Development of 96-type and multi-type quartz-crystal microbalance

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The purpose of this research is to develop a multi-type (96 hole)quartz-crystal microbalance (QCM In this paper, we introduce a 27-MHz quartz-crystal microbalance (QCM) as a new tool to detect DNA-protein/peptide interactions in an aqueous buffer solution. From the time course of the frequency decrease (mass increase), the binding amount (Δm), association constant (Ka), binding and dissociation rate constant (k1 and k1) can be obtained. When dsDNA was immobilized on a QCM, binding kinetics of antibiotics, a Zinc-finger type peptide, a restriction enzyme, and a polymerase to DNA strands could be obtained in aqueous buffer solution. The 27-MHz QCM is highly sensitive and quantitative enough to detect various molecular interactions on DNA double strands.