Research for the establishment of artificial indoor breeding systems for the Japanese monkey using developmental technology

利用发育技术建立日本猴人工室内饲养系统的研究

• 批准号: 11558097
• 负责人: TORII Ryuzo
• 金额: $ 7.74万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11558097/
• 关键词: JAPANESE MONKEY; IN VITRO FERTILIZATION; ICSI(1NTRACYTOPLASMIC SPERM INJECTION; IN VITRO CULTURE; EMBRYONIK STEM CELL; CRYOPRESERVATION; 顕微受精; 凍結保存; 体外授精; 顕微授精-胚移植

Results of four year (1999-2002) investigation of embryo transfer (ET) in the Japanese monkey (Macaca fuscata) in association with reproductive engineering techniques such as artificial insemination, in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), in vitro maturation (IVM), etc., are as follows :(1) Established method for retrieval of mature ova from eCG(PMSG), hMG and FSH stimulated ovaries using laparoscopy.(2) Established IVF-ET method for transfer of 2-4 cell embryo to the fallopian tube under laparoscopic observation.(3) Successfully produced world's first IVF-ET pregnancy and live birth in the Japanese monkey using this method.(4) Established liquid nitrogen cryopreservation method for sperm collected either by electrostimulation or directly from euthanized subjects' epididymis.(5) Established cryopreserved sperm ICSI-ET method. Successfully produced world's first ICSI-ET crab eating macaque (Macaca fascicularis) fetus using this method.(6) Established zygo … More te IVM method. Successfully established world's first embryonic stem cell (ES cell) in the Japanese monkey and the crab eating macaque using this method.(7) Breakthrough in liquid nitrogen zygote cryopreservation close.(8) As of today nine artificially produced individuals weaned. As of March, 2002, 28 artificial impregnations confirmed.(9) All artificially produced individuals confirmed negative for salmonella, shigella, tuberculosis, internal parasites, external parasites, B-virus, HSV-1, SIV, SRV, SVV, filovirus, STLV, and measles antibodies. Production of specific pathogen free (SPF) individual now possible.(10) Success producing a ISCI-ET Japanese monkey remains elusive : must identify reason why.Following methods now established in the Japanese monkey : ova collection from stimulated ovary using laparoscopy ; ICSI ; fallopian tube ET using laparoscopy ; sperm and fertilized zygote cryopreservation. All preparations necessary for planned, indoor breeding of the Japanese monkey using these methods believed complete. Less

对日本猕猴（Macaca fuscata）胚胎移植（ET）与生殖工程技术（如人工授精、体外受精（IVF）、卵胞浆内单精子注射（ICSI）、体外成熟（IVM）等）的4年（1999-2002）研究结果，（1）建立了腹腔镜下从eCG（PMSG）、hMG和FSH刺激的卵巢中提取成熟卵子的方法。(2)建立了腹腔镜下2-4细胞胚胎输卵管内移植的体外受精-胚胎移植方法。(3)利用该方法在日本猴子中成功实现了世界首例IVF-ET妊娠和活产。(4)建立了电刺激或直接从安乐死受试者附睾采集精子的液氮冷冻保存方法。(5)建立了冷冻精子ICSI-ET方法。利用该方法成功生产了世界上第一例ICSI-ET食蟹猕猴（Macaca fascicularis）胎儿。(6)建立Zygo ...更多信息 特IVM方法。利用该方法在日本猴和食蟹猕猴体内成功建立了世界上第一个胚胎干细胞（ES细胞）。(7)受精卵液氮冷冻保存接近突破。(8)到今天为止，9只人工生产的个体断奶了。截至2002年3月，确认了28个人工流产。(9)所有人工生产的个体均确认沙门氏菌、志贺氏菌、结核病、体内寄生虫、体外寄生虫、B病毒、HSV-1、SIV、SRV、SVV、丝状病毒、STLV和麻疹抗体阴性。现在可以生产无特定病原体（SPF）的个体。(10)成功生产ISCI-ET日本猴仍然难以捉摸：必须找出原因。以下方法现在建立在日本猴：卵子收集从刺激卵巢使用腹腔镜; ICSI ;输卵管ET使用腹腔镜;精子和受精卵冷冻保存。使用这些方法对日本猴进行有计划的室内繁殖所需的所有准备工作都已完成。少

项目成果

Torii R, Fujinami N., Hosoi Y, Takenoshita Y, Iritani A: "First successful birth of the Cynomolgus monkey (Macaca fascicularis) by intra-cytoplasmic sperm injection and embryo-transfer(ICSI-ET)"Exp. Anim.. 50・3. 63-63 (2001)

Torii R, Fujinami N., Hosoi Y, Takenoshita Y, Iritani A：“首次通过胞浆内精子注射和胚胎移植 (ICSI-ET) 成功诞生食蟹猴 (Macaca fasciculis)”Exp. 50・3。63-63（2001）

Torii R, Takada T, Suzuki Y, Kadota N, Kobayashi K, Kondo Y, Nito S, Kimura H, Hosoi, Y, Iritani A: "Establishment of pluripotent cynomolgus monkey ES Cell line expressing GFP"Exp. Anim. 51・3. 80-80 (2002)

Torii R、Takada T、Suzuki Y、Kadota N、Kobayashi K、Kondo Y、Nito S、Kimura H、Hosoi、Y、Iritani A：“表达 GFP 的多能食蟹猴 ES 细胞系的建立”Exp. 51・3 .80-80 (2002)

Torii,R., Takada,T., Suzuki,Y., Kadota,N., Kobayashi,K., Kondo,Y., Nito S., Kimura H., Hosoi,Y. & Iritani,A.: "Establishment of pluripotent cynomolgus monkey ES Cell line expressing GFP"Exp.Anim.. 51(3). S80 (2002)

鸟井，R.，高田，T.，铃木，Y.，门田，N.，小林，K.，近藤，Y.，尼托S.，木村H.，细井，Y.

鳥居隆三: "サル(ニホンザル、カニクイザル)ES細胞株の樹立とこれからの展開"関西実験動物研究会報. 22. 55-62 (2001)

鸟居龙三：“猴（日本猕猴、食蟹猴）ES细胞系的建立和未来发展”《关西实验动物研究公报》22. 55-62（2001）。

Hosoi Y, Torii R, Fujinami N, Matsumoto K, Saeki K, Iritani A: "Fertilization by intracytoplasmic sperm injection and subsequent embryo development in vitro to blastocysts in Japanese monkey (macaca fuscata)"J. Mammakian Ova Research. 20・1. (2003)

Hosoi Y、Torii R、Fujinami N、Matsumoto K、Saeki K、Iritani A：“日本猴（猕猴）胞浆内精子注射受精和随后的胚胎体外发育至囊胚”J. Mammakian Ova Research 20・1。 (2003)