Development of 96-type and multi-type quartz-crystal microbalance

96型及多型石英晶体微天平的研制

• 批准号: 13558079
• 负责人: OKAHATA Yoshio
• 金额: $ 8.64万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13558079/
• 关键词: DNA hybridization; quartz-crystal microbalance; multi-sensor; 4-hole type QCM; QCM; DNA; たんぱく質

The purpose of this research is to develop a multi-type (96 hole)quartz-crystal microbalance (QCM In this paper, we introduce a 27-MHz quartz-crystal microbalance (QCM) as a new tool to detect DNA-protein/peptide interactions in an aqueous buffer solution. From the time course of the frequency decrease (mass increase), the binding amount (Δm), association constant (Ka), binding and dissociation rate constant (k1 and k1) can be obtained. When dsDNA was immobilized on a QCM, binding kinetics of antibiotics, a Zinc-finger type peptide, a restriction enzyme, and a polymerase to DNA strands could be obtained in aqueous buffer solution. The 27-MHz QCM is highly sensitive and quantitative enough to detect various molecular interactions on DNA double strands.

本研究的目的是开发一种多类型（96孔）石英晶体微天平（QCM）在本文中，我们介绍了27-MHz的石英晶体微天平（QCM）作为一种新的工具来检测DNA-蛋白质/肽在水缓冲溶液中的相互作用。根据频率降低（质量增加）的时间过程，可以获得结合量（Δm）、缔合常数（Ka）、结合和解离速率常数（k1和k1）。当双链DNA固定在QCM上时，抗生素、锌指型肽、限制性内切酶和聚合酶与DNA链的结合动力学可以在水性缓冲溶液中获得。27-MHz QCM具有高灵敏度和定量性，足以检测DNA双链上的各种分子相互作用。

项目成果

H.Furusawa, Y.Kitamura, N.Hagiwara, T.Turimoto, Y.Okahata: "Binding Kinetics of the Toroidal-Shaped PCNA to DNA Strands on a 27 MHz Quartz-Crystal Microbalance"ChemPhysChem.. No.5. 446-448 (2002)

H.Furusawa、Y.Kitamura、N.Hagiwara、T.Turimoto、Y.Okahata：“27 MHz 石英晶体微天平上环形 PCNA 与 DNA 链的结合动力学”ChemPhysChem.. No.5。

M.Tanaka, A.Mochizuki, T.Motomura, K.Shimura, M.Onishi, Y.Okahata: "In Situ Studies on Protein Adsorption onto a Poly(2-methoxyethylacrylate) Surface by a Quartz Crystal Microbalance"Colloids and Surfaces A : Physicochemical and Engineering Aspects. 193.

M.Tanaka、A.Mochizuki、T.Motomura、K.Shimura、M.Onishi、Y.Okahata：“通过石英晶体微天平对聚（2-甲氧基乙基丙烯酸酯）表面上的蛋白质吸附进行原位研究”胶体和表面 A

H. Furusawa, Y. Kitamura, N. Hagiwara, T. Turimoto, and Y. Okahata: "Binding Kinetics of the Toroidal-Shaped PCNA to DNA Strands on a 27 MHz Quartz-Crystal Microbalance"Chem PhysChem.. No.5. 446-448 (2002)

H. Furusawa、Y. Kitamura、N. Hagiwara、T. Turimoto 和 Y. Okahata：“27 MHz 石英晶体微天平上环形 PCNA 与 DNA 链的结合动力学”Chem PhysChem.. No.5。

H.Matsuno, Y.Okahata: "Direct Monitoring of DNA Cleavages catalyzed by an ATP-dependent Deoxyribonuclease on a 27 MHz Quartz-Crystal Microbalance"Chem.Commun. 470-471 (2002)

H.Matsuno、Y.Okahata：“在 27 MHz 石英晶体微天平上直接监测 ATP 依赖性脱氧核糖核酸酶催化的 DNA 裂解”Chem.Commun。

Y. Hoshino, S. Tajima, H. Nakayama, and Y. Okahata: "A RNA-aligned Film prepared from a RNA-Lipid Complex"Macromolecular rapid Commun.. 23. 253-255 (2002)

Y. Hoshino、S. Tajima、H. Nakayama 和 Y. Okahata：“由 RNA-脂质复合物制备的 RNA 排列薄膜”Macromolecular rapid Commun. 23. 253-255 (2002)