Studies on the action of apoptosis inducing factor derived from bovine colostrum

牛初乳细胞凋亡诱导因子的作用研究

• 批准号: 08456136
• 负责人: SASAKI Yasuyuki
• 金额: $ 4.29万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08456136/
• 关键词: IGF-I; colostrum; apoptosis; tyrosine kinase; ペプチド

We found a cell-growth inhibiting factor in partially purified fraction from bovine colostrum using an ion-exchange chromatography. The factor induced apoptosis in mouse LM fibroblasts. It is well known that bovine colostrum contains IGF-I,so we measured IGF-I concentrations of the fraction. The fraction contained about 500ng/mg protein of IGF-I.Then, effects of IGF-I on cell proliferation were determined using three cell lines, LM,L6, and Balb/c 3T3 cells. To our surprise, IGF-I induced cell death of LM cells, while it stimulated proliferation of L6 and Balb/c 3T3 cells in low FCS condition. Herbimycin A,a tyrosine kinase inhibitor, inhibited cell death induced by IGF-I in LM cells.These data suggests that IGF-I induces cell death of LM cells and the action involves activation of tyrosine kinase.

用离子交换层析法从牛初乳中部分纯化出一种细胞生长抑制因子。该因子诱导小鼠LM成纤维细胞凋亡。众所周知，牛初乳中含有IGF-I，因此我们测定了其组分的IGF-I浓度。然后，用LM、L 6和Balb/c 3 T3三种细胞系测定IGF-I对细胞增殖的影响。在低FCS条件下，IGF-I可诱导LM细胞死亡，而对L 6和Balb/c 3 T3细胞增殖有促进作用。酪氨酸激酶抑制剂Herbimycin A可抑制IGF-Ⅰ诱导的LM细胞死亡，提示IGF-Ⅰ诱导LM细胞死亡的作用与酪氨酸激酶的激活有关。