Tropism and pathogenesis of measles virus

麻疹病毒的趋向性和发病机制

• 批准号: 13470064
• 负责人: YANAGI Yusuke
• 金额: $ 10.5万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13470064/
• 关键词: measles virus; receptor; SLAM; CD150; canine distemper virus; rinderpest virus

1. By titrating viruses on Vero and Vero/SLAM cells, we show that the great majority of measles viruses in measles patients use SLAM but not CD46 as a cellular receptor, thereby proving that SLAM is the principal receptor for measles virus.2.We show that although monocytes freshly isolated from peripheral blood mononuclear cells do not express SLAM, its expression is readily induced after stimulation with mitogens or even with measles virus particles. Furthermore, mature dendritic cells, but not immature dendritic cells, also express SLAM. These results indicate that MV infection of monocytes/macrophages and dendritic cells, like that of lymphocytes, is mediated by SLAM.3. We show that canine distemper virus and rinderpest virus respectively use canine and bovine SLAMs as cellular receptors.4. By using the recombinant measles virus expressing the green fluorescent protein, we show that wild-type measles virus strains can infect SLAM-negative cells with 2 to 3 logs lower efficiency than it does SLAM-positive cells, indicating the presence of SLAM- and CD46-independent measles virus infection.5. We show that the interaction of the measles virus H protein with SLAM results in its downregulation from the cell surface, suggesting its involvement in measles virus-induced immunosuppression.

1.通过在Vero和Vero/SLAM细胞上滴定病毒，我们发现麻疹患者中的绝大多数麻疹病毒使用SLAM而不是CD 46作为细胞受体，从而证明SLAM是麻疹病毒的主要受体。2.我们发现，尽管从外周血单核细胞中新鲜分离的单核细胞不表达SLAM，它的表达在用有丝分裂原或甚至用麻疹病毒颗粒刺激后容易被诱导。此外，成熟的树突状细胞，而不是未成熟的树突状细胞，也表达SLAM。这些结果表明，MV感染单核细胞/巨噬细胞和树突状细胞，像淋巴细胞一样，是由SLAM介导的。我们发现犬瘟热病毒和牛瘟病毒分别利用犬和牛的SLAMs作为细胞受体.通过使用表达绿色荧光蛋白的重组麻疹病毒，我们表明野生型麻疹病毒株可以感染SLAM阴性细胞，效率比SLAM阳性细胞低2至3个对数，表明存在SLAM和CD 46非依赖性麻疹病毒感染.我们发现，麻疹病毒H蛋白与SLAM的相互作用导致其从细胞表面下调，这表明其参与麻疹病毒诱导的免疫抑制。

项目成果

Tatsuo H, Yanagi Y: "The morbillivirus receptor SLAM (CD150)"Microbiology and Immunology. 46. 135-142 (2002)

Tatsuo H、Yanagi Y：“麻疹病毒受体 SLAM (CD150)”微生物学和免疫学。

Tatsuo H, Ono N, Yanagi Y: "Morbilliviruses Use Signaling Lymphocyte Activation Molecules (CD150) as Cellular Receptors"Journal of Virology. 75. 5842-5850 (2001)

Tatsuo H、Ono N、Yanagi Y：“麻疹病毒使用信号淋巴细胞激活分子 (CD150) 作为细胞受体”病毒学杂志。

Minagawa H, Tanaka K, Ono N, Tatsuo H, Yanagi Y: "Induction of the measles virus receptor SLAM (CD150) on monocytes"Journal of General Virology. 82. 2913-2917 (2001)

Minakawa H、Tanaka K、Ono N、Tatsuo H、Yanagi Y：“单核细胞上麻疹病毒受体 SLAM (CD150) 的诱导”普通病毒学杂志。

Tatsuo H., Yanagi Y.: "The morbillivirus receptor SLAM (CD150)"Microbiol. Immunol.. 46. 135-142 (2002)

Tatsuo H.，Yanagi Y.：“麻疹病毒受体 SLAM (CD150)”微生物学。

Vincent S, Tigaud I, Schneider H, Buccholz C, Yanagi Y, Gerlier D.: "Restriction of measles virus RNA synthesis by a mouse host cell line : trans-complementation by polymerase components or a human cellular factor(s)"Journal of Virology. 76(12). 6121-6130

Vincent S、Tigaud I、Schneider H、Buccholz C、Yanagi Y、Gerlier D.：“小鼠宿主细胞系对麻疹病毒 RNA 合成的限制：聚合酶成分或人类细胞因子的反式互补”杂志