Pharmacological studies on the NO-dependent and -independent NANC neurotransmitters in the human and cat airway.
对人类和猫气道中 NO 依赖性和非依赖性 NANC 神经递质的药理学研究。
基本信息
- 批准号:08457029
- 负责人:
- 金额:$ 4.93万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Neurally mediated relaxation of airway smooth muscle, in various animal species including man, is largely non-adrenergic, non-cholinergic (NANC). Although the neurotransmitter (s) responsible for the NANC relaxation in the airways have not been conclusively identified, vasoactive intestinal polypeptide (VIP) and nitric oxide (NO) have emerged as strong candidate for this role. The present study were carried out to examine whether and to what extent NO-related compounds and VIP mediate the NANC relaxation in cat and human airway. NANC relaxation evoked by EFS in the cat peripheral airway was biphasic, comprising an initial fast followed by a second slow component and L-NAME or L-NNA selectively abolished the first component without affecting the second one. C-PTIO,a newly synthesized NO scavenger, dose-dependently suppressed the initial component and at a concentration of 10^<-4>M almost halved the amplitude of NANC relaxation. Additional application of L-NAME further reduced the C-PTIO … More resistant NANC relaxation, thereby suggesting that the initial component of the NANC relaxation is due to NO free radial itself and NO-donors. The amplitude of the L-NAME insensitive NANC relaxation showed a fade phenomenon during the first 1 hr of stimulation at an interval of every 20min. Application of VIP (10-28) , a VIP antagonist, enhanced the rate of decrease in the amplitude of L-NAME-insensitive NANC relaxation. Zaprinast, a specific PDE tupe V inhibitor, preferentially enhanced the amplitude of the firstcomponent of the NANC relaxation, and enhanced the accumulation of [cGMP] i evoked by EFS.Rolipram, a specific PDE type IV inhibitor, enhanced both the first ad second components of the NANC relaxation to a similar extent, and enhanced the accumulator of [cAMP] i, thereby indicating that NANC relaxation is associated with concomitant accumulation in both [cAMP] i and [cGMP] i. Taken together, the present study have shown that at least NO free radical itself and NO donors such as nitrosocystine, and VIP are involved as neurotransmitters in the NANC relaxation in the airway. Less
在包括人类在内的各种动物中,神经介导的气道平滑肌松弛主要是非肾上腺素、非胆碱能(NANC)的。尽管引起呼吸道NANC松弛的神经递质(S)尚未确定,但血管活性肠肽(VIP)和一氧化氮(NO)已成为这一作用的有力候选者。本研究旨在探讨一氧化氮相关化合物和血管活性肠肽是否以及在多大程度上介导猫和人呼吸道NANC的松弛。电刺激猫外周气道诱发的NANC松弛是双相的,先快后慢,L或L选择性地取消第一个慢成分而不影响第二个慢成分。新合成的NO清除剂C-PTIO呈剂量依赖性地抑制起始组分,当浓度为10^~(-4)~(-4)时,其幅度几乎减半。L名字的额外应用进一步降低了C-PTIO的…更多的抵抗纳米松弛,从而表明,纳米松弛的初始组成部分是由于没有自由的径向本身和没有施主。在刺激的前1h,每隔20min刺激L名字不敏感的Nanc松弛的幅度出现一次衰减现象。应用血管活性肠肽拮抗剂血管活性肠肽(10-28),可增加L不敏感的NANC松弛幅度的下降速度。特异性PDE TUPE V抑制剂扎普司特优先增强EFS引起的NANC松弛第一组分的幅度,增强EFS引起的[cGMP]i的蓄积。罗利普兰对NANC松弛的第一组分和第二组分均有类似程度的增强作用,并增强[cAMP]i的蓄积量,从而表明NANC松弛与[cAMP]i和[cGMP]i的伴随蓄积有关。较少
项目成果
期刊论文数量(20)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
H.Aizawa: "L-NAME-sensitive and -insensitive nonadrenergic non-cholinergic relaxation of cat airway in vivo and in vitro." Eur.Respir.J.10 :. 314-321 (1997)
H.Aizawa:“体内和体外对猫气道的 L-NAME 敏感和不敏感的非肾上腺素能非胆碱能松弛作用。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Y.Waniishi: "Preferential potentiation by hypotonic cell swelling of muscarinic cation current guinea pig ileum." Am.J.Physiol.272. C240-C253 (1997)
Y.Waniishi:“毒蕈碱阳离子电流豚鼠回肠的低渗细胞肿胀优先增强。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
V.Bauer: "Hydrogen peroxide induced responses of cat tracheal smooth muscle cells." Br.J.Pharmacol.121. 867-874 (1997)
V.Bauer:“过氧化氢诱导猫气管平滑肌细胞的反应。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
H.Tanaka: "The possible role of nitric oxide in relaxations and excitatory neuroeffector transmission in the cat airway" J.Physiol.493・3. 785-791 (1996)
H.Tanaka:“一氧化氮在猫气道的放松和兴奋性神经效应器传递中的可能作用”J.Physiol.493·3(1996)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
K.Yamada: "Electrophysiological characterization of a motilin agonist,GM611,on rabbit duodenal smooth muscle." Am.J.Physiol.271. G1003-G1016 (1996)
K.Yamada:“胃动素激动剂 GM611 对兔十二指肠平滑肌的电生理学特征。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
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{{ truncateString('ITO Yushi', 18)}}的其他基金
Pharmacological study on the mechano-sensitive molecules involved in vascular smooth muscle and endothelial cells.
参与血管平滑肌和内皮细胞的力敏感分子的药理学研究。
- 批准号:
17390067 - 财政年份:2005
- 资助金额:
$ 4.93万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
cDNA cloning of the new nigedipine-insenstive voltage-dependent Ca^2+ channels in the peripheral resistant artery..
外周阻力动脉中新型尼格地平不敏感电压依赖性 Ca^2 通道的 cDNA 克隆。
- 批准号:
14370033 - 财政年份:2002
- 资助金额:
$ 4.93万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Elucidation of roles and development of selective blockers for novel voltage-gated Ca2+ channels in peripheral resistant arterioles
外周抵抗小动脉中新型电压门控 Ca2 通道选择性阻断剂的作用和开发的阐明
- 批准号:
12470020 - 财政年份:2000
- 资助金额:
$ 4.93万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Specific interaction of FK506 binding protein (FKBP) isoforms with the ryanodine/CaィイD12+ィエD1 release channel subtypes
FK506 结合蛋白 (FKBP) 亚型与兰尼定/CaD12+D1 释放通道亚型的特异性相互作用
- 批准号:
10470024 - 财政年份:1998
- 资助金额:
$ 4.93万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Pharmacological studies on the active factors derived from airway epithelial cells.
气道上皮细胞活性因子的药理学研究。
- 批准号:
06454162 - 财政年份:1994
- 资助金额:
$ 4.93万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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- 批准号:
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