In vitro embryotoxicity testing of dental biomaterials by defferentiation of embryonic stem cells

点击翻译按钮获取中文摘要

The effects of dental biomaterials on the differentiation of embryonic stem cells of the mouse cell line D3 (ES-D3cells) were examined using the embryonic stem cell test (EST), which consists of three parameters, differentiation rate into contracting myocard, 50% cell viability of ES-D3 cells and of Balb/c 3T3 cells, and clone A31 (3T3 cells), which were finally calculated by a designated formula. Of the 24 monomers tested, Bis-GMA, UDMA, Bis-MPEPP, TEGDMA, Bis-GMA(6F), 6-HHMA and MTYA were classified as "weak embryotoxic", and 2.0-EPDMA, 3.0-EPDMA, 4.0-EPDMA, 1.6-ADMA, 1.8-ADMA, 1.10-ADMA, HEPC, Phosmer M, BSNa, EDMABA, GAM, GMA, GMR, NPG, PTSNa and QTX as "non embryotoxic". In the case of metal powder extracted in culture media, it was found that Ag was classfied as "weak embryotoxic", while Cot, Cr, Ni and Pd were classified as "non embryotoxic". On the other hand, in the standard chemicals for atomic absorption spectro photometer, it was found that Cr and Hg ions were classified as "strong embryotoxic", and In, Sn, Sb and V ions were classified as "weak embryotoxic", while Ag, Co, Cu, Ni, Pd and Zn ions were classified as "non embryotoxic". Five plasticizers, dibutyl phthalate, n-butyl benzylphthalate, n-Butyl phthalyl n-butyl glycolate, di-2-ethylhexyl phthalate, di-2-ethylhexyladipate were classified as "weak embryotoxic". The effects of hardened dental biomaterials on the differentiation of ES cells in the Type 1 collagen gel matrix were examined. the differentiation rate was decreased in the order of glassionomer cement, two kinds of light cured composite resins, and amalgam, the most severely damaged to almost zero levelIt is considered that further intensive study on the embryotoxicity of dental biomaterials is needed in addition to the conventional biological aspects from the perspective of biological safety