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Gene expression profile of periodontal ligament during tooth movement, using microarray analysis

使用微阵列分析研究牙齿移动过程中牙周膜的基因表达谱

基本信息

批准号：
17592153
负责人：
NAKAMURA Yoshiki
金额：
$ 2.11万
依托单位：
Tsurumi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Gene expression profile of the periodontal ligament (PDL) was examined in rat maxillary first molar during tooth movement. Rat maxillary first molars were moved lingually with fixed appliance for 6 hours and 5 days, and unfixed, undecalcified frozen sections was prepared from the molar areas. The periodontal ligament was microdissected from the sections and the RNA was isolated from the sample, and analyzed using cDNA microarray. The data from the microarray was compared with the data from the control (data of periodontal ligament without tooth movement).The quality and quantity of the sample collected with laser capture microdissection was enough for microarray analysis. 6 hours after initiation of tooth movement, in the extended zone of the PDL, 2506 genes were expressed 2 fold higher than those in the control and 2722 genes were expressed half lower than the control. In the compressed zone of the PDL, 2960 genes were expressed 2 fold higher than those in the control and 2978 genes were expressed half lower than the control. 5 days after initiation of tooth movement, in the extended zone of the PDL, 3762 genes were expressed 2 fold higher than those in the control and 2722 genes were expressed half lower than the control. In the compressed zone of the PDL, 4239 genes were expressed 2 fold higher than those in the control and 2849 genes were expressed half lower than the control. The genes related to osteogenesis were up-regulated in the sample of the extended zone of PDL, and the genes related to apoptosis and bone resorption were also up-regulated in the sample of the compressed zone of PDL.These results suggest that the PDL cells change their gene expression to adapt to new environment caused by tooth movement, and these change make it possible to move the tooth in the alveolar bone.

在牙齿移动期间，检查了大鼠上颌第一磨牙的牙周膜（PDL）的基因表达谱。用固定矫治器舌侧移动大鼠上颌第一磨牙6小时和5天，并从磨牙区域制备未固定、未脱钙的冰冻切片。从切片中显微解剖牙周韧带，从样品中分离 RNA，并使用 cDNA 微阵列进行分析。将来自微阵列的数据与来自对照的数据（没有牙齿移动的牙周膜数据）进行比较。通过激光捕获显微切割收集的样本的质量和数量足以用于微阵列分析。牙齿移动开始后6小时，在PDL的延伸区，2506个基因的表达比对照高2倍，2722个基因的表达比对照低一半。在PDL的压缩区，2960个基因的表达比对照高2倍，2978个基因的表达比对照低一半。牙齿移动开始后5天，在PDL的延伸区，3762个基因的表达比对照高2倍，2722个基因的表达比对照低一半。在PDL的压缩区，4239个基因的表达比对照高2倍，2849个基因的表达比对照低一半。 PDL延伸区样本中与成骨相关的基因上调，PDL压缩区样本中与细胞凋亡和骨吸收相关的基因也上调。这些结果表明，PDL细胞改变其基因表达以适应牙齿移动引起的新环境，这些变化使得牙齿在牙槽骨中移动成为可能。