The establishment of artificial indoor breeding systems for the Japanese monkey

日本猴室内人工繁育系统的建立

• 批准号: 07680914
• 负责人: TORII Ryuzo
• 金额: $ 1.41万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680914/
• 关键词: Japanese monkey; indoor breeding; steroid hormone; artificial insemination; IVF; ICSI; embryo transfer; 人工繁殖

For the past two years we have investigated methods of artificial indoor breeding in the Japanese monkey. Our goal is to make the Japanese monkey, the seasonal breeder, as good experimental animal for biomedical researches which can breed throughout the year under laboratory conditions. Results obtained in the present investigation are as follows. 1) Results of whole year observations of serum hormone level, ovarian morphology, and testicular histology showed clear seasonal changes, and sexual function in both sexes declined distinctly in summer. 2) During fertile season in the winter, appropriate timing of ovulation was verified by laparascopy and sperm collected by electro-stimulation were introduced into the uterus through the vagina. The fertilization resulted in parturition was achieved in 3 out of 6 cases. 3) During infertile season in the summer, the ovulation was chemically induced and the artificial insemination attempted by the same methods as in the winter breeding period. H … More owever, pregnancy and partrurition were not achieved in all of 6 cases. 4) A sustained-release formation of a GnRH agonist was utilized maintain the sexual function during summer, but sufficient maintenance of activity was not achieved. 5) IVF (in vitro fertilization) was tested using sperms and mature ova acquired through electro-stimulation and chemically induced ovulation, respectively. Fertilization and two to six cleavages of the zygote were confirmed in 12 out of 16 cases. 6) Prosperous methods for preservative solution and freezing of sperms available to artificial insemination were established. 7) Fertilization and cleavage of the zygote during the summer sterile period were also achieved by ICSI (intra cytoplasmic sperm injection) using sperms and ova acquired through electro-stimulation and chemically induced ovulation, respectively. Fertilization and two to six cleavages of the zygote were confirmed in all of 4 cases. 8) On an attempt of embryo transfer (ET), embryos acquired by the above mentioned IVF and ICSI procedures were transferred to the oviduct of females under laparascopic observations but none of those became pregnant.As shown above, introduction of sperm into the vagina, being a convenient method of artificial insemination, is not an efficient method for breeding. Now, IVF,ICSI and ET investigated in the present study are expected to become good methods for the year round indoor breeding, through further studies for implantation and maintenence of pregnancies. Less

在过去的两年里，我们研究了日本猴的人工室内繁殖方法。我们的目标是使日本猴，季节性繁殖者，作为生物医学研究的良好实验动物，可以在实验室条件下全年繁殖。本研究获得的结果如下。1)全年观察血清激素水平、卵巢形态和睾丸组织学的结果显示出明显的季节性变化，两性的性功能在夏季明显下降。2)在冬季的生育季节，通过腹腔镜检查验证排卵的适当时间，并通过电刺激收集的精子通过阴道引入子宫。6例中3例受精成功分娩。3)在夏季不育季节，采用化学诱导排卵，并尝试用与冬季繁殖期相同的方法进行人工授精。H ...更多信息 6例均未成功妊娠分娩。4)在夏季使用GnRH激动剂的缓释形式维持性功能，但未达到足够的活性维持。5)分别使用通过电刺激和化学诱导排卵获得的精子和成熟卵子进行IVF（体外受精）试验。16例中有12例证实受精和2 ~ 6次受精卵卵裂。6)建立了适用于人工授精的精子保存液和冷冻方法。7)在夏季不育期受精和卵裂的受精卵也实现了ICSI（胞质内精子注射）使用精子和卵子获得通过电刺激和化学诱导排卵，分别。4例均证实受精，受精卵卵裂2 ~ 6次。8)在尝试胚胎移植（ET）时，通过上述IVF和ICSI程序获得的胚胎在腹腔镜观察下被移植到雌性的输卵管中，但没有一个怀孕。如上所述，将精子引入阴道是一种方便的人工授精方法，但不是一种有效的繁殖方法。目前，IVF、ICSI和ET有望通过进一步的研究，成为全年室内育种的良好方法。少