神経栄養因子による小児期脳障害の治療に関する基礎的研究

神经营养因子治疗儿童脑部疾病的基础研究

• 批准号: 07770610
• 负责人: 今井 祐之
• 金额: $ 0.58万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07770610/
• 关键词: 神経栄養因子; 脳障害; 神経細胞

研究目的:周産期脳障害は医療技術の進歩により、その予防・早期診断が可能になりつつある。しかし、再生(分裂)能力のない神経細胞にいったん加わった傷害に対して、その構造・機能を積極的に回復させる治療法は未だない。本研究では神経成長因子(NGF)およびその受容体(NGFR)からなるカスケード(NGF/NGFRカスケード)を用いた周産期脳障害に対する新たなる治療法の開発を目指すことを目的とする。1.結果:ヒトNGFRをコードする遺伝子trkA cDNAをinvitroにおいて未分化神経細胞株(HTLA230)内へ導入した。FACS解析において約70%の細胞で細胞表面上にtrkAを認めた。発現したNGF receptorは、NGF(50ng/ml)投与に反応してimmediate-early resonseとしてのc-fosの一過性発現を呈した。またトランスフェクタントに対するNGFの長期投与は未分化細胞のネットワーク形成を伴う形態学的分化と神経特異的遺伝子の発現を特徴とするlate responseを示した。2.さらに遺伝子導入された未分化神経細胞は、in vivoにおいてもNGF投与(250ng)に反応して未分化細胞を分化型神経細胞へと誘導した。以上の結果は未分化神経細胞表面上に人為的に構築された機能的NGFRは未分化神経細胞をin vitroおよびin vivoの両者において分化誘導することを意味する。今後はNGFR cDNAをラットの上頚神経節(SCG)内に直接注入した後、SCGの軸索切断を行った後NGF投与を行い、in vivoにおいて人為的に構築されたNGF/NGFRカスケードが神経傷害修復に促進機能を有するか否かを検討する。またin vivoでの神経細胞内への安全かつ有効な遺伝子導入のためのベクターの開発も必要となる。

The purpose of the study is to prevent the development of medical technology during the weekly period, and to prevent early failure. The ability to recover, regenerate (divide) is very important, and the machine has been able to actively treat the disease and cure the disease. In this study, the growth factor (NGF), the growth factor, the growth factor, the growth factor 1. Results: the undifferentiated mycelium cell line (HTLA230) was isolated from the undifferentiated cell line (HTLA230) of the undifferentiated NGFR cell line (HTLA230), which was cloned into the cell line (HTLA230). FACS analysis showed that there were trkA fragments on the surface of the cells. The result of NGF receptor, NGF (50ng/ml) investment and anti-immediate-early resonse response is negative as soon as c-fos is found to be ineffective. This is the result of a long-term study of undifferentiated cells, which is associated with the differentiation of morphology. the results show that there is a long-term relationship between NGF and undifferentiated cells, which is associated with the differentiation of physiology. two。 The cells of the undifferentiated gods were introduced into the cells of the undifferentiated gods, the NGF of the undifferentiated cells of the in vivo cells, and the guidance of the differentiated cells of the undifferentiated cells. The results above showed that on the surface of the undifferentiated cells, the NGFR cells of the undifferentiated myocytes, the undifferentiated cells of the human brain, the cells of the undifferentiated cells, the cells of the undifferentiated cells, the cells of the in vivo cells, the cells of the undifferentiated cells, the cells of the undifferentiated cells, the cells of the in vivo cells, the cells of the undifferentiated cells, the undifferentiated, the undifferentiated, the undifferentiated. In the future, after the SCG is directly injected into the SCG, the NGF is invested in the bank after the SCG cable is cut off, and the in vivo is operated by the operator. The machine is capable of improving the performance of the machine. During the in vivo period, there are many people in the cell who need to pay attention to the necessary information.