Study of A Lipid-coated Enzyme acts as a Catalyst in Organic Media

脂质包被酶在有机介质中作为催化剂的研究

• 批准号: 08555202
• 负责人: OKAHATA Yoshio
• 金额: $ 7.17万
• 依托单位: TOKYO INSTITUTE OF TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08555202/
• 关键词: Lipid-coated enzyme; Enzyme reaction in Organic Media; Enantioselective reactions; Lipase; Esterification

We have prepared a lipid-coated enzyme, in which enzyme surfaces were covered with a monolayer of dialkyl amphiphiles and alkyl chains could solubilize enzymes in organic media. The lipid-coated enzymes can be solubilized in most of organic solution and act as an efficient catalyst. Following results were obtained.1) A lipid-coated enzyme was obtained by mixing both aqueous solutions of enzyme and amphiphiles, and obtained precipitated are soluble only in organic media, but not in aqueous solutions.2) The lipid-coated lipase can catalyze the enantioselective esterification (reverse reaction of hydrolysis) in isooctane. When the lipid-coated galactosidase was prepared, it acts as an efficient catalyst as transglycosylation in isopropyl ether.3)We believe the lipid-coating system can be applied for most of enzymes to solubilize them in organic media without denaturation.

我们已经准备了一种脂质涂层的酶，其中酶表面覆盖有二烷基两亲物的单层，烷基链可以在有机培养基中溶解酶。可以在大多数有机溶液中溶解脂质涂层的酶，并充当有效的催化剂。 1）通过混合酶和两亲物的水溶液可获得脂质涂层的酶，并获得的沉淀仅在有机培养基中溶液。当制备脂质涂层的半乳糖苷酶时，它在异丙基醚中充当有效的催化剂，作为丙糖基化。3）我们相信，可以将脂质涂层系统应用于大多数酶，以使它们在有机培养基中溶解它们而无需脱生。

项目成果

Y.Okahata and T.Mori: "Effective Transgalactosylation catalysed by a lipid-coated b-D-galactosidase in organic solvents" J.Chem.Soc.,Perkin Trans 1. 2861-2866 (1996)

Y.Okahata 和 T.Mori：“有机溶剂中脂质包被的 b-D-半乳糖苷酶催化的有效转半乳糖基化”J.Chem.Soc.，Perkin Trans 1. 2861-2866 (1996)

T. Mori and Y. Okahata: "A Variety of Lipid-coated Glycoside hydrolases as Effective Glycosyl Transfer Catalyses in Homogeneous Organic Solvents" Tetrahedron Lett.38. 1971-1974 (1997)

T. Mori 和 Y. Okahata：“多种脂质包被的糖苷水解酶作为均质有机溶剂中有效的糖基转移催化剂”Tetrahedron Lett.38。

Y.Okahata and T.Mori: "Lipid-coated Enzymes as Efficient Catalysts in Organic media" Trends in Biotechnolgy. 15. 50-54 (1997)

Y.Okahata 和 T.Mori：“脂质包被酶作为有机介质中的高效催化剂”生物技术趋势。

森 俊明、 岡畑恵雄: "日本応用糖質科学会誌" 脂質修飾法を用いる糖加水分解酵素の改変-有機溶媒中での配糖化反応-, 342 (1997)

森俊明、冈田庆男：《日本应用糖科学会杂志》利用脂质修饰法修饰糖水解酶 - 有机溶剂中的糖基化反应 -，342（1997）

Y.Okahata and T.Mori: "Catalytic Activity of Lipid-coated Enzymes in Organic Media" Proc.Japan Acad.73,Ser.B. 210-214 (1997)

Y.Okahata 和 T.Mori：“有机介质中脂质包被酶的催化活性”Proc.Japan Acad.73，Ser.B。