酯抑制大鼠睾丸，并导致隐睾症。然而，导致隐睾的机制仍不清楚。本研究旨在探讨邻苯二甲酸酯对睾丸下降及胎儿睾丸功能的生物学效应。结果表明，邻苯二甲酸酯类化合物在体内对胎儿无雌激素样作用，邻苯二甲酸酯类化合物的抗雄激素作用不诱导睾丸下降，对产前经邻苯二甲酸酯类化合物处理的新生大鼠睾丸中MIS-mRNA的表达无影响。邻苯二甲酸酯类物质参与了胎鼠睾酮合成的抑制，低睾酮水平与尿道下裂有关，而与睾丸未降无关，睾丸未降可能与睾丸间质细胞分泌胰岛素样因子3受到抑制有关。

项目成果

生野 猛: "精巣下降に及ぼす内分泌撹乱物質について"ホルモンと臨床. 46巻(4). 1135-1140 (1999)

Takeshi Ikuno：“内分泌干扰物对睾丸下降的影响”，激素与临床研究，第 46 卷（1135-1140）。

Shono T: "Time-apecific effects of mono-n-butyl phthalate on the transabdominal descent of the testis in rat fetuses."Br J Urol. 86. 121-125 (2000)

Shono T：“邻苯二甲酸单正丁酯对大鼠胎儿睾丸经腹下降的时间特异性影响。”Br J Urol。

Zakaria O, Shono T, Imajima T, Suita S: "Comparative studies of the fertility and histological development of the contralateral scrotal testes in two different rat models of unilateral cryptorchidism."Pediatr Surg Int.. 16. 498-501 (2000)

Zakaria O、Sono T、Imajima T、Suita S：“两种不同的单侧隐睾大鼠模型中对侧阴囊睾丸的生育力和组织学发育的比较研究。”Pediatr Surg Int.. 16. 498-501 (2000)

Shono T: "Does maternal stress induce a abnormal descent of the testis in prepubertal rats?"Br J Urol International. 84. 353-556 (1999)

Shono T：“母体压力是否会导致青春期前大鼠睾丸异常下降？”Br J Urol International。

Shono T: "Pubertal genitofemoral nerve division induces testicular ascent in adult rats."Journal of Pediat.r Surg.. 34. 346-348 (1999)

Shono T：“青春期生殖股神经分裂诱导成年大鼠睾丸上升。”儿科外科杂志 34. 346-348 (1999)