Osteoarthritis of the TMJ and Mechanical Stress

颞下颌关节骨关节炎和机械应力

• 批准号: 07672110
• 负责人: KUBOKI Takuo
• 金额: $ 1.47万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672110/
• 关键词: Chondrocytes; Mechanical Stress; Cartilage Degradation Factors; Proteoglycan; Cyclic Stress; Matrix Metalloproteinase; 変形性顎関節症; サイトカイン; メタロプロテアーゼ; メタロプロテアーゼインヒビター; 線維芽細胞; 細胞培養; 負荷

Excessive mechanical stress has been proposed as a cause breakdown of the articular cartilage. To investigate the effect of mechanical stress on chondrocyte metabolism, we examined the extracellular matrix synthesis and the gene expression of cartilage degradation factors and growth factors.1.15 kPa cyclic mechanical stress (CTF) caused less increase in proteoglycan (PG) synthesis than 5 kPa (230.3 and 179.1% increase at 5 and 15 kPa)2.5 kPa of CTF caused more decrease in DNA synthesis (50.0 and 30.1% decrease, at 5 and 15 kPa).3.The mean normalized IL-1beta mRNA level against the corresponding control transiently increased during both level of CTF.4.The mean normalized bFGF mRNA level against the corresponding control transiently increased during 15 kPa of CTF,while remarkable change was not observed at 5 kPa of CTF.5.The mean normalized MMP-9 mRNA level against the corresponding control progressively increased during 15 kPa of CTF,while the transient increase was observed during 5 kPa of CTF.6.Mean normalized MMP-2, TIMP-1, agrecan, collagen type II and type X mRNA level against the control did not show remarkable change at the both level of CTF.7.When 15 kPa of CTF was applied, the production level of pro-MMP-9 did not change, but the level of active form MMP-9 was slightly increased, while 5 kPa of CTF was applied, the production level of pro-MMP-9 did not show remarkable change and it was not activated to active form MMP-9. Both level of CTF did not change the production of MMP-2.

过大的机械应力被认为是关节软骨破坏的原因。为了探讨机械应力对软骨细胞代谢的影响，我们检测了细胞外基质合成以及软骨降解因子和生长因子的基因表达。1.15kPa的周期性机械应力引起的蛋白多糖合成的增加幅度小于5kPa(在5和15kPa时分别增加230.3和179.1%)，2.5kpa的周期性机械应力引起的DNA合成的下降幅度更大(50.0%和30.1%)。3.在两个水平的CTF中，对照对照的平均标准化IL-1βmRNA水平在CTF的15 kPa时瞬时升高，而在CTF的5 kPa时无显著变化。在CTF的15 kPa期间，对照对照的平均标准化的MMP-9的mRNA水平逐渐升高，而在CTF的5 kPa时观察到瞬时升高。与对照组相比，在CTF-7作用下，II型胶原和X型胶原的表达水平均无明显变化。当加入15kPA的CTF时，原-基质金属蛋白酶-9的产生水平无明显变化，但活性形式的基质金属蛋白酶-9的表达水平略有增加，而当施加5kPA的细胞外基质纤维时，原-基质金属蛋白酶-9的产生水平无明显变化，未被激活为活性形式的基质金属蛋白酶-9。两种水平的CTF对基质金属蛋白酶-2的产生均无影响。

项目成果

Takuo Fujisawa, Takuo Kuboki et al.: "Induction of interleukins and MMPs in chondrocytes exposed to cyclic mechanical stress" Japanese Journal of Oral Biology. 39 supplement. 122 (1997)

Takuo Fujisawa、Takuo Kuboki 等人：“暴露于循环机械应力的软骨细胞中白细胞介素和 MMP 的诱导”日本口腔生物​​学杂志。

Takuo Fujisawa, Takuo Kuboki, Atsushi Yamashita et al.: "Gene Expression of Cartilage Degradation Factors in Cultured Chondrocytes under Mechanical Stress" 10th Annual Meeting of the Japanese Society for TMJ,program and abstracts. 301 (1997)

Takuo Fujisawa、Takuo Kuboki、Atsushi Yamashita 等人：“机械应力下培养软骨细胞中软骨降解因子的基因表达”日本 TMJ 学会第十届年会，会议议程和摘要。

藤沢 拓生, 窪木 拓男, 山下 敦 他: "培養軟骨細胞における機械的伸展刺激による軟骨破壊因子の遺伝子発現" 第10回日本顎関節学会総会 プログラム・予稿抄録集. 301 (1997)

Takuo Fujisawa、Takuo Kuboki、Atsushi Yamashita 等：“培养软骨细胞中机械拉伸刺激诱导的软骨破坏因子的基因表达”第 10 届日本颞下颌关节学会年会，计划和会议记录，301（1997 年）

藤沢 拓生, 窪木 拓男 他: "メカニカルストレスによる軟骨細胞における各種インターロイキンとのMMPs誘導" 歯科基礎医学会雑誌. 39・抄録集. 122 (1997)

Takuo Fujisawa、Takuo Kuboki 等人：“机械应力导致软骨细胞中各种白细胞介素的诱导”，日本基础牙科医学会杂志 39，摘要 122（1997 年）。