Cellular receptor for measles virtus

麻疹病毒的细胞受体

• 批准号: 08457097
• 负责人: YANAGI Yusuke
• 金额: $ 4.22万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457097/
• 关键词: measles virus; viral receptor; CD46; cell fusion; syncytium

Measles virus (MV) is efficiently isolated from patients with measles by using B95a cells, a marmoset B cell line. Recent wildtype MV strains isoleted using B95a cells did not produce cytopathic efffects in any of CD46^+ primate cell lines examined (except B95a cells), nor did they induce downregulation of CD46. Transfection of the hemagglutinin (H) and fusion (F) genes of the Edmonston strain of MV produced syncytia in HeLa, Cos and B95a cells. By contrast, the expression of the H gene from the two wildtype strains, together with the F gene of the Edmonston strain, resulted in syncytium production in B95a cells, but not in HeLa and Cos cells. Cocultivation of Cos cells expressing the wild-type H protein and the Edmonston strain F protein with B95a cells, but not with Hela, results suggest that these recent MV isolates may use a molecule Jurkat or BJAB cells, generated large syncytia. The results suggest that these recent MV isolates may use a molecule other than CD46 as the vellular receptor or require another coreceptor to infect cells. We are currently in a process of identifying the molecule present in B95a cells that enables these recent wild-type MV strains to infect cells.

用绒猴B细胞系B95 a细胞从麻疹患者中有效分离麻疹病毒（MV）。最近用B95a细胞分离的野生型MV株在任何检测的CD 46 ^+灵长类细胞系中均未产生致细胞病变效应（B95a细胞除外），也未诱导CD 46下调。MV Edmonston株的血凝素（H）和融合（F）基因的转染在HeLa、Cos和B95a细胞中产生合胞体。相比之下，来自两种野生型菌株的H基因的表达，以及Edmonston菌株的F基因，导致B95a细胞中合胞体的产生，但在HeLa和Cos细胞中没有。将表达野生型H蛋白和Edmonston株F蛋白的Cos细胞与B95 a细胞共培养，但不与Hela共培养，结果表明这些最近的MV分离物可能使用分子Jurkat或BJAB细胞，产生大的合胞体。结果表明，这些最近的MV分离株可能使用的分子以外的CD46作为vellular受体或需要另一个辅助受体感染细胞。我们目前正在鉴定B95a细胞中存在的分子，该分子使这些最近的野生型MV菌株能够感染细胞。

项目成果

柳 雄介: "Key Word 1997-'98感染症" 先端医学社,東京, 205 (1996)

Yusuke Yanagi：“关键字 1997-98 传染病”Senshin Igakusha，东京，205 (1996)

Yanagi, Y.: Viral receptors (in Japanese). Key Words 1997-'98 Infections Diseases, K.Shimizu et al. (Ed.), Sentan igaku sha Tokyo, Japan, 28-29 (1996)

Yanagi, Y.：病毒受体（日语）。

Miyagawa,S.: "Difference of expression levels between gene technological product ΔCYT-MCP(CD46)and intact MCP(CD46)in transgenic mice" Transplant.Proc.28. 585-586

Miyakawa, S.：“转基因小鼠中基因技术产品 ΔCYT-MCP(CD46) 和完整 MCP(CD46) 之间的表达水平差异”Transplant.Proc.28。

Tanaka, K.: "The hemagglutinin of recent measles virus isolates induces cell fusion in a marmoset cell line,but not in other CD46-positive human and monkey cell lines, when expressed together with the F protein." Arch.Virol.(1998)

Tanaka, K.：“最近分离的麻疹病毒的血凝素与 F 蛋白一起表达时，会在狨猴细胞系中诱导细胞融合，但不会在其他 CD46 阳性人和猴细胞系中诱导细胞融合。”

Seya, T.et al.: "The CD46 taransmembrane domain is required for efficient formation of measles-virus mediated syncytium" Biochem.J.322. 135-144 (1997)

Seya, T.等人：“CD46 taransmembrane 结构域是麻疹病毒介导的合胞体有效形成所必需的”Biochem.J.322。