Cloning of Platelet aggregation factors from tumor cells
从肿瘤细胞克隆血小板聚集因子
基本信息
- 批准号:08045062
- 负责人:
- 金额:$ 2.94万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for international Scientific Research
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
One of the main causes of death of cancer patients is thrombosis. This is probably because cancer cells activate platelet aggregation and blood coagulation. Furthermore, it is fairly well accepted that above phenomena might be responsible for metastasis of cancer. In the initial year of our research program we did following experiments. We found using a neuroblastoma cell GOTO that platelet aggregation was induced upon addition of this cell to platelet-rich plasma. We also found that the addition of GOTO cells to normal plasma activated Factor X and it in turn activated prothrombin and produced thrombin. We then found that both activities, the activation of Factor X and prothrombin, disappeared upon differentiation of GOTO cells to Shwanian-like cells. Based on the results obtained in the initial year, we did subtraction cloning and production of monoclonal antibodies in the second year with the aim of elucidating the underlining mechanism of thrombin production by GOTO cells in molecular terms. In the final year we are looking into the specific binding of Factor X to the cells to carry out an expression cloning. As far as the production of monoclonal antibodies is concerned, we have been able to clone hybridomas directly on soft : agar plates and we have obtained several clones with the desired properties. We hope the time will soon come to start the actual cloning experiment with these antibodies. On the other hand we have made much improvement as to the purification of highly polar lipid, probably glycolipid, which is the antigen for the monoclonal antibody we established last year which specifically recognized the surface of neuroblastoma cells. We hope we could do NMR and mass spectrometric analyses to elucidate the chemical structure of this interesting lipid. We will chemically synthesize the lipid and elucidate the biological function of this lipid.
血栓形成是癌症患者死亡的主要原因之一。这可能是因为癌细胞激活了血小板聚集和血液凝固。此外,人们普遍认为上述现象可能与癌症的转移有关。在我们研究计划的最初一年,我们做了以下实验。我们使用神经母细胞瘤细胞GOTO发现,当将该细胞加入富含血小板的血浆中时,可以诱导血小板聚集。我们还发现,在正常血浆中加入Goto细胞可激活凝血因子X,进而激活凝血酶原并产生凝血酶。然后我们发现,当Goto细胞分化为Shwanian样细胞时,这两种活性,即凝血因子X和凝血酶原的激活都消失了。在最初一年的基础上,我们在第二年进行了减法克隆和单抗的制备,目的是从分子水平上阐明GoTO细胞产生凝血酶的基本机制。在最后一年,我们正在研究因子X与细胞的特定结合,以进行表达克隆。在生产单抗方面,我们已经能够直接在软琼脂平板上克隆杂交瘤,并获得了几个具有所需特性的克隆。我们希望很快就能开始用这些抗体进行实际的克隆实验。另一方面,我们在高极性脂类的纯化方面取得了很大的进步,可能是糖脂,这是我们去年建立的特异性识别神经母细胞瘤细胞表面的单抗的抗原。我们希望我们能进行核磁共振和质谱分析来阐明这种有趣的脂类的化学结构。我们将用化学方法合成这种脂类,并阐明这种脂类的生物学功能。
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
H.Inuyama: "Factor-X-dependent thrombiN generating activity on a neuroblastoma cell and their disapperrance upon differentiation" Journal of Cellular physiology. 173. 406-414 (1997)
H.Inuyama:“神经母细胞瘤细胞上 X 因子依赖性血栓生成活性及其分化后的消失”《细胞生理学杂志》。
- DOI:
- 发表时间:
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- 影响因子:0
- 作者:
- 通讯作者:
S.Ueki: "12-Hydroxy-5Z,8Z,10E,14Z,eicosatetraenoic acid (12-HETE) stimulates cAMP production in normal human fibroblasts" Journal of Cellular Physiology. 178. 63-68 (1999)
S.Ueki:“12-羟基-5Z、8Z、10E、14Z、二十碳四烯酸 (12-HETE) 刺激正常人成纤维细胞中 cAMP 的产生”《细胞生理学杂志》。
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- 影响因子:0
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- 通讯作者:
A.Kushida: "Screening of hybridoma clones producing antibodie against plasma membrane-associated materials" Hybridoma. 17. 209-213 (1998)
A.Kushida:“筛选产生针对质膜相关材料的抗体的杂交瘤克隆”杂交瘤。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
A.Kushida: "Screening of hybridoma clones producing antibodies against plasma membrane-associated materials" Hybridoma. (印刷中).
A.Kushida:“筛选产生针对质膜相关材料的抗体的杂交瘤克隆”杂交瘤(正在出版)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
H.Inuyama: "Factor-X-dependent thrombin generating activity on a neruroblastoma cell and their disappearance upon differentiation" Journal of Cellular Physiology. 173. 406-414 (1997)
H.Inuyama:“神经母细胞瘤细胞上 X 因子依赖性凝血酶生成活性及其在分化时的消失”《细胞生理学杂志》。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
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SAITO Yuji其他文献
ESTIMATION OF SKIN-FRICTION FIELD ON A BACKWARD-FACING STEP (BFS) MODEL FROM TSP IMAGES USING LINEAR-LEAST-SQUARES METHOD
使用线性最小二乘法根据 TSP 图像估计向后步 (BFS) 模型上的皮肤摩擦场
- DOI:
- 发表时间:
2019 - 期刊:
- 影响因子:0
- 作者:
Jingqi Ma;Taekjin Lee;Chiaki Kawase;Lin Chen;SAITO Yuji;Taku Nonomura;Keisuke Asai - 通讯作者:
Keisuke Asai
線形最小二乗法を用いた感温塗料における表面摩擦応力場の推定
使用线性最小二乘法估计温敏涂料表面摩擦应力场
- DOI:
- 发表时间:
2019 - 期刊:
- 影响因子:0
- 作者:
Jingqi Ma;Taekjin Lee;Chiaki Kawase;Lin Chen;SAITO Yuji;Taku Nonomura;Keisuke Asai;馬 敬旗,李 澤辰,河瀬 千暁,陳 林,齋藤 勇士,野々村 拓,浅井 圭介 - 通讯作者:
馬 敬旗,李 澤辰,河瀬 千暁,陳 林,齋藤 勇士,野々村 拓,浅井 圭介
低電圧で駆動可能な高集積プラズマアクチュエータ
高度集成的等离子体执行器,可在低电压下驱动
- DOI:
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2021 - 期刊:
- 影响因子:0
- 作者:
AOKI Rui;FUJIMURA Ikuhiro;HANDA Taro;LEE Chungil;OZAWA Yuta;SAITO Yuji;NONOMURA Taku;ASAI Keisuke;佐藤慎太郎 - 通讯作者:
佐藤慎太郎
Feasibility Study of Controlling Supersonic Boundary-layer Flows Using Jets Flapping at Several Tens of Kilohertz
数十千赫兹射流扑动控制超音速边界层流的可行性研究
- DOI:
10.2322/tjsass.65.221 - 发表时间:
2022 - 期刊:
- 影响因子:1.1
- 作者:
AOKI Rui;FUJIMURA Ikuhiro;HANDA Taro;LEE Chungil;OZAWA Yuta;SAITO Yuji;NONOMURA Taku;ASAI Keisuke - 通讯作者:
ASAI Keisuke
自在な流れ形成に向けた印刷技術によるDBDプラズマアクチュエータの開発
使用印刷技术开发 DBD 等离子体致动器以实现自由流动
- DOI:
- 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
OZAWA Yuta;NONOMURA Taku;SAITO Yuji;ASAI Keisuke;新浜 優貴,黒木 太司,餘利野 直人,佐々木 豊,造賀 芳文;榎戸智輝,佐藤慎太郎,大西直文 - 通讯作者:
榎戸智輝,佐藤慎太郎,大西直文
SAITO Yuji的其他文献
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{{ truncateString('SAITO Yuji', 18)}}的其他基金
接合部周辺の形状変形が少ない輻射熱接合に関する研究
接头周围小变形辐射热粘合研究
- 批准号:
19H00273 - 财政年份:2019
- 资助金额:
$ 2.94万 - 项目类别:
Grant-in-Aid for Encouragement of Scientists
Structure and Function of a Ganglioside found Exclusively on the Surface of Neuroblastoma cells
仅在神经母细胞瘤细胞表面发现的神经节苷脂的结构和功能
- 批准号:
11680602 - 财政年份:1999
- 资助金额:
$ 2.94万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Structure-function Studies On A Cancer Cell-specific Cell Adhesion Protein
癌细胞特异性细胞粘附蛋白的结构功能研究
- 批准号:
06454644 - 财政年份:1994
- 资助金额:
$ 2.94万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of functional domains within adhesive proteins with the aim of controlling the interaction between cells and collagens
分析粘附蛋白内的功能域,旨在控制细胞和胶原蛋白之间的相互作用
- 批准号:
03454498 - 财政年份:1991
- 资助金额:
$ 2.94万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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