Cytotoxicity testing of dental materials with saliva chemical factors in a culture medium

为了研究含有唾液因子的培养基的细胞可持续组成，使用两种人工唾液（Greenwood、Salivate）和人唾液培养细胞（HEp-2、L-929、RC 2），然后在新鲜培养基中回收细胞。通过向培养基中加入45%的人工唾液，回收了约70%的细胞。然后使用两种细胞系（HEp-2，Gin-1）和组织模型（MATREX），在体外培养基中加入45%的人工唾液和人唾液，通过细胞回收率检查8种金属和3种聚合物假体材料的细胞毒性。钛、银钯合金和热固化树脂无细胞毒性。钴铬合金和锡银合金仅显示轻微的细胞毒性。银铟合金、镍铬合金和镍铜镓合金具有中度细胞毒性作用。铜和两种自固化树脂效果显著。这两种细胞系之间没有差异被发现。目前的研究结果表明，唾液/组织模型对牙科材料的细胞毒性测试可能是有用的，尽管事实上，该组合并不完全支持细胞培养。

To examine the cell sustainable composition of a culture medium containing saliva factors, two kinds of artificial saliva (Greenwood, Salivate) and human saliva were used to cultivate cells (HEp-2, L-929, RC2), followed by cell recovery in a fresh medium. By adding artificial saliva to a medium at 45%, approximately seventy percent of the cells were recovered. Cytotoxicities of eight metallic and three polymeric prosthetic materials were then examined by the cell recovery rate using two cell lines (HEp-2, Gin-1) and tissue model (MATREX) with addition of artificial saliva and human saliva at 45% to medium in vitro. Titanium, silver palladium alloy and heat-cured resin were not cytotoxic. A cobalt chromium alloy, and tin silver alloy showed only slight cytotoxicity. Silver indium alloy, nickel chromium alloy and nickel copper gallium alloy had moderate cytotoxic effects. Copper and two self-curing resins had marked effects. No difference between the two cell lines was found. The present findings suggest that saliva / tissue model couples for cytotoxicity testing of dental materials may be useful, despite the fact that the combination did not fully support cellular cultivation.