Preparations of Cast Film of Aligned DNA Doule Srtands

对齐DNA双链流延膜的制备

• 批准号: 08455437
• 负责人: OKAHATA Yoshio
• 金额: $ 4.74万
• 依托单位: TOKYO INSTITUTE OF TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08455437/
• 关键词: DNA; Langmuir-Blodgett Film; Aligned thin film; Cast Film; DNA-lipid complex

We have prepared DNA-lipid complex by mixing both aqueous solutions of DNA from sermon testes and cationic lipid molecules. The precipitated DNA-lipid complex is soluble in most of organic media, bur not in aqueous solutions. When chloroform solution of DNA-lipid complex was cast on a glass plate, a transparent, physically strong film was obtained. Following results were obtained.1)A cast film was stretched in one direction, the DNA-aligned film was obtained, which was confirmed by X-rya diffraction analyses.2)When the DNA-lipid cast film was soaked in aqueous solution of ethidium bromide, the film was colored to red due to the intercalated ethidium bromide dye molecules. Orientation of intercalated dye molecules could be confirmed by polarized absorption spectra of the film.3)DNA-aligned cast film showed a large anisotropic behavior of conductivity. The electrostatic conductivity was observed along the DNA strands in the film, but not perpendicular to the DNA strands.

我们通过混合来自布道睾丸的DNA水溶液和阳离子脂质分子来制备DNA-脂质复合物。沉淀的DNA-脂质复合物可溶于大多数有机介质，但不溶于水溶液。当DNA-脂质复合物的氯仿溶液流延在玻璃板上时，获得透明的、物理上坚固的膜。结果表明：1）将铸膜单向拉伸，得到了DNA取向膜，并通过X射线衍射分析证实了这一点; 2）将DNA-脂质铸膜浸泡在溴化乙锭水溶液中，由于溴化乙锭染料分子的插入，膜被染成红色。薄膜的偏振吸收光谱证实了染料分子的取向。3）DNA取向的流延膜表现出很大的电导率各向异性。沿着膜中的DNA链观察到静电导电性，但不垂直于DNA链。

项目成果

Y.Okahata,T.Kobayasi,and K.Tanaka: "Orientation of DNA Double Strands in a Langmuir-Blodgett Film" Langmuir. 12. 1326-1330 (1996)

Y.Okahata、T.Kobayasi 和 K.Tanaka：“Langmuir-Blodgett 薄膜中 DNA 双链的方向”Langmuir。

R.Takei, K-H.Park, H.Ise, A.Maruyama, Y.Ebara, Y.Okahata, and T.Akaike: "Coated Antireceptor Antibody as an Extracellular Matrix for Liver Tissue Engineering" Tissue Enginnering. 3. 281-288 (1997)

R.Takei、K-H.Park、H.Ise、A.Maruyama、Y.Ebara、Y.Okahata 和 T.Akaike：“涂层抗受体抗体作为肝组织工程的细胞外基质”组织工程。

Y.Okahata and K.Tanaka: "Oriented Thin Films of a DNA-lipid Complex" Thin Solid Films. 284/285. 6-8 (1996)

Y.Okahata 和 K.Tanaka：“DNA-脂质复合物的定向薄膜”固体薄膜。

Y.Ebara, Y.Okahata, and T.Akaike: "Coated Antireceptor Antibody as an Extracellular Matrix for Liver Tissue Engineering" Tissue Engineering. 3. 281-288 (1997)

Y.Ebara、Y.Okahata 和 T.Akaike：“涂层抗受体抗体作为肝组织工程的细胞外基质”组织工程。

K.Matsuura, Y.Ebara, and Y.Okahata: "Gas Phase Molecular Recognition on Nucleobose Monolayers Immobilized on a Highly Sensitive Quartz-Crystal Microbalance" Langmuir. 13. 814-820 (1997)

K.Matsuura、Y.Ebara 和 Y.Okahata：“固定在高灵敏度石英晶体微天平上的核糖单层的气相分子识别”Langmuir。