CALCIFICATION MECHANISM OF THE OSTEOID IN THE PERIODONTAL LIGAMENT ON THE TENSION SIDE DURING TOOTH MOVEMENT.

牙齿移动过程中张力侧牙周韧带骨样的钙化机制。

• 批准号: 07672245
• 负责人: NAKAMURA Yoshiki
• 金额: $ 1.47万
• 依托单位: TSURUMI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672245/
• 关键词: TOOTH MOVEMENT; PERIODONTAL LIGAMENT; OSTEOID; CALCIUM; PHOSPHOROUS; ELEMENTAL ANALYSIS; FLUO-3; UNFIXED,UNDECALCIFIED SECTIONS; 無口定非脱灰切片

Elemental analysis (EPMA) and detection of free Ca^<2+> were performed in the osteoid of the periodontal ligament (tension side) during the tooth movement in rats, using unfixed and undecalcified frozen sections. The uptake of Ca^<45> into the osteoid was examined by autoradiography.Upper first molars of mature male rats were moved seven days, and the upper jaws were fixed in liquid nitrogen. The unfixed and undecalcified frozen sections of the molar periodontal ligament were prepared for histological and histochemical (alkaline phosphatase activity : azo-dye method) observation and also for EPMA.In the detection of free-Ca, fluo-3, a fluorescent free Ca indicator, was injected from the hearts of the rats 60 sec.before freeze fixation by liquid nitrogen.In regard to the Autoradiography, Ca^<45> radioisotope was also injected intraperitoneally 60 min.before perfusion fixation of glutaraldehyde. Then the upper jaws were processed for Autoradiography.A bone surface in the buccal periodont … More al ligament (tension side) showed a wavy configuration, and a considerable amount of osteoid was formed. Intense ALP ase activity was noticed in the osteoid and osteoblastic layr. EPMA on the osteoid revealed extremely high peaks of P and Ca, that dropped a bruptly at first and then declined gradually, as the analyzed site moved away from the edge of the bone. S and K maintained steady peaks throughout the whole analyzed sites. Line analyzes demonstrated constant synchronization for the changes of P and Ca.Green fluorescence, showing the presence free Ca^<2+>, was emitted from the whole periodontal space and pulp cavity. The fluorescence from the osteoid was more intense than that from the periodontal ligament. EPMA data showed higher peak of Ca in the osteoid than in the periodontal ligament.Ca^<45> was deposited deeply in the osteoid of the buccal periodontal ligament (tension side) in 60 min.after the peritoneal injection.These results indicate that the osteoid concentration of P and Ca is elevated in the calcification and their concentration regulated precisely, and also that the Ca was supplied from the capillary, some of which is present in the form of ion in the osteoid. Less

采用不固定、不脱钙冰冻切片，对大鼠牙齿移动过程中牙周膜（张力侧）类骨质进行元素分析（EPMA）和游离Ca^2+检测。移动<45>成年雄性大鼠的上颌第一磨牙7天，并将上颌固定在液氮中。本文对大鼠磨牙牙周膜组织进行了组织学、组织化学（碱性磷酸酶活性：偶氮染料法）和电子探针（EPMA）的观察。在测定游离钙时，在液氮冷冻固定前60秒，从大鼠心脏内注射荧光游离钙指示剂Fluo-3。在放射自显影时，在<45>戊二醛灌注固定前60分钟，腹腔内注射Ca ~（2+）放射性同位素。然后对上颚进行放射自显影处理。 ...更多信息 韧带（张力侧）呈波浪形，并有大量类骨质形成。碱性磷酸酶在骨样层和成骨细胞层有较强的活性。EPMA显示类骨质P和Ca的峰值极高，随着分析部位远离骨边缘，峰值先急剧下降，然后逐渐下降。S和K在整个分析地点保持稳定的峰值。线分析显示P和Ca的变化是同步的，牙周间隙和髓腔均发出绿色荧光，表明游离Ca^2+的存在。类骨质的荧光比牙周膜的荧光更强。EPMA结果显示，类骨质中Ca的峰值高于牙周膜，<45>腹膜内注射后60 min，Ca ~（2+）在颊侧牙周膜（张力侧）的类骨质中沉积较深，说明类骨质中P、Ca的浓度在钙化过程中升高，并有精确的浓度调节，同时说明类骨质中Ca的来源是毛细血管，其中部分以离子形式存在。少

项目成果

NAKAMURA Y., NODA K., TANAKA T.AND KUWAHARA Y.: "THE DISTRIBUTION OF THE EXTRA-CELLULAR FREE Ca^<2+> IN THE PERIODONTAL LIGAMENT AND PERIOSTIUM DURING EXPERIMENTAL TOOTH MOVEMENT USING FLUORESCENT FREE Ca^<2+> INDICATOR FLUO-3" ACTA HISTOCHEMICA ET CYTOCH

NAKAMURA Y.、NODA K.、TANAKA T. 和 KUWAHARA Y.：“使用荧光游离 Ca^<2> 指示剂进行实验性牙齿移动期间牙周韧带和骨膜中细胞外游离 Ca^<2> 的分布