Studies on the action of cell growth inhibiting factor partially purified from bovine colostrum.

从牛初乳中部分纯化的细胞生长抑制因子的作用研究。

• 批准号: 06556047
• 负责人: SASAKI Yasuyuki
• 金额: $ 3.71万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06556047/
• 关键词: BOVINE COLOSTRUM; IGF-I; CELL DEATH; LM CELLS; 初乳; 細胞増殖; ペプチド

It has been reported that bovine colostrum contains high levels of growth promoting factors that stimulate DNA synthesis and cell division. Human milk sampled at 1-day postpartum has also been reported to contain the highest level of immunoreactive IGF-I,levels falling rapidly thereafter, by 80% within 48-h after delivery. In this study, we found a cell-growth inhibiting factor in partially purified fraction from bovine colostrum with ion-exchange chromatography. The activity was measured in three cell lines, mouse LM fibroblast, rat L6 myoblast, and Balb/c 3T3 cells. The cell-growth inhibiting activity was observed only in LM cells, whereas the fraction stimulated cell proliferation in L6 and Balb/c 3T3 cells in low FCS condition. IGF-I concentration of the fraction was determined by RIA,and it contained about 50ng/ml of IGF-I.Then, effects of IGF-I on cell growth of these three cell lines were studied. To our surprise, IGF-I inhibited proliferation of LM cells, while it stimulated cell growth of L6 and Balb/c 3T3 cells in low FCS condition. These data suggest that the inhibitory action of the fraction to LM cells is mainly due to IGF-I.

据报道，牛初乳含有高水平的生长促进因子，刺激DNA合成和细胞分裂。据报道，产后1天的人乳中含有最高水平的免疫反应性IGF-I，此后水平迅速下降，在分娩后48小时内下降80%。本研究利用离子交换层析法从牛初乳中分离纯化出一种细胞生长抑制因子。在小鼠LM成纤维细胞、大鼠L 6成肌细胞和Balb/c 3 T3细胞这三种细胞系中测量活性。在低FCS条件下，仅在LM细胞中观察到细胞生长抑制活性，而在L 6和Balb/c 3 T3细胞中观察到细胞增殖刺激活性。用放射免疫分析法测定其IGF-I浓度，其含量约为50 ng/ml。在低FCS条件下，IGF-Ⅰ对LM细胞增殖有抑制作用，而对L 6和Balb/c 3 T3细胞有促进作用。这些数据表明，该组分对LM细胞的抑制作用主要是由于IGF-I。