Cellular receptor for measles virtus

利用狨猴B细胞系B95a细胞，从麻疹患者体内有效分离出麻疹病毒。最近用B95a细胞分离的野生型MV株在检测的任何CD46 +灵长类细胞系（B95a细胞除外）中都没有产生细胞病变作用，也没有诱导CD46的下调。转染MV Edmonston菌株的血凝素(H)和融合(F)基因在HeLa、Cos和B95a细胞中产生合胞体。相比之下，两个野生型菌株的H基因和Edmonston菌株的F基因的表达在B95a细胞中产生合胞体，而在HeLa和Cos细胞中没有产生合胞体。将表达野生型H蛋白和Edmonston菌株F蛋白的Cos细胞与B95a细胞共培养，而不是与Hela细胞共培养，结果表明这些新近分离的MV可能使用Jurkat或BJAB细胞分子，产生了较大的合胞体。结果表明，这些最近的MV分离株可能使用CD46以外的分子作为细胞受体或需要另一种辅助受体来感染细胞。我们目前正在鉴定B95a细胞中存在的分子，使这些最近的野生型MV菌株能够感染细胞。

Measles virus (MV) is efficiently isolated from patients with measles by using B95a cells, a marmoset B cell line. Recent wildtype MV strains isoleted using B95a cells did not produce cytopathic efffects in any of CD46^+ primate cell lines examined (except B95a cells), nor did they induce downregulation of CD46. Transfection of the hemagglutinin (H) and fusion (F) genes of the Edmonston strain of MV produced syncytia in HeLa, Cos and B95a cells. By contrast, the expression of the H gene from the two wildtype strains, together with the F gene of the Edmonston strain, resulted in syncytium production in B95a cells, but not in HeLa and Cos cells. Cocultivation of Cos cells expressing the wild-type H protein and the Edmonston strain F protein with B95a cells, but not with Hela, results suggest that these recent MV isolates may use a molecule Jurkat or BJAB cells, generated large syncytia. The results suggest that these recent MV isolates may use a molecule other than CD46 as the vellular receptor or require another coreceptor to infect cells. We are currently in a process of identifying the molecule present in B95a cells that enables these recent wild-type MV strains to infect cells.