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In vitro embryotoxicity testing of dental biomaterials by defferentiation of embryonic stem cells

通过胚胎干细胞分化进行牙科生物材料的体外胚胎毒性测试

基本信息

批准号：
12671913
负责人：
IMAI Koichi
金额：
$ 2.43万
依托单位：
Osaka Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671913/
关键词：
differentiation ES cell monomer metal plasticizer tissue model collagen embryotoxicity 歯科材料 ES 分化 ID50 3T3

项目摘要

The effects of dental biomaterials on the differentiation of embryonic stem cells of the mouse cell line D3 (ES-D3cells) were examined using the embryonic stem cell test (EST), which consists of three parameters, differentiation rate into contracting myocard, 50% cell viability of ES-D3 cells and of Balb/c 3T3 cells, and clone A31 (3T3 cells), which were finally calculated by a designated formula. Of the 24 monomers tested, Bis-GMA, UDMA, Bis-MPEPP, TEGDMA, Bis-GMA(6F), 6-HHMA and MTYA were classified as "weak embryotoxic", and 2.0-EPDMA, 3.0-EPDMA, 4.0-EPDMA, 1.6-ADMA, 1.8-ADMA, 1.10-ADMA, HEPC, Phosmer M, BSNa, EDMABA, GAM, GMA, GMR, NPG, PTSNa and QTX as "non embryotoxic". In the case of metal powder extracted in culture media, it was found that Ag was classfied as "weak embryotoxic", while Cot, Cr, Ni and Pd were classified as "non embryotoxic". On the other hand, in the standard chemicals for atomic absorption spectro photometer, it was found that Cr and Hg ions were classified as "strong embryotoxic", and In, Sn, Sb and V ions were classified as "weak embryotoxic", while Ag, Co, Cu, Ni, Pd and Zn ions were classified as "non embryotoxic". Five plasticizers, dibutyl phthalate, n-butyl benzylphthalate, n-Butyl phthalyl n-butyl glycolate, di-2-ethylhexyl phthalate, di-2-ethylhexyladipate were classified as "weak embryotoxic". The effects of hardened dental biomaterials on the differentiation of ES cells in the Type 1 collagen gel matrix were examined. the differentiation rate was decreased in the order of glassionomer cement, two kinds of light cured composite resins, and amalgam, the most severely damaged to almost zero levelIt is considered that further intensive study on the embryotoxicity of dental biomaterials is needed in addition to the conventional biological aspects from the perspective of biological safety

用胚胎干细胞试验(EST)检测了牙科生物材料对小鼠胚胎干细胞系D3(ES-D3细胞)胚胎干细胞分化的影响，包括向收缩肌细胞的分化率、ES-D3细胞和Balb/c 3T3细胞的50%细胞存活率以及克隆A31(3T3细胞)，并按指定公式计算。在所检测的24种单体中，Bis-GMA、UDMA、Bis-MPEPP、TEGDMA、Bis-GMA(6F)、6-HHMA和Mtya被归类为“弱胚胎毒性”，2.0-EPDMA、3.0-EPDMA、4.0-EPDMA、1.6-ADMA、1.8-ADMA、1.10-ADMA、HepC、Phosmer M、BSNa、EDMABA、GAM、GMA、GMR、NPG、PTSNa和QTX被归类为“非胚胎毒性”。对于在培养液中提取的金属粉末，发现银被归类为“弱胚胎毒性”，而CoT、Cr、Ni和Pd被归类为“无胚胎毒性”。另一方面，在原子吸收光谱仪的标准化学品中，发现铬和汞离子被归类为“强胚胎毒性”，In、Sn、Sb和V离子被归类为“弱胚胎毒性”，而银、钴、铜、镍、钯和锌离子被归类为“非胚胎毒性”。5种增塑剂，邻苯二甲酸二丁酯、邻苯二甲酸正丁酯、邻苯二甲酸正丁酯、邻苯二甲酸二乙基己酯、邻苯二甲酸二乙基己二酯被归类为“弱胚胎毒性”。观察硬化牙科生物材料对ES细胞在I型胶原凝胶基质中分化的影响。分化率由高到低依次为玻璃离子粘固剂、两种光固化复合树脂和银汞合金，损伤最严重的是银汞合金。从生物安全性的角度出发，认为牙科生物材料的胚胎毒性还需要进一步深入研究。